##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734997
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.308474728468276	33.0	31.0	34.0	30.0	34.0
2	32.415492852351775	34.0	31.0	34.0	30.0	34.0
3	32.4483855036143	34.0	31.0	34.0	30.0	34.0
4	35.95457804589679	37.0	35.0	37.0	35.0	37.0
5	35.976451604564375	37.0	35.0	37.0	35.0	37.0
6	36.00278096373182	37.0	35.0	37.0	35.0	37.0
7	36.16035847765365	37.0	36.0	37.0	35.0	37.0
8	35.81304277432425	37.0	35.0	37.0	35.0	37.0
9	37.73852682391901	39.0	38.0	39.0	35.0	39.0
10	37.602198376319905	39.0	37.0	39.0	35.0	39.0
11	37.49824421052059	39.0	37.0	39.0	35.0	39.0
12	37.246266311291066	39.0	37.0	39.0	35.0	39.0
13	37.110947391622005	39.0	37.0	39.0	33.0	39.0
14	38.19680080326859	40.0	37.0	41.0	33.0	41.0
15	38.27783922927576	40.0	37.0	41.0	33.0	41.0
16	38.45617329050323	40.0	37.0	41.0	34.0	41.0
17	38.45384947149444	40.0	37.0	41.0	34.0	41.0
18	38.47348764688835	40.0	37.0	41.0	34.0	41.0
19	38.45343042216499	40.0	37.0	41.0	34.0	41.0
20	38.32590881323325	40.0	37.0	41.0	34.0	41.0
21	38.30936588856825	40.0	37.0	41.0	34.0	41.0
22	38.30892643099224	40.0	37.0	41.0	34.0	41.0
23	38.250179252432325	40.0	37.0	41.0	34.0	41.0
24	38.20174095948691	40.0	36.0	41.0	34.0	41.0
25	38.154659134663135	40.0	36.0	41.0	34.0	41.0
26	38.11599639182201	40.0	36.0	41.0	34.0	41.0
27	38.043592014661286	40.0	36.0	41.0	34.0	41.0
28	37.99453603211986	40.0	36.0	41.0	34.0	41.0
29	37.95904609134459	40.0	36.0	41.0	34.0	41.0
30	37.87924984727829	40.0	36.0	41.0	34.0	41.0
31	37.79849577617323	40.0	36.0	41.0	33.0	41.0
32	37.670624505950364	40.0	35.0	41.0	33.0	41.0
33	37.61693176978954	40.0	35.0	41.0	33.0	41.0
34	37.502963957676016	40.0	35.0	41.0	33.0	41.0
35	37.40393906369686	40.0	35.0	41.0	33.0	41.0
36	37.384515855166754	40.0	35.0	41.0	33.0	41.0
37	37.29551957354928	40.0	35.0	41.0	33.0	41.0
38	37.233704355255874	40.0	35.0	41.0	33.0	41.0
39	37.1691381053256	40.0	35.0	41.0	33.0	41.0
40	37.06167236056746	39.0	35.0	41.0	32.0	41.0
41	36.97057402955386	39.0	35.0	41.0	32.0	41.0
42	36.943177999365986	39.0	35.0	41.0	32.0	41.0
43	36.87365934826945	39.0	35.0	41.0	32.0	41.0
44	36.822569343820454	39.0	35.0	41.0	32.0	41.0
45	36.730355361994675	39.0	35.0	41.0	32.0	41.0
46	36.56913837743556	39.0	35.0	41.0	31.0	41.0
47	36.45507940848738	38.0	35.0	41.0	31.0	41.0
48	36.43782899794149	38.0	35.0	41.0	31.0	41.0
49	36.34132384213813	38.0	35.0	41.0	31.0	41.0
50	36.23245673111591	38.0	35.0	40.0	31.0	41.0
51	35.41531598088155	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	13.0
11	4.0
12	11.0
13	17.0
14	21.0
15	25.0
16	54.0
17	87.0
18	157.0
19	284.0
20	545.0
21	996.0
22	1700.0
23	2345.0
24	2990.0
25	3824.0
26	4932.0
27	5633.0
28	6247.0
29	7092.0
30	9001.0
31	11425.0
32	15408.0
33	22323.0
34	47425.0
35	63364.0
36	47065.0
37	72543.0
38	132175.0
39	276801.0
40	477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.01869123275333	19.37436479332569	23.85234225445818	10.7546017194628
2	32.24734250615989	26.63099305167232	23.388258727586646	17.73340571458115
3	31.679857196696044	26.826776163712236	24.252752052049193	17.240614587542535
