##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731483
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37643390208658	33.0	31.0	34.0	30.0	34.0
2	32.47037320074424	34.0	31.0	34.0	31.0	34.0
3	32.49000455239561	34.0	31.0	34.0	30.0	34.0
4	35.9807883436799	37.0	35.0	37.0	35.0	37.0
5	36.02319261008116	37.0	35.0	37.0	35.0	37.0
6	36.05410925476053	37.0	35.0	37.0	35.0	37.0
7	36.182555165328516	37.0	37.0	37.0	35.0	37.0
8	35.85285372318974	37.0	36.0	37.0	35.0	37.0
9	37.7397601858143	39.0	38.0	39.0	35.0	39.0
10	37.620380788070264	39.0	37.0	39.0	35.0	39.0
11	37.503780675695815	39.0	37.0	39.0	35.0	39.0
12	37.32036698050399	39.0	37.0	39.0	35.0	39.0
13	37.24166795400576	39.0	37.0	39.0	34.0	39.0
14	38.47565972141526	40.0	38.0	41.0	34.0	41.0
15	38.54962863115069	40.0	38.0	41.0	35.0	41.0
16	38.628061075923846	40.0	38.0	41.0	35.0	41.0
17	38.61964939718353	40.0	38.0	41.0	35.0	41.0
18	38.63254375016234	40.0	38.0	41.0	35.0	41.0
19	38.621613899434436	40.0	38.0	41.0	35.0	41.0
20	38.529043053632144	40.0	37.0	41.0	35.0	41.0
21	38.480961280029746	40.0	37.0	41.0	35.0	41.0
22	38.44550454350956	40.0	37.0	41.0	35.0	41.0
23	38.41790581599299	40.0	37.0	41.0	35.0	41.0
24	38.35969530392367	40.0	37.0	41.0	34.0	41.0
25	38.29259189892315	40.0	37.0	41.0	34.0	41.0
26	38.262922036465646	40.0	37.0	41.0	34.0	41.0
27	38.21353469595329	40.0	37.0	41.0	34.0	41.0
28	38.14785716141045	40.0	36.0	41.0	34.0	41.0
29	38.139615001305565	40.0	36.0	41.0	34.0	41.0
30	38.062350047779645	40.0	36.0	41.0	34.0	41.0
31	38.03206773089737	40.0	36.0	41.0	34.0	41.0
32	37.92912070410385	40.0	36.0	41.0	34.0	41.0
33	37.88297198977967	40.0	36.0	41.0	34.0	41.0
34	37.805525213846394	40.0	36.0	41.0	34.0	41.0
35	37.70145444255027	40.0	36.0	41.0	33.0	41.0
36	37.6287555554948	40.0	35.0	41.0	33.0	41.0
37	37.59211492269814	40.0	35.0	41.0	33.0	41.0
38	37.50604320264449	40.0	35.0	41.0	33.0	41.0
39	37.470694465900095	40.0	35.0	41.0	33.0	41.0
40	37.34038248325662	40.0	35.0	41.0	33.0	41.0
41	37.24337681121776	39.0	35.0	41.0	33.0	41.0
42	37.20088095006992	39.0	35.0	41.0	33.0	41.0
43	37.17335194392761	39.0	35.0	41.0	33.0	41.0
44	37.14857488143949	39.0	35.0	41.0	33.0	41.0
45	37.07877558330132	39.0	35.0	41.0	33.0	41.0
46	36.946173731993774	39.0	35.0	41.0	32.0	41.0
47	36.861627679658994	39.0	35.0	41.0	32.0	41.0
48	36.828251647680126	39.0	35.0	41.0	32.0	41.0
49	36.731159849237784	39.0	35.0	41.0	32.0	41.0
50	36.66445700036775	39.0	35.0	41.0	32.0	41.0
51	35.908753860308444	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	16.0
10	6.0
11	11.0
12	16.0
13	26.0
14	26.0
15	38.0
16	36.0
17	66.0
18	145.0
19	247.0
20	433.0
21	737.0
22	1241.0
23	1695.0
24	2320.0
25	3199.0
26	4049.0
27	4829.0
28	5530.0
29	6436.0
30	8075.0
31	10190.0
32	13401.0
33	19621.0
34	42060.0
35	65129.0
36	46817.0
37	71677.0
38	127005.0
39	295749.0
40	652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.984207425189645	20.23218584710786	23.53861948944815	12.24498723825434
2	31.717483523198766	25.399359930442678	24.836394010523826	18.046762535834734
3	31.2683958478871	25.623015162348274	25.967110650555103	17.141478339209524
