##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549804_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744380
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36724388081356	33.0	31.0	34.0	30.0	34.0
2	32.478900561541145	34.0	31.0	34.0	31.0	34.0
3	32.48676885461727	34.0	31.0	34.0	30.0	34.0
4	35.980589215185795	37.0	35.0	37.0	35.0	37.0
5	36.01716999382036	37.0	35.0	37.0	35.0	37.0
6	36.03628120046213	37.0	35.0	37.0	35.0	37.0
7	36.17824901260109	37.0	36.0	37.0	35.0	37.0
8	35.85378167065208	37.0	36.0	37.0	35.0	37.0
9	37.728336333593056	39.0	38.0	39.0	35.0	39.0
10	37.59790563959268	39.0	37.0	39.0	35.0	39.0
11	37.50772051908971	39.0	37.0	39.0	35.0	39.0
12	37.35465353717188	39.0	37.0	39.0	35.0	39.0
13	37.29730782664768	39.0	37.0	39.0	34.0	39.0
14	38.571983395577526	40.0	38.0	41.0	35.0	41.0
15	38.63509094817163	40.0	38.0	41.0	35.0	41.0
16	38.71727209221097	40.0	38.0	41.0	35.0	41.0
17	38.70588812165829	40.0	38.0	41.0	35.0	41.0
18	38.69768129181332	40.0	38.0	41.0	35.0	41.0
19	38.67801391762272	40.0	38.0	41.0	35.0	41.0
20	38.58792820871061	40.0	38.0	41.0	35.0	41.0
21	38.540756065450445	40.0	38.0	41.0	35.0	41.0
22	38.522493887530565	40.0	37.0	41.0	35.0	41.0
23	38.4733227652543	40.0	37.0	41.0	35.0	41.0
24	38.413620731346896	40.0	37.0	41.0	34.0	41.0
25	38.36011042746984	40.0	37.0	41.0	34.0	41.0
26	38.31653187887907	40.0	37.0	41.0	34.0	41.0
27	38.252451704774444	40.0	37.0	41.0	34.0	41.0
28	38.195969800370776	40.0	37.0	41.0	34.0	41.0
29	38.158111448453745	40.0	37.0	41.0	34.0	41.0
30	38.074641983932935	40.0	36.0	41.0	34.0	41.0
31	38.045424380020954	40.0	36.0	41.0	34.0	41.0
32	37.947431419436306	40.0	36.0	41.0	34.0	41.0
33	37.89299282624466	40.0	36.0	41.0	34.0	41.0
34	37.81784303715844	40.0	36.0	41.0	34.0	41.0
35	37.72237969854107	40.0	36.0	41.0	33.0	41.0
36	37.65944007093152	40.0	36.0	41.0	33.0	41.0
37	37.57068298449717	40.0	36.0	41.0	33.0	41.0
38	37.48510706897015	40.0	35.0	41.0	33.0	41.0
39	37.4323598162229	40.0	35.0	41.0	33.0	41.0
40	37.30944947473065	40.0	35.0	41.0	33.0	41.0
41	37.228199306805664	40.0	35.0	41.0	33.0	41.0
42	37.190711733254524	40.0	35.0	41.0	33.0	41.0
43	37.11287917461512	39.0	35.0	41.0	33.0	41.0
44	37.07107794406083	39.0	35.0	41.0	32.0	41.0
45	37.00962546011446	39.0	35.0	41.0	32.0	41.0
46	36.877929283430504	39.0	35.0	41.0	32.0	41.0
47	36.79295252424837	39.0	35.0	41.0	32.0	41.0
48	36.72283376769929	39.0	35.0	41.0	32.0	41.0
49	36.606202477229374	39.0	35.0	41.0	31.0	41.0
50	36.53693006260244	39.0	35.0	41.0	31.0	41.0
51	35.72674440473951	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	13.0
10	7.0
11	14.0
12	14.0
13	27.0
14	26.0
15	36.0
16	50.0
17	89.0
18	145.0
19	310.0
20	504.0
21	862.0
22	1368.0
23	1839.0
24	2494.0
25	3463.0
26	4423.0
27	5323.0
28	5888.0
29	6850.0
30	8274.0
31	10694.0
32	13997.0
33	19825.0
34	39466.0
35	61553.0
36	48182.0
37	72599.0
38	130204.0
39	305138.0
40	696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.556758644778206	21.644590128697708	23.91036836024611	11.888282866277976
2	33.735592036325535	25.162820065020554	23.18412638706037	17.91746151159354
3	31.461350385555765	25.782664767994838	25.038286896477608	17.71769794997179
4	29.69961578763535	25.10962142991483	26.513474300760365	18.677288481689462
5	27.439614175555494	29.339987640721137	23.188559606652518	20.03183857707085
6	26.74870361911927	33.77266987291437	23.48182379967221	15.99680270829415
7	79.21840995190628	6.360461054837582	9.319567962599749	5.101561030656385
8	79.95862328380666	6.5977054730110964	8.51702087643408	4.9266503667481665
9	73.47282302050027	8.210994384588517	10.613262043579892	7.702920551331309
10	43.668153362529885	23.76474381364357	17.59840404094683	14.968698782879711
11	32.36787662215535	26.09352749939547	22.95265858835541	18.585937290093767
12	30.577661946855102	22.240387973884307	26.834412531234047	20.347537548026544
13	25.634219081651842	24.365512238372876	28.36078347080792	21.63948520916736
