FastQCFastQC Report
Sat 18 Jun 2016
SRR3549802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797247
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC26830.33653309451148766TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC25910.3249933834809037TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGCT22550.2828483518909447TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13900.1743499818751278No Hit
TCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC11780.14775847384812987TruSeq Adapter, Index 13 (95% over 21bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC9700.1216686923876791No Hit
ACTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCCGTCTTCTGC8390.10523714733326059TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCTTCCCTCGTATGCC8290.10398283091689277No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG453.8380676E-1045.01
CTTCGAC207.031985E-445.021
TCGTTGA253.8897262E-545.024
TCGTTAC253.8897262E-545.037
CGAAATC207.031985E-445.015
ATAATCG253.8897262E-545.024
CGTTGAT253.8897262E-545.025
ATTCGCG207.031985E-445.01
GCGCATA207.031985E-445.025
ATCGTTA253.8897262E-545.036
GTTAACG207.031985E-445.01
TGTTCGA207.031985E-445.036
ACGTGCG253.8897262E-545.01
CCGACAC207.031985E-445.024
CATCGTA253.8897262E-545.032
CGTAATC207.031985E-445.030
CGTAAGA207.031985E-445.045
TATAGCG253.8897262E-545.01
ATGCGAT253.8897262E-545.011
TACTACG207.031985E-445.030