##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549786_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	718610
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34586632526683	33.0	31.0	34.0	30.0	34.0
2	32.444626431583195	34.0	31.0	34.0	31.0	34.0
3	32.46990439876985	34.0	31.0	34.0	30.0	34.0
4	35.96420311434575	37.0	35.0	37.0	35.0	37.0
5	36.00930685629201	37.0	35.0	37.0	35.0	37.0
6	36.05574511904928	37.0	35.0	37.0	35.0	37.0
7	36.194220787353366	37.0	37.0	37.0	35.0	37.0
8	35.870711512503306	37.0	36.0	37.0	35.0	37.0
9	37.74652175728142	39.0	38.0	39.0	35.0	39.0
10	37.63548238961328	39.0	37.0	39.0	35.0	39.0
11	37.54369407606351	39.0	37.0	39.0	35.0	39.0
12	37.33498281404378	39.0	37.0	39.0	35.0	39.0
13	37.255120301693545	39.0	37.0	39.0	34.0	39.0
14	38.46467068368099	40.0	38.0	41.0	34.0	41.0
15	38.54065348380902	40.0	38.0	41.0	35.0	41.0
16	38.59500841903119	40.0	38.0	41.0	35.0	41.0
17	38.62054661081811	40.0	38.0	41.0	35.0	41.0
18	38.603335606239824	40.0	38.0	41.0	35.0	41.0
19	38.601008892166824	40.0	37.0	41.0	35.0	41.0
20	38.5498921529063	40.0	37.0	41.0	35.0	41.0
21	38.47919873088323	40.0	37.0	41.0	35.0	41.0
22	38.457673842557156	40.0	37.0	41.0	35.0	41.0
23	38.45056289225032	40.0	37.0	41.0	35.0	41.0
24	38.392742934275894	40.0	37.0	41.0	34.0	41.0
25	38.29884638399132	40.0	37.0	41.0	34.0	41.0
26	38.27368530913848	40.0	37.0	41.0	34.0	41.0
27	38.23735823325587	40.0	37.0	41.0	34.0	41.0
28	38.17107053895715	40.0	36.0	41.0	34.0	41.0
29	38.161233492436786	40.0	36.0	41.0	34.0	41.0
30	38.061166696817466	40.0	36.0	41.0	34.0	41.0
31	38.02124518167017	40.0	36.0	41.0	34.0	41.0
32	37.9710440990245	40.0	36.0	41.0	34.0	41.0
33	37.89827027177468	40.0	36.0	41.0	34.0	41.0
34	37.82147618318699	40.0	36.0	41.0	34.0	41.0
35	37.754403640361254	40.0	36.0	41.0	33.0	41.0
36	37.661027539277214	40.0	35.0	41.0	33.0	41.0
37	37.620037294220786	40.0	35.0	41.0	33.0	41.0
38	37.53851463241536	40.0	35.0	41.0	33.0	41.0
39	37.48810620503472	40.0	35.0	41.0	33.0	41.0
40	37.38967311893795	40.0	35.0	41.0	33.0	41.0
41	37.31723605293553	39.0	35.0	41.0	33.0	41.0
42	37.23601814614325	39.0	35.0	41.0	33.0	41.0
43	37.16033036000056	39.0	35.0	41.0	33.0	41.0
44	37.090402304448865	39.0	35.0	41.0	33.0	41.0
45	37.040699405797305	39.0	35.0	41.0	33.0	41.0
46	36.897362964612235	39.0	35.0	41.0	32.0	41.0
47	36.83724969037447	39.0	35.0	41.0	32.0	41.0
48	36.77548183298312	39.0	35.0	41.0	32.0	41.0
49	36.67367139338445	39.0	35.0	41.0	32.0	41.0
50	36.611028235064914	38.0	35.0	41.0	32.0	41.0
51	35.78160059002797	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	7.0
11	13.0
12	14.0
13	17.0
14	18.0
15	23.0
16	48.0
17	84.0
18	128.0
19	199.0
20	344.0
21	516.0
22	810.0
23	1165.0
24	1810.0
25	2799.0
26	3830.0
27	4829.0
28	5569.0
29	6425.0
30	8311.0
31	10278.0
32	13601.0
33	19478.0
34	41510.0
35	67496.0
36	46958.0
37	70808.0
38	126903.0
39	284046.0
40	561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86761943195892	21.957389961175046	23.540306981533796	11.634683625332238
2	32.61351776346002	24.93285648682874	24.485186679840247	17.968439069871
