##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	691608
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3272952308244	33.0	31.0	34.0	30.0	34.0
2	32.44830742270188	34.0	31.0	34.0	31.0	34.0
3	32.46388705740824	34.0	31.0	34.0	30.0	34.0
4	35.94855178077755	37.0	35.0	37.0	35.0	37.0
5	35.99650524574614	37.0	35.0	37.0	35.0	37.0
6	36.016483325814626	37.0	35.0	37.0	35.0	37.0
7	36.17412609455067	37.0	36.0	37.0	35.0	37.0
8	35.87678280181837	37.0	36.0	37.0	35.0	37.0
9	37.73280095082764	39.0	38.0	39.0	35.0	39.0
10	37.571288938242475	39.0	37.0	39.0	35.0	39.0
11	37.48510138691282	39.0	37.0	39.0	35.0	39.0
12	37.30211188997235	39.0	37.0	39.0	35.0	39.0
13	37.213623902557515	39.0	37.0	39.0	34.0	39.0
14	38.43722744676175	40.0	38.0	41.0	34.0	41.0
15	38.547062787012294	40.0	38.0	41.0	35.0	41.0
16	38.60351384021006	40.0	38.0	41.0	35.0	41.0
17	38.62222530682121	40.0	38.0	41.0	35.0	41.0
18	38.622351100623476	40.0	38.0	41.0	35.0	41.0
19	38.605786804085554	40.0	38.0	41.0	35.0	41.0
20	38.53845530994436	40.0	37.0	41.0	35.0	41.0
21	38.485436837052205	40.0	37.0	41.0	35.0	41.0
22	38.46715625036148	40.0	37.0	41.0	35.0	41.0
23	38.436932481984016	40.0	37.0	41.0	34.0	41.0
24	38.37088495216944	40.0	37.0	41.0	34.0	41.0
25	38.287525592532184	40.0	37.0	41.0	34.0	41.0
26	38.261707499045706	40.0	37.0	41.0	34.0	41.0
27	38.2078547385224	40.0	37.0	41.0	34.0	41.0
28	38.15821968513956	40.0	36.0	41.0	34.0	41.0
29	38.14612179153509	40.0	36.0	41.0	34.0	41.0
30	38.059720246151	40.0	36.0	41.0	34.0	41.0
31	37.99845866444576	40.0	36.0	41.0	34.0	41.0
32	37.937408184983404	40.0	36.0	41.0	34.0	41.0
33	37.89602202403674	40.0	36.0	41.0	34.0	41.0
34	37.81676325317232	40.0	36.0	41.0	34.0	41.0
35	37.736366554464375	40.0	36.0	41.0	33.0	41.0
36	37.68991972331147	40.0	36.0	41.0	33.0	41.0
37	37.64425078946455	40.0	35.0	41.0	33.0	41.0
38	37.552292628194	40.0	35.0	41.0	33.0	41.0
39	37.509676001434336	40.0	35.0	41.0	33.0	41.0
40	37.404510936831265	40.0	35.0	41.0	33.0	41.0
41	37.314425223537036	40.0	35.0	41.0	33.0	41.0
42	37.26779331644516	40.0	35.0	41.0	33.0	41.0
43	37.206605475934346	39.0	35.0	41.0	33.0	41.0
44	37.14989271379163	39.0	35.0	41.0	33.0	41.0
45	37.07902453412916	39.0	35.0	41.0	33.0	41.0
46	36.94769725046558	39.0	35.0	41.0	32.0	41.0
47	36.8724740026142	39.0	35.0	41.0	32.0	41.0
48	36.830169691501546	39.0	35.0	41.0	32.0	41.0
49	36.73086054527999	39.0	35.0	41.0	32.0	41.0
50	36.65717284935975	39.0	35.0	41.0	32.0	41.0
51	35.88900938103666	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	12.0
11	15.0
12	21.0
13	20.0
14	27.0
15	28.0
16	57.0
17	76.0
18	142.0
19	243.0
20	353.0
21	599.0
22	995.0
23	1382.0
24	2032.0
25	2933.0
26	3925.0
27	4873.0
28	5828.0
29	6489.0
30	7784.0
31	9818.0
32	13134.0
33	18791.0
34	38955.0
35	60691.0
36	43895.0
37	65250.0
38	121310.0
39	281394.0
40	521.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.343529860846026	22.67787532822061	25.161941446599805	12.816653364333552
2	33.261327225827344	24.777619692079906	23.653572543984453	18.307480538108294
3	32.30167956414616	23.60484551942719	25.71659090120415	18.376884015222497
4	29.97434963158321	25.138662363651086	24.728892667522643	20.15809533724306
5	25.634463453285676	27.93489953846688	24.709806711316237	21.720830296931208
6	26.29177221778811	34.23355426773548	23.883760743079897	15.590912771396514
7	79.85433945240656	6.2263594406079745	8.818723901400794	5.100577205584667
8	79.95193809209842	5.4639333263929855	8.929191102474233	5.654937479034366
9	73.61915998658199	8.19380342621832	11.287608009161259	6.899428578038426
10	42.54129506888295	24.347028952817205	18.44860094157384	14.663075036726006
11	32.293582491816174	24.74089368544031	23.890556500213993	19.074967322529528
12	30.713352072272155	21.51753594521752	27.29436328093371	20.474748701576615