4	28.64700888469042	24.76366504758142	27.33911792891974	19.250208138808418
5	27.344722980575074	28.93642094211349	23.414624810145966	20.30423126716547
6	26.569311932061307	34.71864691318869	22.958975123140252	15.753066031609759
7	78.80442881105918	6.212311154189503	9.897290846130396	5.085969188620925
8	79.03109163165787	6.934269149112146	8.464584959595781	5.570054259634196
9	73.3441515387234	7.814809093307705	10.296343182274912	8.544696185693994
10	45.0193647699263	21.581089375966357	18.09242320053918	15.307122653568161
11	31.30270970070391	25.039816373039432	23.31099970881073	20.346474217445927
12	29.81162925180763	21.289763398465855	27.279102863634563	21.61950448609195
13	25.190195807694778	24.155585297265965	29.383321280193798	21.270897614845456
14	20.44381072424103	27.761274014570404	28.528345839889646	23.26656942129892
15	19.933067480720673	25.744138961534308	32.8163470647985	21.50644649294652
16	22.629370744091112	24.420936645144177	30.348620542104193	22.601072068660518
17	21.716567575733134	25.59567344695639	27.640833758269164	25.046925219041317
18	22.876539851233726	25.05854544808287	28.550492629357073	23.514422071326333
19	23.99714005657001	26.16123682983747	26.681139548014105	23.16048356557842
20	25.813176792898812	25.756442733460656	27.892514248451434	20.537866225189102
21	25.33811448796486	26.694947114287004	27.01566543583378	20.95127296191436
22	23.161440525617135	23.77662912193448	27.634818580882946	25.427111771565436
23	23.105116591909862	25.84817418860042	27.56427695517189	23.482432264317833
24	23.382498294560502	24.85389270837463	28.670659468504393	23.09294952856047
25	22.90360814947169	26.583939749795967	26.812379781895135	23.70007231883721
26	21.31628486239598	27.153877807139743	27.30999900202739	24.21983832843689
27	22.629507452668072	26.86473916687059	28.341465215186133	22.1642881652752
28	20.740741753396865	25.844072931291635	29.62338154133452	23.791803773976973
29	21.450122559239244	24.201792796278244	29.626935964335466	24.721148680147042
30	22.220338681828558	25.267436153676847	29.044694135065342	23.467531029429257
31	24.13781318226124	24.99538608552762	25.7818705287751	25.084930203436034
32	23.68189007810161	26.382021181626914	26.14920647506504	23.786882265206437
33	24.33316973873624	24.542880695791975	26.363018689429556	24.76093087604223
34	22.911810664089256	24.788135882857155	27.77877271242121	24.52128074063239
35	22.90183093797122	23.168959497349903	29.636778981876542	24.292430582802336
36	23.287075707842835	24.877816709342525	28.809828799849075	23.025278782965565
37	22.295665107733196	25.2312083807826	29.395351634966225	23.077774876517978
38	22.3306625034348	25.345496751120667	27.08265263854389	25.241188106900637
39	24.245949666636136	23.345450270204502	28.138316269824454	24.270283793334908
40	24.57596417141615	22.659173213868264	29.76911288437325	22.995749730342332
41	21.430573232734048	23.65386481982493	30.062216073374227	24.853345874066793
42	21.303297547584837	23.661930625865534	30.42750139100977	24.607270435539856
43	22.760474269395186	22.915911921398035	28.92015262145532	25.403461187751457
44	22.53558866029696	23.439779188306495	28.118083400434458	25.906548750962088
