SRR3549786_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549786_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718610
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	2568	0.3573565633653859	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1658	0.23072320173668612	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1346	0.18730604917827473	No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	1267	0.17631260349842057	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	977	0.1359569168255382	No Hit
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	879	0.12231947788090898	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	813	0.11313508022432195	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	783	0.1089603540167824	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	741	0.10311573732622703	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	722	0.10047174406145197	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCC	20	7.0315186E-4	45.000004	42
ATTAGCG	20	7.0315186E-4	45.000004	1
AGGCGTA	20	7.0315186E-4	45.000004	11
GTTACGT	20	7.0315186E-4	45.000004	44
ATATACG	20	7.0315186E-4	45.000004	30
ACGTCGA	20	7.0315186E-4	45.000004	38
CGACTTA	20	7.0315186E-4	45.000004	40
AATACCG	20	7.0315186E-4	45.000004	31
AGTACCG	20	7.0315186E-4	45.000004	1
TACAACG	20	7.0315186E-4	45.000004	27
TACCGAT	20	7.0315186E-4	45.000004	33
CGCCAAT	20	7.0315186E-4	45.000004	14
TAATAGG	90	0.0	45.0	2
TATAGCG	45	3.8380676E-10	45.0	1
TGTACGC	55	1.8189894E-12	45.0	15
GTCGATC	25	3.889342E-5	44.999996	25
CAAGTCG	30	2.1643627E-6	44.999996	1
CCGATCA	25	3.889342E-5	44.999996	36
CCAATCG	25	3.889342E-5	44.999996	2
AATTGCG	25	3.889342E-5	44.999996	1