##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549796_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426839
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31754361714839	33.0	31.0	34.0	30.0	34.0
2	32.42699706446693	34.0	31.0	34.0	30.0	34.0
3	32.44408547485117	34.0	31.0	34.0	30.0	34.0
4	35.954472295174526	37.0	35.0	37.0	35.0	37.0
5	35.99739714505938	37.0	35.0	37.0	35.0	37.0
6	36.018027874678744	37.0	35.0	37.0	35.0	37.0
7	36.15633529269818	37.0	36.0	37.0	35.0	37.0
8	35.78631287206652	37.0	35.0	37.0	35.0	37.0
9	37.678265106984135	39.0	38.0	39.0	35.0	39.0
10	37.60853155405199	39.0	37.0	39.0	35.0	39.0
11	37.51275539489128	39.0	37.0	39.0	35.0	39.0
12	37.29793200715024	39.0	37.0	39.0	35.0	39.0
13	37.16721293040233	39.0	37.0	39.0	34.0	39.0
14	38.40795709857815	40.0	38.0	41.0	34.0	41.0
15	38.473785197697495	40.0	38.0	41.0	34.0	41.0
16	38.57324658712067	40.0	38.0	41.0	35.0	41.0
17	38.57354412319399	40.0	38.0	41.0	35.0	41.0
18	38.56739894901825	40.0	37.0	41.0	35.0	41.0
19	38.56843446826555	40.0	37.0	41.0	35.0	41.0
20	38.45839063440782	40.0	37.0	41.0	35.0	41.0
21	38.414545062658284	40.0	37.0	41.0	34.0	41.0
22	38.38073606207493	40.0	37.0	41.0	34.0	41.0
23	38.34584702897345	40.0	37.0	41.0	34.0	41.0
24	38.29424209128032	40.0	37.0	41.0	34.0	41.0
25	38.23502069867093	40.0	36.0	41.0	34.0	41.0
26	38.21108661579659	40.0	36.0	41.0	34.0	41.0
27	38.16252263734101	40.0	36.0	41.0	34.0	41.0
28	38.1259866132195	40.0	36.0	41.0	34.0	41.0
29	38.12497217920574	40.0	36.0	41.0	34.0	41.0
30	38.04643202706407	40.0	36.0	41.0	34.0	41.0
31	37.981852642331184	40.0	36.0	41.0	34.0	41.0
32	37.87248119314308	40.0	36.0	41.0	34.0	41.0
33	37.826742635982185	40.0	36.0	41.0	34.0	41.0
34	37.751829144009804	40.0	36.0	41.0	34.0	41.0
35	37.685525455733895	40.0	36.0	41.0	34.0	41.0
36	37.63147697375357	40.0	35.0	41.0	34.0	41.0
37	37.53844423775709	40.0	35.0	41.0	33.0	41.0
38	37.4443923821394	40.0	35.0	41.0	33.0	41.0
39	37.41114331164678	40.0	35.0	41.0	33.0	41.0
40	37.32721471093316	40.0	35.0	41.0	33.0	41.0
41	37.28074754181319	40.0	35.0	41.0	33.0	41.0
42	37.25241835914713	40.0	35.0	41.0	33.0	41.0
43	37.168004798062036	39.0	35.0	41.0	33.0	41.0
44	37.1298053832944	39.0	35.0	41.0	33.0	41.0
45	37.06948755854081	39.0	35.0	41.0	33.0	41.0
46	36.95180384172955	39.0	35.0	41.0	33.0	41.0
47	36.889958508945995	39.0	35.0	41.0	32.0	41.0
48	36.88448103383243	39.0	35.0	41.0	33.0	41.0
49	36.80605333626965	39.0	35.0	41.0	32.0	41.0
50	36.72816214076033	39.0	35.0	41.0	32.0	41.0
51	35.946670758763844	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	8.0
13	8.0
14	11.0
15	16.0
16	18.0
17	36.0
18	87.0
19	122.0
20	217.0
21	433.0
22	690.0
23	1021.0
24	1402.0
25	1971.0
26	2443.0
27	3060.0
28	3290.0
29	3728.0
30	4648.0
31	5963.0
32	8074.0
33	11672.0
34	24180.0
35	41467.0
36	26810.0
37	40726.0
38	71551.0
39	172789.0
40	390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.179746930341416	21.27757772837065	23.475127624233025	12.067547717054907
2	31.063000335020934	26.573251272728125	23.60445038995968	18.75929800229126
3	31.982316517469116	25.82636544458215	26.328662563636406	15.862655474312328