13	25.54091334975882	25.04106372395924	28.709760442331493	20.708262483950445
14	19.855033487177707	28.07399567384993	29.339307815988246	22.73166302298412
15	19.597517668968546	25.269516836126822	34.22198702154978	20.91097847335485
16	21.208545881481996	24.547865264716428	32.89218748192618	21.351401371875397
17	21.785751466148454	24.4803414651074	28.09438294525222	25.63952412349192
18	22.91355796925426	25.842529294050966	29.19486182924431	22.049050907450464
19	24.438121016529596	27.3759123665429	26.956165920579288	21.22980069634822
20	26.65657424436964	25.30147135371482	27.917404078611007	20.12455032330453
21	24.859168777689096	25.76922187134909	28.03871557298354	21.332893777978278
22	23.15314455587558	24.878399324472824	27.846988467455553	24.121467652196042
23	22.782992677933166	26.546829996182808	28.063295971128156	22.606881354755874
24	23.110056563833847	24.624642861274015	29.08367167528427	23.18162889960787
25	22.904014991151055	26.34324646331448	26.84613249123781	23.906606054296653
26	22.159807289678547	27.938514302899907	26.696047471978346	23.2056309354432
27	22.994673283131483	26.92536812760986	27.830794322795573	22.249164266463083
28	20.902447629292894	27.149483522457807	28.38848017952366	23.559588668725638
29	22.28111878405108	25.062318538825462	29.233322922811766	23.42323975431169
30	23.256093046928317	24.845143491688933	27.28062717608819	24.618136285294558
31	23.533273183653165	26.584278955709014	25.222235717342773	24.660212143295045
32	23.921643474338065	26.56259036911083	26.074886351806224	23.440879804744885
33	22.992359833894344	26.318232293437898	26.249840950364945	24.43956692230281
34	21.400417577587305	25.36913974390117	28.953395565117813	24.277047113393717
35	21.86831268579889	24.001023701287437	28.566037408474166	25.564626204439513
36	22.042544331471007	26.446194954367215	26.70819308047333	24.803067633688446
37	21.108055430243724	26.62302923043111	28.235069576985804	24.03384576233936
38	21.478930261072744	25.669888144729384	27.565904385143032	25.28527720905484
39	22.10283860221397	24.669610530820925	29.818914760962855	23.408636106002245
40	23.168326566494315	23.13102219754543	31.2408184983401	22.459832737620154
41	20.215931568171566	24.624353680119373	29.107529120542274	26.052185631166786
42	22.21533007136991	25.91901770945391	27.144567442828887	24.721084776347297
43	23.000456906224333	25.19360678303316	27.59149691732889	24.21443939341361
44	22.03488103087298	24.98308290245341	28.140651929995027	24.84138413667858
45	22.15330071369909	23.43712044973453	27.807949011578813	26.601629824987565
46	22.385368590299706	25.33342587130282	27.683022752773244	24.598182785624225
47	20.9766225954587	25.429289424066816	29.525395889000706	24.068692091473785
48	21.469531873546867	23.22515066338157	30.119373980636432	25.185943482435135
49	21.59416895119779	23.21387259835051	30.13744780280159	25.054510647650112
50	19.583058611236424	23.674104405964073	30.619223606436012	26.12361337636349
51	19.72649246393911	24.13722802512406	27.820528391805766	28.315751119131065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	411.0
1	885.5
2	1360.0
3	2296.5
4	3233.0
5	2321.5
6	1410.0
7	1418.5
8	1427.0
9	1364.5
10	1302.0
11	1309.0
12	1316.0
13	1360.5
14	1405.0
15	1395.0
16	1385.0
17	1480.5
18	1576.0
19	1518.5
20	1461.0
21	1774.0
22	2087.0
23	2438.0
24	2789.0
25	3106.5
26	4411.5
27	5399.0
28	6921.0
29	8443.0
30	9353.5
31	10264.0
32	11745.0
33	13226.0
34	14519.0
35	15812.0
36	17105.5
37	18399.0
38	20850.5
39	23302.0
40	26707.0
41	30112.0
42	34184.0
43	38256.0
44	41687.0
45	45118.0
46	48926.0
47	52734.0
48	58735.5
49	64737.0
50	64632.0
51	64527.0
52	57975.0
53	51423.0
54	46837.5
55	42252.0
56	39057.5
57	35863.0
58	35622.0
59	35381.0
60	33412.5
61	31444.0
62	28641.0
63	25838.0
64	22886.5
65	19935.0
66	15874.5
67	11814.0
