SRR3549789_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734997
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	5044	0.6862613044679093	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	4880	0.6639482882243056	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	4326	0.5885738309135955	No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1971	0.26816435985453	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1680	0.22857236151984295	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACCAGAT	1248	0.16979661141474045	No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1030	0.14013662640799895	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT	944	0.1284358983778165	No Hit
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	892	0.12136103956886898	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTA	781	0.10625893711130793	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCC	751	0.1021772877984536	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	735	0.10000040816493128	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.0316234E-4	45.000004	19
GTTTACG	20	7.0316234E-4	45.000004	1
TCGATTG	20	7.0316234E-4	45.000004	1
TACGGGT	85	0.0	45.000004	4
AAACGAT	20	7.0316234E-4	45.000004	16
TATCTCG	20	7.0316234E-4	45.000004	26
TGTAGCG	30	2.16443E-6	45.000004	1
GACGCGT	20	7.0316234E-4	45.000004	39
ATCGAAT	80	0.0	45.000004	43
TAGCGCG	25	3.8894286E-5	45.0	1
CGATCGA	25	3.8894286E-5	45.0	41
GGTTACG	25	3.8894286E-5	45.0	1
CGAATAT	55	6.002665E-11	40.909092	14
GATAGCG	85	0.0	39.705883	1
TACGGCT	625	0.0	39.600002	7
CGGGTAT	80	0.0	39.375004	6
ATCACGG	40	3.4559525E-7	39.375004	2
CGGAAAG	35	6.2452145E-6	38.57143	7
CGTATGG	35	6.2452145E-6	38.57143	2
CCGATCG	35	6.2452145E-6	38.57143	40