45	23.240594791676635	23.63759649916676	26.73746348172138	26.384345227435226
46	23.76541901862381	24.825047198636195	27.9653799199708	23.444153862769195
47	21.41690237503811	23.564047284762598	31.513651034952282	23.505399305247014
48	21.86599005034977	22.449598965389487	30.89326751271048	24.79114347155026
49	22.130794563920144	22.17795902297114	31.53935224742065	24.151894165688063
50	21.779180103980543	22.226217150637815	30.67808821257637	25.31651453280527
51	21.492092092365784	22.66928964856326	28.275024846783865	27.563593412287098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	368.0
1	785.5
2	1203.0
3	2071.0
4	2939.0
5	2070.0
6	1201.0
7	1216.5
8	1232.0
9	1181.5
10	1131.0
11	1107.5
12	1084.0
13	1138.5
14	1193.0
15	1237.5
16	1282.0
17	1206.5
18	1131.0
19	1448.0
20	1765.0
21	1579.0
22	1393.0
23	1740.0
24	2087.0
25	2400.0
26	3855.5
27	4998.0
28	5975.5
29	6953.0
30	8532.5
31	10112.0
32	11565.0
33	13018.0
34	15297.5
35	17577.0
36	18200.5
37	18824.0
38	21012.0
39	23200.0
40	25903.5
41	28607.0
42	33794.5
43	38982.0
44	43325.5
45	47669.0
46	57907.0
47	68145.0
48	69010.5
49	69876.0
50	69525.5
51	69175.0
52	61715.0
53	54255.0
54	48956.0
55	43657.0
56	40990.5
57	38324.0
58	36974.0
59	35624.0
60	33497.5
61	31371.0
62	29374.0
63	27377.0
64	24600.5
65	21824.0
66	18370.5
67	14917.0
68	12565.0
69	10213.0
70	8568.0
71	6923.0
72	5576.0
73	4229.0
74	3201.5
75	1813.0
76	1452.0
77	1078.0
78	704.0
79	531.5
80	359.0
81	252.5
82	146.0
83	102.0
84	58.0
85	32.0
86	6.0
87	3.0
88	0.0
89	1.0
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	4.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731483.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.638540489130655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97343891084333	17.48681948257655
2	11.301063515326181	5.568834219852026
3	4.167602678482743	3.080509420092193
4	1.9843776282625765	1.955686741586902
5	1.1602105321558929	1.4292947086219394
6	0.7969483556477318	1.1781386597023644
7	0.5947769740082147	1.0258105589272808
8	0.45966376835685824	0.9060355494437461
9	0.3698529544273783	0.82013732934152
>10	7.197467329077487	47.045979242137086
>50	0.9160617473892732	13.55275897257591
>100	0.06956010819122346	3.028693882741
>500	0.004487748915562803	0.7965300436378578
>1k	0.004487748915562803	2.1247711887636282
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2642	0.36118406032676087	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	2570	0.3513410427856833	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	2488	0.3401309394750117	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	2245	0.3069107552738751	TruSeq Adapter, Index 21 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1581	0.21613626017282697	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1457	0.19918439662986015	TruSeq Adapter, Index 21 (95% over 23bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	1278	0.17471356135412577	No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	1112	0.15201993757886376	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	961	0.13137694245799286	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	913	0.1248149307639412	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTT	813	0.11114407306800021	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	745	0.10184788983476036	TruSeq Adapter, Index 21 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.101257308782296E-4	0.0	0.0	0.15830853211899662	0.0