4	27.708548470265864	25.237381921286755	28.00542043028747	19.04864917815991
5	27.892086634367214	28.432905168320417	24.050846466039996	19.624161731272373
6	25.5218728783927	35.73075808472688	23.5743819362528	15.172987100627623
7	78.3616803878111	5.854173554449882	11.20099809931197	4.583147958427041
8	78.36086405794853	7.610507253771104	8.818947560330178	5.209681127950183
9	73.37526547727406	6.632816188365395	10.281538564102982	9.710379770257566
10	45.04467365172919	22.598459585549328	17.648915573805063	14.707951188916418
11	34.95796581482645	23.939689549753265	21.96063385292729	19.14171078249299
12	29.715359382419248	21.758864321895192	27.029225969629806	21.496550326055754
13	27.507323159142143	23.433973199890612	29.887468928444605	19.171234712522637
14	22.19941033772927	27.274124928401065	29.14188765396321	21.38457707990645
15	19.708515817071362	23.999281629720937	34.86367971569952	21.42852283750818
16	24.6475835955793	24.72023695334811	28.40202068851982	22.230158762552772
17	24.432344621814785	25.460784193676982	26.954531787204573	23.152339397303663
18	26.48691083092856	23.6949266459591	28.146781551489326	21.671380971623012
19	25.16404828863247	25.831397951284156	26.010582356118462	22.993971403964913
20	26.889225398198903	26.816435985453	27.599568433612653	18.69477018273544
21	24.759012621820226	27.590452750147282	28.38732675099354	19.263207877038955
22	23.631388971655667	22.95628417530956	29.66297821623762	23.74934863679716
23	23.16050269592937	26.406774449419522	28.85168238781927	21.581040466831837
24	23.474517583064966	24.790033156597918	28.356442271192943	23.379006989144173
25	23.217237621378047	27.381880470260423	26.36146814204684	23.03941376631469
26	22.441588196958627	25.150714900877148	27.937801106671184	24.469895795493045
27	23.960369906271726	26.105684785107968	27.600384763475226	22.333560545145083
28	19.994231268971166	27.335621778048075	28.321884307010777	24.348262645969985
29	23.551524700100817	26.016704830087743	27.10936235113885	23.322408118672595
30	24.118737899610473	26.912490799282175	27.045552566881227	21.923218734226126
31	24.90064585297627	26.758612620187566	24.607311322359138	23.733430204477024
32	26.739020703485867	26.725007040845068	24.30826248270401	22.22770977296506
33	24.463637266546666	26.65153735321369	23.290435199055235	25.594390181184412
34	22.998461218209055	27.063375768880686	26.544734196193996	23.39342881671626
35	23.302135927085416	27.83752858855206	25.77071743149972	23.0896180528628
36	25.05534036193345	30.39930775227654	23.21410835690486	21.331243528885153
37	22.14417201702864	30.283797076722763	24.741053364843665	22.83097754140493
38	22.338458524320508	28.873315129177396	23.231795503927227	25.55643084257487
39	22.91505951724973	26.312488350292583	24.52404567637691	26.248406456080776
40	25.153163890464857	24.05805737982604	26.360923922138458	24.427854807570643
41	22.0892058062822	27.194260656846218	24.816427822154377	25.900105714717203
42	23.708532143668613	25.322008117039935	27.286097766385442	23.68336197290601
43	22.69900421362264	25.80309851604837	25.905003693892624	25.592893576436364
44	22.806215535573614	24.374929421480633	27.253172461928415	25.565682581017334
