FastQCFastQC Report
Sat 18 Jun 2016
SRR3549791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788263
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47340.6005609802819617No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC15670.1987915200891073No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT14170.179762338204381No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC8750.1110035609942367No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC8340.10580225127907816No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCG207.031937E-445.045
GTCGCAA253.8896866E-545.032
ACACGTC207.031937E-445.011
CGTTCAC207.031937E-445.039
CACTCGA406.8102963E-945.044
GGGCGTA253.8896866E-545.07
TATAGCG207.031937E-445.01
ACGTACG253.8896866E-545.01
CGGTCCA207.031937E-445.020
TGTACCG207.031937E-445.01
GCGTACG502.1827873E-1145.01
ATCTCGA302.164632E-644.99999635
ACGTTAC302.164632E-644.99999634
TTATGCG302.164632E-644.9999961
CGAATAT1000.042.7514
TTAGGCG556.002665E-1140.9090921
TACGAAT1100.040.90909212
CGTTTTT29250.040.6923071
CGACGTT451.9270374E-840.027
AGTAACG451.9270374E-840.01