2	4.101257308782296E-4	0.0	0.0	0.8639982063834702	0.0
3	4.101257308782296E-4	0.0	0.0	1.1613393612701868	0.0
4	4.101257308782296E-4	0.0	0.0	1.5878701213835456	0.0
5	4.101257308782296E-4	0.0	0.0	3.0253608081117402	0.0
6	4.101257308782296E-4	0.0	0.0	3.776847855657616	0.0
7	4.101257308782296E-4	0.0	0.0	4.492790673194046	0.0
8	4.101257308782296E-4	0.0	0.0	5.4598671466049105	0.0
9	4.101257308782296E-4	0.0	0.0	5.853177722517133	0.0
10	4.101257308782296E-4	0.0	0.0	7.075762526265136	0.0
11	4.101257308782296E-4	0.0	0.0	8.378731973265271	0.0
12	4.101257308782296E-4	0.0	0.0	9.624010400788535	0.0
13	4.101257308782296E-4	0.0	0.0	10.038374097552506	0.0
14	4.101257308782296E-4	0.0	0.0	10.223204093601629	0.0
15	5.468343078376394E-4	0.0	0.0	10.583704611043592	0.0
16	5.468343078376394E-4	0.0	0.0	11.233480477331668	0.0
17	5.468343078376394E-4	0.0	0.0	11.990709285109839	0.0
18	5.468343078376394E-4	0.0	0.0	12.778834231280836	0.0
19	5.468343078376394E-4	0.0	0.0	13.372149455284674	0.0
20	5.468343078376394E-4	0.0	0.0	13.866624378146861	0.0
21	5.468343078376394E-4	0.0	0.0	14.465818070959953	0.0
22	5.468343078376394E-4	0.0	0.0	15.11532052009411	0.0
23	5.468343078376394E-4	0.0	0.0	15.739668591067735	0.0
24	5.468343078376394E-4	0.0	0.0	16.278710510018687	0.0
25	5.468343078376394E-4	0.0	0.0	16.754456357837434	0.0
26	5.468343078376394E-4	0.0	0.0	17.184541540951738	0.0
27	5.468343078376394E-4	0.0	0.0	17.611482426795973	0.0
28	5.468343078376394E-4	0.0	0.0	18.038423312640212	0.0
29	5.468343078376394E-4	0.0	0.0	18.470969250139785	0.0
30	5.468343078376394E-4	0.0	0.0	18.959565704192716	0.0
31	5.468343078376394E-4	0.0	0.0	19.418769814199372	0.0
32	6.835428847970493E-4	0.0	0.0	19.87113849535806	0.0
33	6.835428847970493E-4	0.0	0.0	20.311476821744318	0.0
34	6.835428847970493E-4	0.0	0.0	20.745253136436528	0.0
35	8.202514617564592E-4	0.0	0.0	21.234533133374256	0.0
36	8.202514617564592E-4	0.0	0.0	21.674598042606593	0.0
37	8.202514617564592E-4	0.0	0.0	22.115073077569814	0.0
38	8.202514617564592E-4	0.0	0.0	22.547619015069387	0.0
39	8.202514617564592E-4	0.0	0.0	22.994245935995778	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	25	3.8894097E-5	45.0	1
GGTCGAC	25	3.8894097E-5	45.0	11
GTTTACG	25	3.8894097E-5	45.0	1
CACGCTA	20	7.0316024E-4	45.0	38
TCGATTG	20	7.0316024E-4	45.0	18
CGTTATC	20	7.0316024E-4	45.0	19
ACTCGAC	20	7.0316024E-4	45.0	35
CAATGCG	20	7.0316024E-4	45.0	1
CCTATAG	20	7.0316024E-4	45.0	1
TATCGCG	20	7.0316024E-4	45.0	1
TAGTACG	20	7.0316024E-4	45.0	1
ACGTAAC	30	2.1644155E-6	44.999996	26
CGCATGG	125	0.0	43.199997	2
TATTGCG	55	6.002665E-11	40.90909	1
AACGGGC	155	0.0	40.64516	4
CCGCATG	45	1.9266736E-8	40.0	1
CTCACGA	40	3.4559162E-7	39.375	24
CGGTCTA	40	3.4559162E-7	39.375	31
TAATGCG	40	3.4559162E-7	39.375	1
TACGGGC	75	0.0	39.0	4
>>END_MODULE