4	29.50948718369221	23.930802949121333	27.765972650109294	18.793737217077165
5	26.321165591710223	29.468488118470898	24.28034926517961	19.929997024639267
6	25.407940698952064	34.739562223695586	24.16016343398799	15.692333643364359
7	76.17977738678987	6.717989686977995	11.731355382240142	5.3708775439919965
8	75.9089492759565	7.574050168799008	10.174562305693716	6.342438249550768
9	69.2019707664951	8.446510276708548	12.740635227802521	9.610883728993837
10	43.354520088370556	22.510829610227745	19.516023606090354	14.618626695311345
11	32.52069281391813	24.14844941535333	24.203505302936236	19.127352467792306
12	30.302760525631445	21.4380597836655	28.30060046059521	19.95857923010784
13	24.007881191737397	26.90639796269788	29.51721843599109	19.568502409573632
14	19.072062299836706	30.081131293063663	28.526915300616864	22.31989110648277
15	18.036308772159995	26.313668619784043	35.32807451990095	20.32194808815502
16	19.032703197224247	25.48736174529506	32.83954840115359	22.640386656327095
17	19.95201937967243	25.665180548169214	28.526915300616864	25.855884771541493
18	21.20495081283575	25.89828951899897	30.016001349454946	22.880758318710335
19	23.327530989436298	26.77262386989005	27.282417960870493	22.61742717980316
20	25.93624293937527	26.22112787257022	28.6396041598823	19.203025028172217
21	23.008909682573524	27.722865061533742	28.64428976733616	20.623935488556576
22	21.610958698713098	24.94359700027411	27.420409100386795	26.025035200625997
23	21.76253809984561	27.119593101848704	27.556525996921554	23.56134280138413
24	22.539411815696315	24.305182984685093	29.706985537872594	23.448419661745998
25	20.172477210376748	27.495378819648625	27.95269410714579	24.379449862828842
26	19.988098557067186	27.94426001372883	29.223196568261102	22.844444860942883
27	21.96495634185255	27.92903178950377	28.920272046368773	21.18573982227491
28	19.511103718263794	27.318262857892556	30.72188811237961	22.44874531146404
29	21.061336944374812	23.514955287590873	32.03971520877895	23.38399255925536
30	22.040394621859765	26.570205627883116	29.322062885537637	22.067336864719483
31	24.276366498843828	26.225110638906003	25.354993334723396	24.143529527526773
32	23.152054990289077	26.965670896989263	28.03984640578766	21.842427706933996
33	24.060125714847988	26.46782510501618	26.176848882131203	23.295200298004634
34	20.513355152645378	25.218642157815946	28.9261290556861	25.341873633852575
35	20.911397505851152	24.03786907944213	29.50081880990256	25.54991460480415
36	23.582193754553824	26.506949927255945	27.984087677086677	21.926768641103557
37	21.533646175724336	25.958031014035736	30.97608231675175	21.532240493488178
38	22.41055761071505	26.847827869524576	25.849793481851467	24.891821037908908
39	23.244361457130207	24.483001787559246	28.89473548574521	23.377901269565342
40	22.7713493846626	23.175951588303786	29.549080566677365	24.503618460356247
41	19.277526186688657	25.437928586656795	27.615564650840245	27.6689805758143
42	21.401746325898056	24.544617525577557	29.206328381427188	24.847307767097195
43	23.67004889431378	24.204910985172397	28.812034514184504	23.31300560632932
44	22.184711331438788	24.318302685555913	28.50114445962061	24.995841523384694
