FastQCFastQC Report
Sat 18 Jun 2016
SRR3549795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210116
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132431.0943578962677958No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC39090.32302688337316426No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG26520.2191525440536279No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC25140.20774867863907262No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC21240.17552036333706852Illumina Single End Adapter 1 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC17770.14684542638887513No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTT17710.14634960615345968No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCC17280.14279622779964896No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC17230.1423830442701361Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT16610.13725956850417645Illumina Single End Adapter 1 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTG15710.12982226497294475Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG600.045.0000041
CGAACGA207.033435E-445.022
TCCGATT207.033435E-445.010
ATTCGCG253.8909282E-545.01
TAACGCG253.8909282E-545.01
TCGATAG551.8189894E-1245.01
ATCGTAG351.2119926E-745.01
TCGCTCG253.8909282E-545.045
GTCCGAT207.033435E-445.09
ACGTCCC207.033435E-445.023
TCATCGA253.8909282E-545.016
CTCGACC253.8909282E-545.036
ACGTACG351.2119926E-745.01
TAGTCCG551.8189894E-1245.01
CGTTTTT67550.042.8349341
TACGTAG650.041.5384641
TTGTGCG603.6379788E-1241.2500041
TAATGCG451.9284926E-840.01
TACGGGA5200.039.3750044
TCGTTAG403.4584264E-739.3751
Produced by FastQC (version 0.11.5)