FastQCFastQC Report
Sat 18 Jun 2016
SRR3549794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731483
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG26420.36118406032676087No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC25700.3513410427856833No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC24880.3401309394750117No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC22450.3069107552738751TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15810.21613626017282697No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC14570.19918439662986015TruSeq Adapter, Index 21 (95% over 23bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC12780.17471356135412577No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT11120.15201993757886376Illumina Paired End PCR Primer 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG9610.13137694245799286No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9130.1248149307639412No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTT8130.11114407306800021No Hit
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC7450.10184788983476036TruSeq Adapter, Index 21 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG253.8894097E-545.01
GGTCGAC253.8894097E-545.011
GTTTACG253.8894097E-545.01
CACGCTA207.0316024E-445.038
TCGATTG207.0316024E-445.018
CGTTATC207.0316024E-445.019
ACTCGAC207.0316024E-445.035
CAATGCG207.0316024E-445.01
CCTATAG207.0316024E-445.01
TATCGCG207.0316024E-445.01
TAGTACG207.0316024E-445.01
ACGTAAC302.1644155E-644.99999626
CGCATGG1250.043.1999972
TATTGCG556.002665E-1140.909091
AACGGGC1550.040.645164
CCGCATG451.9266736E-840.01
CTCACGA403.4559162E-739.37524
CGGTCTA403.4559162E-739.37531
TAATGCG403.4559162E-739.3751
TACGGGC750.039.04
Produced by FastQC (version 0.11.5)