##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1016650
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43012246102395	33.0	31.0	34.0	31.0	34.0
2	32.53357891112969	34.0	31.0	34.0	31.0	34.0
3	32.55032312005115	34.0	31.0	34.0	31.0	34.0
4	36.029120149510646	37.0	35.0	37.0	35.0	37.0
5	36.07657010770669	37.0	35.0	37.0	35.0	37.0
6	36.12046918801948	37.0	36.0	37.0	35.0	37.0
7	36.28295873702847	37.0	37.0	37.0	35.0	37.0
8	36.057356022229875	37.0	37.0	37.0	35.0	37.0
9	37.93418383907933	39.0	38.0	39.0	35.0	39.0
10	37.73552943490877	39.0	38.0	39.0	35.0	39.0
11	37.63459696060591	39.0	37.0	39.0	35.0	39.0
12	37.56894211380514	39.0	37.0	39.0	35.0	39.0
13	37.57528943097428	39.0	37.0	39.0	35.0	39.0
14	38.90174199577042	40.0	38.0	41.0	35.0	41.0
15	38.96343579402941	40.0	38.0	41.0	35.0	41.0
16	39.00636994049083	40.0	38.0	41.0	35.0	41.0
17	39.00764274824177	40.0	38.0	41.0	35.0	41.0
18	38.97531205429597	40.0	38.0	41.0	35.0	41.0
19	38.934489745733536	40.0	38.0	41.0	35.0	41.0
20	38.858892440859684	40.0	38.0	41.0	35.0	41.0
21	38.817975704519746	40.0	38.0	41.0	35.0	41.0
22	38.80416564205971	40.0	38.0	41.0	35.0	41.0
23	38.770808046033544	40.0	38.0	41.0	35.0	41.0
24	38.729030639846556	40.0	38.0	41.0	35.0	41.0
25	38.66139674420892	40.0	38.0	41.0	35.0	41.0
26	38.63954064820735	40.0	38.0	41.0	35.0	41.0
27	38.60060099345891	40.0	38.0	41.0	35.0	41.0
28	38.54464073181528	40.0	38.0	41.0	35.0	41.0
29	38.52557910785423	40.0	38.0	41.0	35.0	41.0
30	38.43971868391285	40.0	38.0	41.0	35.0	41.0
31	38.37831898883588	40.0	38.0	41.0	35.0	41.0
32	38.26928539812128	40.0	38.0	41.0	34.0	41.0
33	38.178781291496584	40.0	38.0	41.0	34.0	41.0
34	38.07945015492057	40.0	37.0	41.0	34.0	41.0
35	37.994602862342006	40.0	37.0	41.0	34.0	41.0
36	37.92667191265431	40.0	37.0	41.0	34.0	41.0
37	37.87677175035656	40.0	37.0	41.0	34.0	41.0
38	37.80352333644814	40.0	37.0	41.0	34.0	41.0
39	37.74981065263365	40.0	37.0	41.0	33.0	41.0
40	37.67703437761275	40.0	37.0	41.0	33.0	41.0
41	37.60989327693897	40.0	37.0	41.0	33.0	41.0
42	37.57254905818128	40.0	36.0	41.0	33.0	41.0
43	37.50501549205725	40.0	36.0	41.0	33.0	41.0
44	37.43842718733094	40.0	36.0	41.0	33.0	41.0
45	37.38141936753062	40.0	36.0	41.0	33.0	41.0
46	37.26058722274136	40.0	35.0	41.0	33.0	41.0
47	37.232079870161805	40.0	35.0	41.0	33.0	41.0
48	37.17886981753799	40.0	35.0	41.0	33.0	41.0
49	37.085732552992674	40.0	35.0	41.0	32.0	41.0
50	37.032870702798405	39.0	35.0	41.0	32.0	41.0
51	36.309295234348106	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	9.0
11	15.0
12	21.0
13	21.0
14	23.0
15	29.0
16	61.0
17	95.0
18	150.0
19	277.0
20	447.0
21	713.0
22	1109.0
23	1793.0
24	2899.0
25	4680.0
26	7020.0
27	8386.0
28	8682.0
29	9150.0
30	10318.0
31	12376.0
32	15805.0
33	22337.0
34	42554.0
35	65582.0
36	57677.0
37	87661.0
38	173860.0
39	481755.0
40	1126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.433580878375054	20.45531893965475	25.066935523533175	12.044164658437023
2	33.568091280184916	23.558648502434465	25.712192003147592	17.16106821423302
3	31.937638321939705	23.5532385776816	27.909900162297742	16.599222938080953
4	27.40559681306251	26.434269414252693	27.456351743471203	18.703782029213595
5	25.51772979884916	30.39364579747209	25.338317021590516	18.75030738208823
6	26.135444843358087	34.650174593026115	24.16574042197413	15.048640141641666
7	82.1482319382285	5.352382825947966	8.360694437613732	4.138690798209807
8	84.25977475040575	4.468007672256922	7.71061820685585	3.561599370481483
9	78.14862538730142	7.74740569517533	9.52225446318792	4.581714454335318
10	37.43618747848325	34.977327497172084	15.833177593075295	11.753307431269365
11	25.91098214724832	26.28741454777947	29.52648404072198	18.275119264250232