45	22.472173348733364	23.880901229737674	27.14442682135419	26.502498600174775
46	23.13823244830018	25.49204735274893	27.593308015434392	23.776412183516502
47	20.138740836708923	24.18851135908387	31.883918760937963	23.788829043269242
48	21.190425429728773	22.60360463781426	30.293389310723718	25.912580621733255
49	21.228378850105077	22.69263117943768	32.07462298431024	24.004366986147
50	21.01541799132694	23.19750538259156	30.42411775868653	25.362958867394962
51	20.14155220118124	23.095827700842705	27.05493171898538	29.70768837899067
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	217.0
1	485.0
2	753.0
3	1455.0
4	2157.0
5	1511.0
6	865.0
7	838.5
8	812.0
9	804.0
10	796.0
11	797.0
12	798.0
13	842.0
14	886.0
15	894.0
16	902.0
17	876.5
18	851.0
19	950.0
20	1049.0
21	1555.0
22	2061.0
23	1933.0
24	1805.0
25	2099.0
26	2915.0
27	3437.0
28	4028.0
29	4619.0
30	5805.5
31	6992.0
32	7784.0
33	8576.0
34	9989.5
35	11403.0
36	11559.0
37	11715.0
38	12305.5
39	12896.0
40	15486.5
41	18077.0
42	20798.5
43	23520.0
44	26890.5
45	30261.0
46	37777.0
47	45293.0
48	45727.0
49	46161.0
50	45077.5
51	43994.0
52	37560.5
53	31127.0
54	26823.5
55	22520.0
56	20203.0
57	17886.0
58	16435.0
59	14984.0
60	15020.0
61	15056.0
62	13550.5
63	12045.0
64	10816.5
65	9588.0
66	8097.5
67	6607.0
68	6049.5
69	5492.0
70	4394.5
71	3297.0
72	2690.0
73	2083.0
74	1811.5
75	1127.0
76	714.0
77	530.0
78	346.0
79	232.0
80	118.0
81	115.0
82	112.0
83	71.5
84	31.0
85	17.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426839.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.536342667142204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01278053989104	19.554322586930073
2	11.675778404889874	6.430164701253331
3	4.497814736796816	3.7156010353727753
4	2.182192185112769	2.4035836709930005
5	1.2823985495461776	1.765628294807484
6	0.7952156063689042	1.313839765874005
7	0.6141053112790344	1.1837149999564063
8	0.48598404679760343	1.0705778594706625
9	0.4296124861699371	1.0646960969932453
>10	6.343400462761693	44.32680295776571
>50	0.6021404738045424	10.031469178310775
>100	0.06918209699030912	3.686570214756791
>500	0.0034163998513732897	0.5844419128338518
>1k	0.005978699739903257	2.868586724681887
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	2136	0.5004228760727113	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTC	2067	0.48425753035687924	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCG	2030	0.4755891565672302	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGC	1959	0.4589552501060119	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	1529	0.35821468984792865	No Hit
CTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGCT	1266	0.29659895182961254	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1210	0.28347925095879245	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCC	728	0.1705561113206619	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	719	0.16844758796642295	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTGCCAT	531	0.12440287790009817	No Hit
ACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCTTCTGC	507	0.11878014895546096	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	482	0.11292313963813054	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTGCCATCGTATGCCGTCT	468	0.10964321442042549	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	465	0.10894037330234584	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	427	0.1000377191400036	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30573588636464805	0.0
2	0.0	0.0	0.0	1.4895546095834729	0.0
3	0.0	0.0	0.0	1.987634681929252	0.0
4	2.3428037269321688E-4	0.0	0.0	2.6656420805034218	0.0
5	2.3428037269321688E-4	0.0	0.0	4.787285135613193	0.0
6	2.3428037269321688E-4	0.0	0.0	5.683641841537441	0.0
7	2.3428037269321688E-4	0.0	0.0	6.703932864616401	0.0
8	2.3428037269321688E-4	0.0	0.0	8.182007735937907	0.0
9	2.3428037269321688E-4	0.0	0.0	8.742874948165468	0.0
10	2.3428037269321688E-4	0.0	0.0	10.440939089445903	0.0
11	2.3428037269321688E-4	0.0	0.0	12.291519753349624	0.0
12	2.3428037269321688E-4	0.0	0.0	14.039719894386408	0.0
13	2.3428037269321688E-4	0.0	0.0	14.595667218787412	0.0
14	2.3428037269321688E-4	0.0	0.0	14.81846785321866	0.0
15	2.3428037269321688E-4	0.0	0.0	15.313268000346735	0.0
16	2.3428037269321688E-4	0.0	0.0	16.148711809370745	0.0
17	2.3428037269321688E-4	0.0	0.0	17.10598141219523	0.0
18	2.3428037269321688E-4	0.0	0.0	18.175705593912458	0.0
19	2.3428037269321688E-4	0.0	0.0	18.887215085781758	0.0
20	2.3428037269321688E-4	0.0	0.0	19.474087419378268	0.0
21	2.3428037269321688E-4	0.0	0.0	20.191922481310282	0.0
22	2.3428037269321688E-4	0.0	0.0	20.940213991692417	0.0
23	2.3428037269321688E-4	0.0	0.0	21.673511558222188	0.0
24	2.3428037269321688E-4	0.0	0.0	22.26225813480024	0.0
25	2.3428037269321688E-4	0.0	0.0	22.80532003870312	0.0
26	2.3428037269321688E-4	0.0	0.0	23.30855427924815	0.0
27	2.3428037269321688E-4	0.0	0.0	23.79445177221388	0.0
28	2.3428037269321688E-4	0.0	0.0	24.27824074182537	0.0
29	2.3428037269321688E-4	0.0	0.0	24.7505499731749	0.0
30	2.3428037269321688E-4	0.0	0.0	25.336485185280633	0.0
31	2.3428037269321688E-4	0.0	0.0	25.8315196127814	0.0
32	2.3428037269321688E-4	0.0	0.0	26.37411295593889	0.0
33	2.3428037269321688E-4	0.0	0.0	26.815731458465603	0.0
34	2.3428037269321688E-4	0.0	0.0	27.282417960870493	0.0
35	2.3428037269321688E-4	0.0	0.0	27.78705788365168	0.0
36	2.3428037269321688E-4	0.0	0.0	28.272955376617414	0.0
37	2.3428037269321688E-4	0.0	0.0	28.778766701262068	0.0
38	4.6856074538643376E-4	0.0	0.0	29.206796942172577	0.0
39	4.6856074538643376E-4	0.0	0.0	29.68566602395751	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	60	0.0	45.000004	20
CGCGGGT	30	2.1622836E-6	45.000004	4
TGATCGG	20	7.028297E-4	45.0	21
TCGTTTG	20	7.028297E-4	45.0	1
TCGCACA	20	7.028297E-4	45.0	24
CGTATGG	20	7.028297E-4	45.0	2
AATCAGC	20	7.028297E-4	45.0	13
GGTCGCA	20	7.028297E-4	45.0	22
CATGCGG	45	3.8380676E-10	45.0	2
CCCTACG	25	3.8866703E-5	45.0	31
CCGGCAT	20	7.028297E-4	45.0	3
GACGTTA	20	7.028297E-4	45.0	10
TTTCGAC	25	3.8866703E-5	45.0	24
CGGCATT	20	7.028297E-4	45.0	4
TAGCGAT	20	7.028297E-4	45.0	33
CACGCCC	20	7.028297E-4	45.0	13
ATTCGAT	25	3.8866703E-5	45.0	14
ATAGACG	20	7.028297E-4	45.0	1
TACGGGT	35	1.209537E-7	45.0	4
TAGTTAG	25	3.8866703E-5	45.0	1
>>END_MODULE