3	33.037391631065525	24.045448852646082	25.4935222165013	17.42363729978709
4	30.045365358121927	25.190158778753425	24.905442451399228	19.859033411725413
5	25.71422607533989	29.63819039534657	23.996465398477618	20.65111813083592
6	26.518695815532766	34.30915239142233	23.695606796454268	15.476544996590642
7	78.53341868329136	6.900822421062886	8.989577100235175	5.576181795410584
8	78.40553290380039	6.237319269144598	9.109670057472064	6.247477769582945
9	72.18045949819792	8.866700992193262	11.691320744214526	7.261518765394302
10	43.10683124365094	23.68544829601592	18.736310376977777	14.471410083355366
11	32.90421786504501	24.461390740457272	22.576919330373915	20.057472064123793
12	29.682999123307496	22.64134927151028	27.526474722032813	20.149176883149416
13	25.805235106664252	25.020247422106568	28.393008725177776	20.781508746051404
14	20.85581887254561	28.34305116822755	28.492645523997716	22.308484435229122
15	20.782343691292912	25.955107777514925	33.12840066238989	20.13414786880227
16	22.73889870722645	25.270591837018692	30.987183590542855	21.003325865212005
17	22.205925328063902	25.454836420311434	27.600924006067267	24.738314245557397
18	23.30958377979711	25.599560262172805	29.34011494412825	21.750741013901838
19	25.08147673981715	26.74816659940719	26.471243094307066	21.6991135664686
20	27.169535631288184	26.722144139380195	26.575054619334548	19.533265609997077
21	25.140618694423956	26.726736338208486	28.00155856445081	20.131086402916743
22	23.068980392702578	25.57228538428355	27.286427965099293	24.072306257914587
23	23.112815017881744	25.648265401260765	27.834708673689484	23.404210907168004
24	23.90142079848597	24.371494969454922	29.084621700226826	22.64246253183229
25	22.561890316026773	25.92365817341813	27.04151069425697	24.47294081629813
26	21.376546388166044	27.45174712291786	27.00783456951615	24.163871919399956
27	22.788995421716926	27.868523955970552	27.660205118214332	21.68227550409819
28	20.45073127287402	27.645871891568447	28.85195029292662	23.051446542630913
29	22.89628588525069	26.104980448365595	27.33805541253253	23.660678253851188
30	22.525431040480928	27.47776958294485	27.68135706433253	22.31544231224169
31	24.113357732288723	28.69428479982188	23.961258540794034	23.231098927095363
32	25.146324153574263	27.49864321398255	24.607784472801658	22.74724815964153
33	23.51261463102378	27.901365135469863	26.604556017867832	21.981464215638525
34	22.736672186582428	26.38677446737451	27.54414772964473	23.332405616398326
35	23.956109711804736	25.634349647235638	26.513407829003217	23.896132811956416
36	23.34367737715868	29.215012315442312	26.23578853620183	21.205521771197173
37	23.932174614881507	28.829963401566914	26.410848721837993	20.827013261713585
38	22.10768010464647	28.02605029153505	25.661207052504142	24.205062551314345
39	23.912970874326824	24.029863208137932	28.69331069704012	23.36385522049512
40	24.43147186930324	23.74987823715228	29.234494371077496	22.584155522466983
41	22.641070956429772	25.323889174934944	27.147548739928475	24.88749112870681
42	22.324626709898276	26.001586395958864	27.909436272804445	23.76435062133842