12	25.014311710028032	24.465253528746373	31.09103427925048	19.429400481975115
13	23.0258200954114	24.726995524516795	32.37121920031476	19.875965179757046
14	19.910982147248315	27.939900654109085	30.88427679142281	21.26484040721979
15	19.60182953818915	27.624846308955885	33.132444794176955	19.640879358678013
16	22.508336202232822	27.402744307283726	31.415039590812967	18.67387989967049
17	22.31711995278611	26.662764963360054	29.799340972802835	21.220774111051
18	22.155805832882507	26.956179609501795	30.673191363792853	20.214823193822852
19	22.63719077361924	27.194904834505486	30.11911670683126	20.04878768504402
20	24.367678158658336	27.692519549500812	29.402154133674323	18.53764815816653
21	24.030590665420746	26.942113805144345	29.728618501942655	19.298677027492253
22	22.94142526926671	25.08021442974475	29.580189839177688	22.39817046181085
23	21.145526975852064	27.10893621206905	30.616436335021884	21.129100477057
24	21.04067279791472	26.587911277234056	31.165002704962376	21.20641321988885
25	21.355038607190284	28.585452220528207	28.733290709683768	21.326218462597748
26	21.18172429056214	29.333989081788225	28.5848620469188	20.899424580730834
27	21.581763635469432	28.25259430482467	29.551664781389857	20.613977278316035
28	19.524615157625536	28.290758864899423	30.98824570894605	21.19638026852899
29	20.97899965573206	26.87680125903703	30.403678748831947	21.74052033639896
30	21.987606354202526	26.736733389071954	30.091280184921064	21.18438007180446
31	22.20479024246299	27.365858456696014	28.151084444007278	22.278266856833717
32	21.747700781980033	28.21639699011459	28.521713470712633	21.51418875719274
33	22.17282250528697	27.060935425170907	29.012541189199826	21.7537008803423
34	20.50164756799292	27.175330743126935	29.966950277873405	22.356071411006738
35	21.447597501598388	26.408105050902474	29.379039000639356	22.765258446859786
36	22.148822111837898	28.093247430285746	28.13819898686864	21.61973147100772
37	21.752127084050557	27.157133723503662	29.511237889145725	21.579501303300056
38	20.966507647666354	26.77224216790439	29.61087886686667	22.650371317562584
39	21.78065214183839	25.639305562386266	30.24679093099887	22.333251364776473
40	22.260561648551615	25.443761373137264	31.060443613829737	21.235233364481385
41	20.647420449515565	26.573845472876606	29.6214036295677	23.15733044804013
42	22.45433531697241	26.966409285398118	28.000098362268233	22.579157035361234
43	22.535090739192444	25.759405891899867	29.043820390498205	22.66168297840948
44	21.688093247430285	26.380858702601685	28.820046230266072	23.11100181970196
45	21.960851817242904	25.309005065656816	29.04824669256873	23.68189642453155
46	21.803078738995723	26.588403088575223	28.756996016328134	22.85152215610092
47	21.14601878719323	25.67530615255988	30.86126002065608	22.317415039590813
48	21.107460778045542	25.37913736290759	30.737323562681357	22.776078296365515
49	21.821472483155464	24.95549107362416	30.400727880784935	22.82230856243545
50	20.536074361874785	25.040574435646484	30.71204445974524	23.711306742733488
51	20.64850243446614	24.358530467712587	29.602026262725616	25.39094083509566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	295.0
1	699.0
2	1103.0
3	5335.0
4	9567.0
5	6385.0
6	3203.0
7	3095.5
8	2988.0
9	3018.0
10	3048.0
11	3049.0
12	3050.0
13	2825.5
14	2601.0
15	2611.5
16	2622.0
17	2643.5
18	2665.0
19	2860.5
20	3056.0
21	3495.5
22	3935.0
23	4447.0
24	4959.0
25	6354.5
26	9157.5
27	10565.0
28	12976.5
29	15388.0
30	18154.5
31	20921.0
32	23260.5
33	25600.0
34	29585.0
35	33570.0
36	35921.5
37	38273.0
38	42180.5
39	46088.0
40	49693.0
41	53298.0
42	58045.0
43	62792.0
44	67646.5
45	72501.0
46	79188.5
47	85876.0
48	91298.5
49	96721.0
50	93996.0
51	91271.0
52	81145.5
53	71020.0
54	63127.5
55	55235.0
56	50380.0
