##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549791_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	788263
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42177293619008	33.0	31.0	34.0	31.0	34.0
2	32.53739297670955	34.0	31.0	34.0	31.0	34.0
3	32.5440430414722	34.0	31.0	34.0	31.0	34.0
4	36.034395880562705	37.0	35.0	37.0	35.0	37.0
5	36.083667506910764	37.0	35.0	37.0	35.0	37.0
6	36.12912695382125	37.0	36.0	37.0	35.0	37.0
7	36.303643580886074	37.0	37.0	37.0	35.0	37.0
8	36.0701263410816	37.0	37.0	37.0	35.0	37.0
9	37.95956171988283	39.0	38.0	39.0	35.0	39.0
10	37.728475140911094	39.0	38.0	39.0	35.0	39.0
11	37.65822193861693	39.0	37.0	39.0	35.0	39.0
12	37.54763194517566	39.0	37.0	39.0	35.0	39.0
13	37.535969593904575	39.0	37.0	39.0	35.0	39.0
14	38.84252463961901	40.0	38.0	41.0	35.0	41.0
15	38.912772767464666	40.0	38.0	41.0	35.0	41.0
16	38.95398490097848	40.0	38.0	41.0	35.0	41.0
17	38.97378920487198	40.0	38.0	41.0	35.0	41.0
18	38.94251537875049	40.0	38.0	41.0	35.0	41.0
19	38.86902214108743	40.0	38.0	41.0	35.0	41.0
20	38.77076559473171	40.0	38.0	41.0	35.0	41.0
21	38.73297998256927	40.0	38.0	41.0	35.0	41.0
22	38.72346158579053	40.0	38.0	41.0	35.0	41.0
23	38.70315744871952	40.0	38.0	41.0	35.0	41.0
24	38.654929890150875	40.0	38.0	41.0	35.0	41.0
25	38.58594022553386	40.0	38.0	41.0	35.0	41.0
26	38.572086981121785	40.0	38.0	41.0	35.0	41.0
27	38.52406747494174	40.0	38.0	41.0	35.0	41.0
28	38.47897719415982	40.0	37.0	41.0	35.0	41.0
29	38.461481764335	40.0	37.0	41.0	35.0	41.0
30	38.37944061816932	40.0	37.0	41.0	35.0	41.0
31	38.29016077121469	40.0	37.0	41.0	34.0	41.0
32	38.14338488550141	40.0	37.0	41.0	34.0	41.0
33	38.025430598670745	40.0	37.0	41.0	34.0	41.0
34	37.90093915355662	40.0	37.0	41.0	34.0	41.0
35	37.79984725910007	40.0	37.0	41.0	34.0	41.0
36	37.714177882255036	40.0	37.0	41.0	34.0	41.0
37	37.65428924102742	40.0	37.0	41.0	33.0	41.0
38	37.591251904503956	40.0	37.0	41.0	33.0	41.0
39	37.541073220486055	40.0	36.0	41.0	33.0	41.0
40	37.47535150070471	40.0	36.0	41.0	33.0	41.0
41	37.414228499878845	40.0	36.0	41.0	33.0	41.0
42	37.38721594188742	40.0	36.0	41.0	33.0	41.0
43	37.33119022458241	40.0	36.0	41.0	33.0	41.0
44	37.26791692620356	40.0	35.0	41.0	33.0	41.0
45	37.21740079136024	40.0	35.0	41.0	33.0	41.0
46	37.101642472119075	40.0	35.0	41.0	33.0	41.0
47	37.060417652484006	40.0	35.0	41.0	32.0	41.0
48	37.00616038048215	40.0	35.0	41.0	32.0	41.0
49	36.92427527360792	39.0	35.0	41.0	32.0	41.0
50	36.87377816794648	39.0	35.0	41.0	32.0	41.0
51	36.149703842499264	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	6.0
12	9.0
13	13.0
14	24.0
15	27.0
16	38.0
17	50.0
18	108.0
19	196.0
20	323.0
21	568.0
22	857.0
23	1422.0
24	2538.0
25	4156.0
26	6380.0
27	7347.0
28	7291.0
29	7264.0
30	7873.0
31	9686.0
32	12336.0
33	17244.0
34	34248.0
35	56704.0
36	45019.0
37	68573.0
38	134448.0
39	362698.0
40	810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.48039803974054	21.500692027914543	25.55289287966072	12.466017052684194