45	22.967576738408457	24.02363547062097	25.692213709715823	27.316574081254753
46	24.579147942100445	25.823778872566827	25.676975552281167	23.92009763305156
47	20.94430317402656	26.607319485657765	29.03100284763067	23.417374492685003
48	22.08811736646544	26.90228667600004	26.682557887991383	24.327038069543143
49	23.882274349419113	23.759824870033484	28.14623733158095	24.21166344896646
50	21.32757004450358	25.11085079258827	27.417390819282257	26.144188343625892
51	21.512468758375885	23.98798906662204	25.597111280726313	28.90243089427576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	353.0
1	748.0
2	1143.0
3	2111.5
4	3080.0
5	2173.0
6	1266.0
7	1212.0
8	1158.0
9	1136.5
10	1115.0
11	1142.0
12	1169.0
13	1172.0
14	1175.0
15	1166.0
16	1157.0
17	1162.5
18	1168.0
19	1317.5
20	1467.0
21	1749.5
22	2032.0
23	2154.5
24	2277.0
25	2960.5
26	4209.5
27	4775.0
28	5812.5
29	6850.0
30	7920.0
31	8990.0
32	10814.5
33	12639.0
34	13899.0
35	15159.0
36	17172.0
37	19185.0
38	20132.0
39	21079.0
40	23922.0
41	26765.0
42	30930.5
43	35096.0
44	38318.5
45	41541.0
46	55567.0
47	69593.0
48	68381.5
49	67170.0
50	69824.5
51	72479.0
52	65584.0
53	58689.0
54	54118.5
55	49548.0
56	47468.0
57	45388.0
58	42639.0
59	39890.0
60	36228.0
61	32566.0
62	30220.0
63	27874.0
64	23724.0
65	19574.0
66	16532.0
67	13490.0
68	11697.5
69	9905.0
70	7557.0
71	5209.0
72	4437.5
73	3666.0
74	3208.0
75	2153.0
76	1556.0
77	1228.0
78	900.0
79	626.0
80	352.0
81	216.5
82	81.0
83	44.0
84	7.0
85	7.0
86	7.0
87	11.0
88	15.0
89	9.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	734997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.775178166651344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.32149560549382	19.09645751949138
2	11.139116536151187	5.965036597490804
3	4.221282420145544	3.390767666734503
4	2.2015839096522947	2.357912057190921
5	1.2741539147351961	1.7057849039385582
6	0.8383402985022251	1.346802651400845
7	0.6446734315946299	1.2082872193176915
8	0.5186577588474126	1.110972312052445
9	0.41302769920582666	0.995300121059828
>10	6.648005995963528	45.06202121950022
>50	0.7117314457338799	11.334830985309207
>100	0.05969692531092261	2.7072140088509125
>500	0.004631657998261237	0.9380929898843087
>1k	0.0030877719988408243	2.0854931553905716
>5k	5.146286664734708E-4	0.6950265923878456
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	5044	0.6862613044679093	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	4880	0.6639482882243056	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	4326	0.5885738309135955	No Hit
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1971	0.26816435985453	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1680	0.22857236151984295	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACCAGAT	1248	0.16979661141474045	No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1030	0.14013662640799895	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCT	944	0.1284358983778165	No Hit