68	9915.0
69	8016.0
70	6981.0
71	5946.0
72	4735.0
73	3524.0
74	3065.0
75	1976.0
76	1346.0
77	1043.0
78	740.0
79	529.0
80	318.0
81	212.0
82	106.0
83	101.0
84	96.0
85	64.5
86	33.0
87	20.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	691608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.991873674208595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86995871296777	19.12912972876805
2	11.422236476721647	6.166151281132163
3	4.1721678821313075	3.37843885266236
4	2.1168083777762	2.2854649730176644
5	1.2808043913014922	1.7285655165690756
6	0.8917071361951103	1.4441307824741239
7	0.6378350400222346	1.2051453977684758
8	0.47130444698668195	1.0177112076125807
9	0.42901500091970557	1.0421926838248659
>10	6.961288979471241	47.197717014946626
>50	0.6720778003107986	10.910131455000158
>100	0.06937577293529953	3.2594632162810715
>500	0.004335985808456221	0.8040399388097292
>1k	0.0010839964521140552	0.4317179511330988
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1619	0.2340921446831153	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	1332	0.1925946489919145	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC	892	0.12897479497056136	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	870	0.1257938022694937	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	839	0.12131149437253474	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	782	0.11306983146522308	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07157233577402228	0.0
2	0.0	0.0	0.0	0.5322379151195475	0.0
3	0.0	0.0	0.0	0.8267689211229483	0.0
4	0.0	0.0	0.0	1.2969774785716763	0.0
5	0.0	0.0	0.0	2.612462551040474	0.0
6	0.0	0.0	0.0	3.4103133566991706	0.0
7	0.0	0.0	0.0	4.186330985182358	0.0
8	0.0	0.0	0.0	5.369804860556847	0.0
9	0.0	0.0	0.0	5.893078159882477	0.0
10	0.0	0.0	0.0	6.955674312616395	0.0
11	0.0	0.0	0.0	8.577546818428937	0.0
12	0.0	0.0	0.0	9.75451411782397	0.0
13	0.0	0.0	0.0	10.227759077396444	0.0
14	0.0	0.0	0.0	10.427149483522458	0.0
15	0.0	0.0	0.0	10.76664815907277	0.0
16	0.0	0.0	0.0	11.51316930978242	0.0
17	0.0	0.0	0.0	12.335745104163053	0.0
18	0.0	0.0	0.0	13.219916484482539	0.0
19	0.0	0.0	0.0	13.742322240344242	0.0
20	0.0	0.0	0.0	14.26660767371112	0.0
21	0.0	0.0	0.0	14.924205619368196	0.0
22	0.0	0.0	0.0	15.591346543128477	0.0
23	0.0	0.0	0.0	16.24171495991949	0.0
24	0.0	0.0	0.0	16.76021677019352	0.0
25	0.0	0.0	0.0	17.232305005147424	0.0
26	0.0	0.0	0.0	17.68140333830725	0.0
27	0.0	0.0	0.0	18.108379313136922	0.0
28	2.8918115464251424E-4	0.0	0.0	18.547500896461578	0.0
29	2.8918115464251424E-4	0.0	0.0	19.010479925044244	0.0
30	4.337717319637714E-4	0.0	0.0	19.54242865900915	0.0
31	5.783623092850285E-4	0.0	0.0	20.05413471214908	0.0
32	5.783623092850285E-4	0.0	0.0	20.510028802443003	0.0
33	5.783623092850285E-4	0.0	0.0	20.945391030757307	0.0
34	5.783623092850285E-4	0.0	0.0	21.38957328428821	0.0
35	5.783623092850285E-4	0.0	0.0	21.87134908792264	0.0
36	5.783623092850285E-4	0.0	0.0	22.346039953268324	0.0
37	5.783623092850285E-4	0.0	0.0	22.837503325583278	0.0
38	5.783623092850285E-4	0.0	0.0	23.27807081468115	0.0
39	5.783623092850285E-4	0.0	0.0	23.752906270604157	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCCG	25	3.8891885E-5	45.0	1
TAGCGAT	20	7.031336E-4	45.0	40
ATCCGGC	25	3.8891885E-5	45.0	35
TACGCTA	25	3.8891885E-5	45.0	39
CATACGT	20	7.031336E-4	45.0	34
ACGTCCA	25	3.8891885E-5	45.0	15
TACTACG	25	3.8891885E-5	45.0	36
TCGACAA	50	2.1827873E-11	45.0	19
TAAGACG	25	3.8891885E-5	45.0	16
AATCCGG	30	2.1642427E-6	44.999996	2
TACGTAG	30	2.1642427E-6	44.999996	1
CTTACGT	30	2.1642427E-6	44.999996	22
CTACGTA	90	0.0	42.5	21
TACGTAT	90	0.0	42.5	22
TCGATGG	65	0.0	41.53846	2
TTAATCG	100	0.0	40.5	20
CCCGGTT	50	1.0804797E-9	40.5	19
CCTACGT	95	0.0	40.263153	20
TACGAAT	95	0.0	40.263153	12
CGAATAT	95	0.0	40.263153	14
>>END_MODULE