2	34.02265487533983	23.403483355174608	25.644867258770233	16.92899451071533
3	32.123161939606454	23.015795489576448	28.63219509224713	16.228847478569968
4	28.103691280701998	25.57801139974856	28.137563224456812	18.180734095092628
5	25.042403360299797	30.08919611855434	26.043084605011273	18.82531591613459
6	24.97554750127813	34.092682264675624	25.639412226629943	15.292358007416308
7	81.5369997069506	5.077239449270103	9.329627294443606	4.056133549335692
8	83.10132531908766	4.376077527424223	8.790594002255592	3.73200315123252
9	77.25835159077617	7.224619194355183	10.596590224328683	4.920438990539959
10	38.70573653717097	29.529484448718257	18.314826396773665	13.449952617337107
11	27.542203553890012	25.58854088039144	28.00943339976632	18.859822165952224
12	26.31126920837335	22.81459360644861	31.114234716078265	19.75990246909978
13	23.34411230769426	25.046336057889306	32.269179195268585	19.34037243914785
14	20.252123973851365	28.183233260980156	30.688995931560914	20.875646833607565
15	19.37995313746808	27.05251927338972	34.80805264232877	18.759474946813437
16	22.08831316451489	26.305433592595367	32.76812434428611	18.83812889860364
17	22.10176045304676	25.70233538806211	30.010161583126443	22.18574257576469
18	22.614533474233852	25.831733824878246	31.618888619661206	19.934844081226696
19	23.300852634209647	27.818760997281366	29.48178463279388	19.39860173571511
20	25.05369400821807	27.08448829895606	29.508932932282754	18.352884760543116
21	23.233743052762847	27.108084484493123	30.237750598467773	19.420421864276264
22	22.14755735078267	25.38670469120078	29.917299175528978	22.54843878248757
23	21.226291225136787	27.27731734205462	29.824056184293823	21.672335248514774
24	21.34110062250797	25.971534881124704	31.393709967358607	21.29365452900872
25	21.139264433317308	27.75786761525024	29.34071496442177	21.76215298701068
26	20.61583507027477	29.059209933740387	29.77610264594431	20.548852350040534
27	21.371420452310968	27.67769132890926	30.523695771588926	20.427192447190848
28	19.486262833597415	27.9810164881518	31.528817158740168	21.00390351951062
29	20.388246054933443	26.403624171120555	31.1833740769261	22.024755697019906
30	21.82317323025437	26.98160385556597	29.751745293131858	21.443477621047798
31	22.171280397532296	28.415643002398944	27.73769668245243	21.67537991761633
32	21.441574702859327	28.99248093593128	28.279901504954562	21.286042856254827
33	22.992706748889645	28.381517336219	27.11569615724701	21.51007975764434
34	20.706033392408372	27.804552541474102	28.39217367807445	23.097240388043076
35	20.902160827033615	27.14868007251387	28.303117106853932	23.646041993598583
36	22.572161829237196	28.80066678253324	27.334278026496232	21.29289336173333
37	21.369136950484798	29.864778633527134	27.929637697063036	20.83644671892503
38	21.369010089272237	29.456539251493474	26.74957469778488	22.424875961449413
39	22.62760017912803	27.876609710210932	27.748479885520442	21.747310225140595