43	23.621853300121067	24.541128011021275	27.505740248535364	24.331278440322286
44	22.242106288529246	25.122389056651034	26.790887964264343	25.844616690555377
45	23.561737242732498	25.012872072473247	25.972363312506086	25.45302737228817
46	23.61141648460222	26.304254046005482	26.71977846119592	23.36455100819638
47	22.78913457925718	25.13046019398561	28.80101863319464	23.279386593562574
48	21.809604653428146	23.94288974548086	29.269979543841586	24.977526057249413
49	22.636339600061227	24.261560512656377	29.738801296948274	23.363298590334118
50	21.33299007806738	23.63562989660595	29.406632248368375	25.624747776958294
51	20.785126842097938	24.392229442952367	27.026899152530582	27.795744562419117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	398.0
1	921.5
2	1445.0
3	2106.5
4	2768.0
5	1992.5
6	1217.0
7	1173.0
8	1129.0
9	1189.5
10	1250.0
11	1248.0
12	1246.0
13	1300.0
14	1354.0
15	1396.5
16	1439.0
17	1325.5
18	1212.0
19	1286.5
20	1361.0
21	1964.0
22	2567.0
23	2753.5
24	2940.0
25	3439.5
26	5060.5
27	6182.0
28	7378.5
29	8575.0
30	10570.5
31	12566.0
32	13182.5
33	13799.0
34	15543.5
35	17288.0
36	17876.5
37	18465.0
38	20130.0
39	21795.0
40	25369.0
41	28943.0
42	33415.0
43	37887.0
44	42224.5
45	46562.0
46	53506.5
47	60451.0
48	66428.5
49	72406.0
50	71493.5
51	70581.0
52	61209.5
53	51838.0
54	46521.0
55	41204.0
56	38019.0
57	34834.0
58	34697.5
59	34561.0
60	32583.5
61	30606.0
62	28257.0
63	25908.0
64	23147.0
65	20386.0
66	17549.5
67	14713.0
68	12387.0
69	10061.0
70	8310.0
71	6559.0
72	5034.5
73	3510.0
74	3010.5
75	1895.0
76	1279.0
77	929.0
78	579.0
79	365.0
80	151.0
81	133.5
82	116.0
83	69.0
84	22.0
85	11.5
86	1.0
87	1.0
88	1.0
89	3.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	718610.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.028248900381772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.56347656510349	17.410520058472844
2	12.023429221946785	6.018507584060153
3	4.7221555099466395	3.5456185034776113
4	2.3258274090594266	2.328455491730775
5	1.3512290127018578	1.690944802565961
6	0.8664363281510391	1.301123044637824
7	0.6417308975524725	1.1242980441706072
8	0.47328098091429766	0.9476315352111901
9	0.411898202983949	0.9278181671312025
>10	6.372176333615598	41.01368402284586
>50	1.1549295635825951	17.75287762927938
>100	0.08442467570048062	3.7500147221132103
>500	0.00675397405603845	1.2251184417647192
>1k	0.0022513246853461504	0.9633879525386817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	2568	0.3573565633653859	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1658	0.23072320173668612	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1346	0.18730604917827473	No Hit
CTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGCT	1267	0.17631260349842057	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	977	0.1359569168255382	No Hit
TCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	879	0.12231947788090898	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	813	0.11313508022432195	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCC	783	0.1089603540167824	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	741	0.10311573732622703	No Hit