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	892	0.12136103956886898	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACCAGATCGTA	781	0.10625893711130793	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCC	751	0.1021772877984536	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	735	0.10000040816493128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.360549770951446E-4	0.0	0.0	0.1321093827593854	0.0
2	1.360549770951446E-4	0.0	0.0	0.6836762599031017	0.0
3	1.360549770951446E-4	0.0	0.0	0.9186432053464164	0.0
4	1.360549770951446E-4	0.0	0.0	1.342590513974887	0.0
5	1.360549770951446E-4	0.0	0.0	2.933889526079698	0.0
6	1.360549770951446E-4	0.0	0.0	3.581783327006777	0.0
7	1.360549770951446E-4	0.0	0.0	4.32287478724403	0.0
8	1.360549770951446E-4	0.0	0.0	5.426144596508557	0.0
9	1.360549770951446E-4	0.0	0.0	5.860976303304639	0.0
10	1.360549770951446E-4	0.0	0.0	7.463567878508347	0.0
11	1.360549770951446E-4	0.0	0.0	8.809967931841898	0.0
12	1.360549770951446E-4	0.0	0.0	10.532559996843524	0.0
13	1.360549770951446E-4	0.0	0.0	10.991609489562542	0.0
14	1.360549770951446E-4	0.0	0.0	11.19487562534269	0.0
15	1.360549770951446E-4	0.0	0.0	11.628074672413629	0.0
16	1.360549770951446E-4	0.0	0.0	12.301818918988785	0.0
17	1.360549770951446E-4	0.0	0.0	13.038012400050613	0.0
18	1.360549770951446E-4	0.0	0.0	13.809308065202988	0.0
19	1.360549770951446E-4	0.0	0.0	14.486589741182618	0.0
20	1.360549770951446E-4	0.0	0.0	15.029449099792243	0.0
21	1.360549770951446E-4	0.0	0.0	15.648907410506437	0.0
22	1.360549770951446E-4	0.0	0.0	16.3029236854028	0.0
23	1.360549770951446E-4	0.0	0.0	16.872449819523073	0.0
24	1.360549770951446E-4	0.0	0.0	17.364560671676212	0.0
25	2.721099541902892E-4	0.0	0.0	17.822249614624276	0.0
26	2.721099541902892E-4	0.0	0.0	18.255040496763932	0.0
27	4.0816493128543384E-4	0.0	0.0	18.74687923896288	0.0
28	4.0816493128543384E-4	0.0	0.0	19.167833338095257	0.0
29	4.0816493128543384E-4	0.0	0.0	19.635318239394174	0.0
30	4.0816493128543384E-4	0.0	0.0	20.201579054064165	0.0
31	4.0816493128543384E-4	0.0	0.0	20.673009549698843	0.0
32	4.0816493128543384E-4	0.0	0.0	21.10661676170107	0.0
33	4.0816493128543384E-4	0.0	0.0	21.554237636344094	0.0
34	4.0816493128543384E-4	0.0	0.0	22.028797396451957	0.0
35	4.0816493128543384E-4	0.0	0.0	22.538595395627464	0.0
36	5.442199083805784E-4	0.0	0.0	22.982950950820207	0.0
37	5.442199083805784E-4	0.0	0.0	23.471388318591778	0.0
38	5.442199083805784E-4	0.0	0.0	24.015064007063973	0.0
39	5.442199083805784E-4	0.0	0.0	24.842006157848264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	7.0316234E-4	45.000004	19
GTTTACG	20	7.0316234E-4	45.000004	1
TCGATTG	20	7.0316234E-4	45.000004	1
TACGGGT	85	0.0	45.000004	4
AAACGAT	20	7.0316234E-4	45.000004	16
TATCTCG	20	7.0316234E-4	45.000004	26
TGTAGCG	30	2.16443E-6	45.000004	1
GACGCGT	20	7.0316234E-4	45.000004	39
ATCGAAT	80	0.0	45.000004	43
TAGCGCG	25	3.8894286E-5	45.0	1
CGATCGA	25	3.8894286E-5	45.0	41
GGTTACG	25	3.8894286E-5	45.0	1
CGAATAT	55	6.002665E-11	40.909092	14
GATAGCG	85	0.0	39.705883	1
TACGGCT	625	0.0	39.600002	7
CGGGTAT	80	0.0	39.375004	6
ATCACGG	40	3.4559525E-7	39.375004	2
CGGAAAG	35	6.2452145E-6	38.57143	7
CGTATGG	35	6.2452145E-6	38.57143	2
CCGATCG	35	6.2452145E-6	38.57143	40
>>END_MODULE