40	22.774124879640425	25.381376520273058	28.958735853389033	22.885762746697484
41	19.897927468370327	26.718493700706492	28.46714865470027	24.91643017622291
42	20.913451474951888	27.760531700714104	28.534131374934507	22.7918854493995
43	21.583405538506817	27.8160969118175	28.585129582385576	22.015367967290107
44	21.227940420900133	27.394283380039404	28.1754947270137	23.202281472046764
45	21.670051746688603	26.80729654950188	27.764591259516173	23.75806044429334
46	22.49160495925852	27.46075865542338	27.018393607209777	23.029242778108323
47	20.68192976202105	27.162888528321133	29.244934748935318	22.9102469607225
48	20.591604578674886	26.327253721156517	29.27918727632782	23.801954423840773
49	21.16501725946797	26.381169736496574	29.537476705109846	22.916336298925614
50	20.50089881169102	25.802936329626025	29.32549161891399	24.370673239768962
51	19.79504302498024	26.22513044504182	27.643819385154444	26.33600714482349
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	230.0
1	589.5
2	949.0
3	4499.0
4	8049.0
5	5515.5
6	2982.0
7	2895.5
8	2809.0
9	2815.5
10	2822.0
11	2803.0
12	2784.0
13	2760.0
14	2736.0
15	2692.0
16	2648.0
17	2587.0
18	2526.0
19	2537.0
20	2548.0
21	2842.5
22	3137.0
23	3389.5
24	3642.0
25	4286.0
26	6362.0
27	7794.0
28	9114.5
29	10435.0
30	12663.0
31	14891.0
32	16098.0
33	17305.0
34	20396.0
35	23487.0
36	26171.5
37	28856.0
38	30470.0
39	32084.0
40	36582.5
41	41081.0
42	44370.0
43	47659.0
44	52857.5
45	58056.0
46	62871.0
47	67686.0
48	73270.5
49	78855.0
50	76736.0
51	74617.0
52	66352.0
53	58087.0
54	50714.0
55	43341.0
56	38220.0
57	33099.0
58	30675.5
59	28252.0
60	26446.5
61	24641.0
62	22080.5
63	19520.0
64	16892.5
65	14265.0
66	10897.5
67	7530.0
68	6504.0
69	5478.0
70	4637.5
71	3797.0
72	2946.0
73	2095.0
74	1749.0
75	1043.0
76	683.0
77	461.5
78	240.0
79	203.0
80	166.0
81	102.0
82	38.0
83	32.0
84	26.0
85	14.0
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	788263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.89330077600854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9684746782032	22.542370354002948
2	10.671737973990314	6.593704220664696
3	3.8478098915117167	3.5661464492211685
4	1.8899755009613404	2.3355032644194447
5	1.2751094579493698	1.969617000338155
6	0.896786816768258	1.6622822917428635
7	0.6497848691403696	1.4051799581436952
8	0.6227892828025761	1.53920133069557
9	0.4748103859595439	1.3201614058518802
>10	6.1568387617155045	44.18188614415548
>50	0.506355836745678	9.472564648915192
>100	0.036197993157212616	1.954796971648794
>500	0.002080344434322564	0.4645342093331629
>1k	0.0012482066605935384	0.9920517508670037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4734	0.6005609802819617	No Hit
GCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	1567	0.1987915200891073	No Hit
CTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGCT	1417	0.179762338204381	No Hit
CCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCCGTCTTCTGC	875	0.1110035609942367	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAAGCCATCGTATGCC	834	0.10580225127907816	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18547109276979892	0.0
2	0.0	0.0	0.0	0.6534621059215008	0.0
3	0.0	0.0	0.0	0.9112440898532597	0.0
4	0.0	0.0	0.0	1.2310612067292261	0.0
5	0.0	0.0	0.0	2.1459842717468662	0.0
6	0.0	0.0	0.0	2.8192367268284824	0.0
7	0.0	0.0	0.0	3.427916824714594	0.0
8	0.0	0.0	0.0	4.389271093530966	0.0
9	1.2686121256484193E-4	0.0	0.0	4.802584924067221	0.0
10	1.2686121256484193E-4	0.0	0.0	5.785632460232182	0.0
11	1.2686121256484193E-4	0.0	0.0	7.221574525253628	0.0
12	1.2686121256484193E-4	0.0	0.0	8.33833377946193	0.0
13	1.2686121256484193E-4	0.0	0.0	8.833853675740203	0.0
14	1.2686121256484193E-4	0.0	0.0	9.04545817829836	0.0
15	1.2686121256484193E-4	0.0	0.0	9.38214783644545	0.0
16	1.2686121256484193E-4	0.0	0.0	10.046266284222398	0.0
17	1.2686121256484193E-4	0.0	0.0	10.915138729079	0.0
18	1.2686121256484193E-4	0.0	0.0	11.818644284965805	0.0
19	1.2686121256484193E-4	0.0	0.0	12.39332557788454	0.0
20	1.2686121256484193E-4	0.0	0.0	12.927284421569958	0.0
21	1.2686121256484193E-4	0.0	0.0	13.625274813101719	0.0
22	1.2686121256484193E-4	0.0	0.0	14.370330714495035	0.0
23	1.2686121256484193E-4	0.0	0.0	15.154206146933193	0.0
24	1.2686121256484193E-4	0.0	0.0	15.730917219252966	0.0
25	1.2686121256484193E-4	0.0	0.0	16.25447344350807	0.0
26	1.2686121256484193E-4	0.0	0.0	16.734643133065994	0.0
27	1.2686121256484193E-4	0.0	0.0	17.20187297894231	0.0
28	1.2686121256484193E-4	0.0	0.0	17.694855650969284	0.0
29	1.2686121256484193E-4	0.0	0.0	18.17629395265286	0.0
30	1.2686121256484193E-4	0.0	0.0	18.74463218494335	0.0
31	1.2686121256484193E-4	0.0	0.0	19.2856952565324	0.0
32	1.2686121256484193E-4	0.0	1.2686121256484193E-4	19.823206214169637	0.0
33	1.2686121256484193E-4	0.0	1.2686121256484193E-4	20.318345526810216	0.0
34	1.2686121256484193E-4	0.0	1.2686121256484193E-4	20.813738561875923	0.0
35	1.2686121256484193E-4	0.0	1.2686121256484193E-4	21.33716792491846	0.0
36	1.2686121256484193E-4	0.0	1.2686121256484193E-4	21.850321529743244	0.0
37	1.2686121256484193E-4	0.0	1.2686121256484193E-4	22.406861669265208	0.0
38	1.2686121256484193E-4	0.0	1.2686121256484193E-4	22.92052271894025	0.0
39	2.5372242512968385E-4	0.0	1.2686121256484193E-4	23.42872873647501	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCG	20	7.031937E-4	45.0	45
GTCGCAA	25	3.8896866E-5	45.0	32
ACACGTC	20	7.031937E-4	45.0	11
CGTTCAC	20	7.031937E-4	45.0	39
CACTCGA	40	6.8102963E-9	45.0	44
GGGCGTA	25	3.8896866E-5	45.0	7
TATAGCG	20	7.031937E-4	45.0	1
ACGTACG	25	3.8896866E-5	45.0	1
CGGTCCA	20	7.031937E-4	45.0	20
TGTACCG	20	7.031937E-4	45.0	1
GCGTACG	50	2.1827873E-11	45.0	1
ATCTCGA	30	2.164632E-6	44.999996	35
ACGTTAC	30	2.164632E-6	44.999996	34
TTATGCG	30	2.164632E-6	44.999996	1
CGAATAT	100	0.0	42.75	14
TTAGGCG	55	6.002665E-11	40.909092	1
TACGAAT	110	0.0	40.909092	12
CGTTTTT	2925	0.0	40.692307	1
CGACGTT	45	1.9270374E-8	40.0	27
AGTAACG	45	1.9270374E-8	40.0	1
>>END_MODULE