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC	722	0.10047174406145197	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7831508050263706E-4	0.0	0.0	0.18382711067199176	0.0
2	2.7831508050263706E-4	0.0	0.0	1.0191898248006568	0.0
3	2.7831508050263706E-4	0.0	0.0	1.373206607200011	0.0
4	2.7831508050263706E-4	0.0	0.0	1.900891999833011	0.0
5	2.7831508050263706E-4	0.0	0.0	3.3646901657366306	0.0
6	2.7831508050263706E-4	0.0	0.0	4.140910925258485	0.0
7	2.7831508050263706E-4	0.0	0.0	4.942736672186583	0.0
8	2.7831508050263706E-4	0.0	0.0	6.117782942068716	0.0
9	2.7831508050263706E-4	0.0	0.0	6.608452428994865	0.0
10	2.7831508050263706E-4	0.0	0.0	7.617622910897427	0.0
11	2.7831508050263706E-4	0.0	0.0	9.092971152641907	0.0
12	2.7831508050263706E-4	0.0	0.0	10.18034817216571	0.0
13	2.7831508050263706E-4	0.0	0.0	10.652092233617678	0.0
14	2.7831508050263706E-4	0.0	0.0	10.856375502706614	0.0
15	2.7831508050263706E-4	0.0	0.0	11.253113649963124	0.0
16	2.7831508050263706E-4	0.0	0.0	12.003451106998233	0.0
17	2.7831508050263706E-4	0.0	0.0	12.911175742057583	0.0
18	2.7831508050263706E-4	0.0	0.0	13.862595844755848	0.0
19	2.7831508050263706E-4	0.0	0.0	14.43161102684349	0.0
20	2.7831508050263706E-4	0.0	0.0	14.986710454906	0.0
21	2.7831508050263706E-4	0.0	0.0	15.679993320438069	0.0
22	2.7831508050263706E-4	0.0	0.0	16.41808491393106	0.0
23	2.7831508050263706E-4	0.0	0.0	17.105801477853078	0.0
24	2.7831508050263706E-4	0.0	0.0	17.714337401372095	0.0
25	2.7831508050263706E-4	0.0	0.0	18.238126382878058	0.0
26	2.7831508050263706E-4	0.0	0.0	18.70402582763947	0.0
27	2.7831508050263706E-4	0.0	0.0	19.15433962789274	0.0
28	2.7831508050263706E-4	0.0	0.0	19.619821600033397	0.0
29	2.7831508050263706E-4	0.0	0.0	20.093235551968384	0.0
30	2.7831508050263706E-4	0.0	0.0	20.634001753385007	0.0
31	2.7831508050263706E-4	0.0	0.0	21.13566468599101	0.0
32	2.7831508050263706E-4	0.0	0.0	21.673091106441603	0.0
33	2.7831508050263706E-4	0.0	0.0	22.169326894977804	0.0
34	2.7831508050263706E-4	0.0	0.0	22.67460792363034	0.0
35	2.7831508050263706E-4	0.0	0.0	23.196170384492284	0.0
36	2.7831508050263706E-4	0.0	0.0	23.69310196072974	0.0
37	2.7831508050263706E-4	0.0	0.0	24.172499686895534	0.0
38	2.7831508050263706E-4	0.0	0.0	24.6542630912456	0.0
39	2.7831508050263706E-4	0.0	0.0	25.14841151667803	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCC	20	7.0315186E-4	45.000004	42
ATTAGCG	20	7.0315186E-4	45.000004	1
AGGCGTA	20	7.0315186E-4	45.000004	11
GTTACGT	20	7.0315186E-4	45.000004	44
ATATACG	20	7.0315186E-4	45.000004	30
ACGTCGA	20	7.0315186E-4	45.000004	38
CGACTTA	20	7.0315186E-4	45.000004	40
AATACCG	20	7.0315186E-4	45.000004	31
AGTACCG	20	7.0315186E-4	45.000004	1
TACAACG	20	7.0315186E-4	45.000004	27
TACCGAT	20	7.0315186E-4	45.000004	33
CGCCAAT	20	7.0315186E-4	45.000004	14
TAATAGG	90	0.0	45.0	2
TATAGCG	45	3.8380676E-10	45.0	1
TGTACGC	55	1.8189894E-12	45.0	15
GTCGATC	25	3.889342E-5	44.999996	25
CAAGTCG	30	2.1643627E-6	44.999996	1
CCGATCA	25	3.889342E-5	44.999996	36
CCAATCG	25	3.889342E-5	44.999996	2
AATTGCG	25	3.889342E-5	44.999996	1
>>END_MODULE