14	20.462532577447003	28.229667642870577	28.432118004245144	22.875681775437275
15	19.680673849378007	26.185684730917004	33.1772750476907	20.956366372014294
16	22.226953975120235	25.058437894623715	30.80308444611623	21.911523684139823
17	22.23138719471238	25.378301405196268	28.201993605416586	24.188317794674763
18	22.582551922405223	26.160966173191113	28.31040597544265	22.946075928961015
19	24.55667804078562	26.752062118810283	26.380746392971332	22.31051344743276
20	27.404148418818348	26.009430667132378	27.141245063005453	19.44517585104382
21	25.26223165587469	26.8481152099734	26.621752330798788	21.267900803353125
22	23.21730836400763	25.75002015099815	26.52207205996937	24.510599425024854
23	24.11349042155888	26.32123377844649	27.297885488594538	22.267390311400092
24	23.55235229318359	25.17128348424192	28.174185227974956	23.10217899459953
25	22.96931674682286	27.708428490824577	26.137591015341627	23.184663747010937
26	22.220102635750557	28.430237244418173	26.91971842338591	22.42994169644536
27	23.66761600257933	27.92283511109917	27.227760015046076	21.181788871275423
28	21.603616432467287	28.013514602756658	28.608640748005048	21.774228216771004
29	23.42432628496198	25.74343749160375	28.466106021118247	22.366130202316022
30	23.14261533087939	25.92130363524007	28.359843090894437	22.57623794298611
31	23.743518095596336	27.34638222413284	25.994653268491902	22.91544641177893
32	24.884064590666057	27.485827131303903	25.837072462989337	21.793035815040703
33	24.18603401488487	27.233267954539347	26.50380182165023	22.076896208925547
34	24.011526370939574	25.93648405384347	27.919879631371074	22.132109943845883
35	23.832988527365057	25.202987721325133	26.585077514172866	24.378946237136947
36	24.858136973051398	27.145275262634676	26.83172573148123	21.164862032832694
37	24.289072785405306	27.600016120798514	28.01190252290497	20.09900857089121
38	22.93935892957898	26.267497783390205	27.41570165775545	23.37744162927537
39	25.12251806872834	25.16752196458798	27.641795856954783	22.0681641097289
40	24.86418227249523	24.361885058706577	29.05088798731831	21.723044681479887
41	22.385072140573364	25.9359466938929	28.072892877293853	23.606088288239878
42	23.90848760041914	24.83234369542438	27.69741261183804	23.56175609231844
43	23.94355033719337	26.372282973749968	27.52438270775679	22.159783981299874
44	23.37421746957199	26.019774846180717	27.374459281549747	23.23154840269755
45	23.026142561594884	25.18068728337677	26.752330798785568	25.040839356242778
46	23.458986001773287	27.013622074746767	26.736881700206883	22.79051022327306
47	23.30503237593702	25.84728230205003	28.57371235121846	22.273972970794485
48	23.148123270372658	24.026572449555335	28.719471237808648	24.10583304226336
49	22.356054703242968	25.258470136220744	29.067814825761033	23.31766033477525
50	21.590048093715573	24.76705446143099	29.043230608022785	24.59966683683065
51	21.14900991429109	25.207958300867837	26.86638544829254	26.776646336548538
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	819.5
2	1374.0
3	2619.5
4	3865.0
5	2666.0
6	1467.0
7	1443.5
8	1420.0
9	1385.5
10	1351.0
11	1382.0
12	1413.0
13	1366.5
14	1320.0
15	1348.5
16	1377.0
17	1547.5
18	1718.0
19	1709.0
20	1700.0
21	2470.0
22	3240.0
23	3450.0
24	3660.0
25	4233.5
26	5495.0
27	6183.0
28	7877.0
29	9571.0
30	10935.0
31	12299.0
32	14257.5
33	16216.0
34	17501.0
35	18786.0
36	20429.0
37	22072.0
38	23918.0
39	25764.0
40	29093.5
41	32423.0
42	36130.0
43	39837.0
44	42852.5
45	45868.0
46	54402.5
47	62937.0
48	63123.0
49	63309.0
50	63322.5
51	63336.0
52	55612.0
53	47888.0
54	43734.0
55	39580.0
56	39306.0
57	39032.0
58	38158.0
59	37284.0
60	36618.5
61	35953.0
62	33057.0
63	30161.0
64	26468.0
65	22775.0
66	18539.5
67	14304.0
68	12161.0
69	10018.0
70	8431.0
71	6844.0
72	5959.5
73	5075.0
74	4175.0
75	2801.0
76	2327.0
77	1895.5
78	1464.0
79	1019.5
80	575.0
81	388.0
82	201.0
83	118.5
84	36.0
85	21.5
86	7.0