57	45525.0
58	41169.0
59	36813.0
60	34634.5
61	32456.0
62	28773.0
63	25090.0
64	21706.0
65	18322.0
66	14504.5
67	10687.0
68	8951.5
69	7216.0
70	5609.0
71	4002.0
72	3588.5
73	3175.0
74	2435.5
75	1316.5
76	937.0
77	684.0
78	431.0
79	353.5
80	276.0
81	153.0
82	30.0
83	31.0
84	32.0
85	16.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1016650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.07737529199006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65837537906292	19.40320722953187
2	10.275576410736043	5.5647127763004
3	3.8028439927189157	3.089131019032199
4	1.8687736697386117	2.0240594396520746
5	1.2361555957368993	1.673592449253078
6	0.898770853783663	1.4601813425641537
7	0.6635722416529723	1.2577456234409252
8	0.5763373552467207	1.248456229024676
9	0.4892996694921295	1.192405570297658
>10	8.014720966447365	51.742346485370014
>50	0.46652428661016965	7.598025494348559
>100	0.04536164052000533	2.318542239420465
>500	0.0022127629521953	0.4436765944415936
>1k	0.00110638147609765	0.3939464697438862
>5k	3.6879382536588335E-4	0.5899710375784233
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5908	0.5811242807259136	No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT	1504	0.1479368514237938	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	1384	0.1361333792357252	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1057	0.10396891752323809	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15354350071312645	0.0
2	0.0	0.0	0.0	0.49672945458122264	0.0
3	0.0	0.0	0.0	0.6925687306349284	0.0
4	0.0	0.0	0.0	0.9539172773324153	0.0
5	0.0	0.0	0.0	1.7088476860276398	0.0
6	0.0	0.0	0.0	2.2983327595534355	0.0
7	0.0	0.0	0.0	2.8177838980966903	0.0
8	0.0	0.0	0.0	3.633600550828702	0.0
9	0.0	0.0	0.0	4.0379678355382875	0.0
10	0.0	0.0	0.0	4.886735848128658	0.0
11	0.0	0.0	0.0	6.067574878276693	0.0
12	0.0	0.0	0.0	7.033393990065411	0.0
13	0.0	0.0	0.0	7.454679584911228	0.0
14	0.0	0.0	0.0	7.649043426941425	0.0
15	0.0	0.0	0.0	7.953868096198298	0.0
16	0.0	0.0	0.0	8.503024639748192	0.0
17	0.0	0.0	0.0	9.219593763832194	0.0
18	0.0	0.0	0.0	9.945507303398417	0.0
19	0.0	0.0	0.0	10.429252938572764	0.0
20	0.0	0.0	0.0	10.90414596960606	0.0
21	0.0	0.0	0.0	11.479073427433237	0.0
22	0.0	0.0	0.0	12.1201003295136	0.0
23	0.0	0.0	0.0	12.723552845128609	0.0
24	0.0	0.0	0.0	13.20641321988885	0.0
25	0.0	0.0	0.0	13.636354694339252	0.0
26	0.0	0.0	0.0	14.02970540500664	0.0
27	0.0	0.0	0.0	14.423547927015196	0.0
28	0.0	0.0	0.0	14.849063099395073	0.0
29	0.0	0.0	0.0	15.299660650174593	0.0
30	0.0	0.0	0.0	15.829538189150641	0.0
31	0.0	0.0	0.0	16.30354595976983	0.0
32	9.836226823390547E-5	0.0	0.0	16.76614370727389	0.0
33	9.836226823390547E-5	0.0	0.0	17.202970540500665	0.0
34	9.836226823390547E-5	0.0	0.0	17.621305267299466	0.0
35	9.836226823390547E-5	0.0	0.0	18.0684601386908	0.0
36	9.836226823390547E-5	0.0	0.0	18.522008557517335	0.0
37	9.836226823390547E-5	0.0	0.0	18.960310824767618	0.0
38	9.836226823390547E-5	0.0	0.0	19.395170412629714	0.0
39	1.9672453646781094E-4	0.0	0.0	19.840358038656372	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGGGC	35	1.211738E-7	45.000004	4
GCGCGAT	20	7.0329016E-4	45.0	40
GTAAGCG	50	2.1827873E-11	45.0	1
TATCGTC	20	7.0329016E-4	45.0	32
TCGCGAA	20	7.0329016E-4	45.0	42
AATCGCG	20	7.0329016E-4	45.0	1
GTCGCGC	30	2.165254E-6	44.999996	38
TCGTGCG	30	2.165254E-6	44.999996	1
CGTTTTT	3030	0.0	42.549507	1
GCCGATA	60	3.6379788E-12	41.249996	9
ACGGGTA	110	0.0	40.909092	5
AGTAACG	55	6.184564E-11	40.909092	1
TAATGCG	50	1.0804797E-9	40.5	1
CGACAGG	160	0.0	39.375	2
ACACGAA	35	6.2475756E-6	38.57143	23
TACGCGG	105	0.0	38.57143	2
GCGTACG	35	6.2475756E-6	38.57143	1
AACACGT	165	0.0	38.181816	41
CGGACGG	95	0.0	37.894737	2
TCGGCGT	180	0.0	37.5	4
>>END_MODULE