FastQCFastQC Report
Sat 18 Jun 2016
SRR3549797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691608
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16190.2340921446831153No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC13320.1925946489919145No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC8920.12897479497056136No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC8700.1257938022694937No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG8390.12131149437253474No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7820.11306983146522308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG253.8891885E-545.01
TAGCGAT207.031336E-445.040
ATCCGGC253.8891885E-545.035
TACGCTA253.8891885E-545.039
CATACGT207.031336E-445.034
ACGTCCA253.8891885E-545.015
TACTACG253.8891885E-545.036
TCGACAA502.1827873E-1145.019
TAAGACG253.8891885E-545.016
AATCCGG302.1642427E-644.9999962
TACGTAG302.1642427E-644.9999961
CTTACGT302.1642427E-644.99999622
CTACGTA900.042.521
TACGTAT900.042.522
TCGATGG650.041.538462
TTAATCG1000.040.520
CCCGGTT501.0804797E-940.519
CCTACGT950.040.26315320
TACGAAT950.040.26315312
CGAATAT950.040.26315314