87	4.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	744380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.57856520245602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24483932187937	17.756744938137707
2	10.468842226922575	5.146182425372828
3	3.698974634765024	2.7274646772840927
4	1.8355855902618827	1.8046424045976162
5	1.0372527130407174	1.2747091719447838
6	0.7401847190036418	1.091560702673556
7	0.5746071375409934	0.9886113297093544
8	0.4942338895253498	0.9718047903169805
9	0.4114829594440972	0.9102294673556678
>10	7.424986739183781	47.53174415972047
>50	0.9954568666211318	14.771891782122276
>100	0.06636379110807365	3.0279149881623937
>500	0.003871221147970963	0.5774188754585136
>1k	0.0033181895554036827	1.4190802871438046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	2245	0.3015932722534189	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2170	0.29151777318036487	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	1890	0.25390257664096294	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	1849	0.2483946371476934	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1269	0.17047744431607512	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1017	0.13662376743061339	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09524705123727129	0.0
2	0.0	0.0	0.0	0.5334640909213036	0.0
3	0.0	0.0	0.0	0.8042935060049975	0.0
4	0.0	0.0	0.0	1.2003277895698434	0.0
5	0.0	0.0	0.0	2.4498240146161905	0.0
6	0.0	0.0	0.0	3.074504957145544	0.0
7	0.0	0.0	0.0	3.7178591579569575	0.0
8	0.0	0.0	0.0	4.572933179290147	0.0
9	0.0	0.0	0.0	4.945860984980789	0.0
10	1.3433998764072113E-4	0.0	0.0	5.9496493726322575	0.0
11	1.3433998764072113E-4	0.0	0.0	7.1433945028077055	0.0
12	1.3433998764072113E-4	0.0	0.0	8.245922781375103	0.0
13	1.3433998764072113E-4	0.0	0.0	8.642628764878154	0.0
14	1.3433998764072113E-4	0.0	0.0	8.819285848625702	0.0
15	1.3433998764072113E-4	0.0	0.0	9.14855315833311	0.0
16	1.3433998764072113E-4	0.0	0.0	9.75765066229614	0.0
17	1.3433998764072113E-4	0.0	0.0	10.368360246110857	0.0
18	1.3433998764072113E-4	0.0	0.0	11.029178645315564	0.0
19	1.3433998764072113E-4	0.0	0.0	11.511190520970471	0.0
20	1.3433998764072113E-4	0.0	0.0	11.921061823262312	0.0
21	1.3433998764072113E-4	0.0	0.0	12.416642037668932	0.0
22	1.3433998764072113E-4	0.0	0.0	12.954808028157661	0.0
23	1.3433998764072113E-4	0.0	0.0	13.46691206104409	0.0
24	1.3433998764072113E-4	0.0	0.0	13.87839544318762	0.0
25	1.3433998764072113E-4	0.0	0.0	14.250785888927698	0.0
26	1.3433998764072113E-4	0.0	0.0	14.59227813751041	0.0
27	1.3433998764072113E-4	0.0	0.0	14.943039845240333	0.0
28	2.6867997528144227E-4	0.0	0.0	15.268679975281442	0.0
29	2.6867997528144227E-4	0.0	0.0	15.623606222628228	0.0
30	2.6867997528144227E-4	0.0	0.0	16.01292350681104	0.0
31	2.6867997528144227E-4	0.0	0.0	16.389478492167978	0.0
32	2.6867997528144227E-4	0.0	0.0	16.756360998414788	0.0
33	2.6867997528144227E-4	0.0	0.0	17.136140143475107	0.0
34	4.030199629221634E-4	0.0	0.0	17.49617131035224	0.0
35	4.030199629221634E-4	0.0	0.0	17.884010854671	0.0
36	4.030199629221634E-4	0.0	0.0	18.258685080200973	0.0
37	4.030199629221634E-4	0.0	0.0	18.634031005669147	0.0
38	4.030199629221634E-4	0.0	0.0	19.004675031569896	0.0
39	4.030199629221634E-4	0.0	0.0	19.377468497272897	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAA	30	2.1644664E-6	45.000004	27
TATACGG	30	2.1644664E-6	45.000004	2
CACCCGT	60	0.0	45.000004	38
CTTAACG	20	7.0316833E-4	45.0	1
ACGCCTA	20	7.0316833E-4	45.0	43
CCGTCGA	25	3.889477E-5	45.0	41
TCTACGA	20	7.0316833E-4	45.0	36
CTACGAT	20	7.0316833E-4	45.0	37
TTGCGTC	20	7.0316833E-4	45.0	16
TCGGTAC	25	3.889477E-5	45.0	36
ACGACGT	20	7.0316833E-4	45.0	32
ATAACGG	65	0.0	41.538464	2
TTAACGG	50	1.0804797E-9	40.5	2
AATGCGG	180	0.0	40.0	2
ATACGAG	45	1.9266736E-8	40.0	1
TAACGGG	175	0.0	39.857143	3
CGTAAGG	80	0.0	39.375	2
TCGTCGG	35	6.245322E-6	38.57143	1
CATCGGT	35	6.245322E-6	38.57143	24
TACTACG	35	6.245322E-6	38.57143	1
>>END_MODULE