FastQCFastQC Report
Sat 18 Jun 2016
SRR3549781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences738810
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17880.2420108011532058No Hit
GCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC13270.17961316170598665No Hit
CCTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGC11180.15132442711928643No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA10570.1430679064982878No Hit
CTGTCTCTTATACACATCTGACGCTCATCTGGTCGTATGCCGTCTTCTGCT10330.13981943936871455Illumina Single End Adapter 1 (95% over 21bp)
ATCCGCTAGTTCTGGCCCCTGGCCTCTGGCATAATATCTTCACAATGGTGC8530.11545593589691532No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG8140.1101771768113588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACGG302.1644446E-645.0000042
CGGGTAC253.8894483E-545.06
CGATTCT253.8894483E-545.010
TAACGAC207.0316484E-445.034
ACGATGT207.0316484E-445.016
ACGATAC207.0316484E-445.040
GCCGACC253.8894483E-545.014
GTATACG207.0316484E-445.025
TTACGAG253.8894483E-545.01
GATTACG253.8894483E-545.01
ACGGTTG406.8084773E-945.01
GTTACGG700.041.7857132
CTACGAA1300.041.53846411
CGCATGG1000.040.52
ACGGGTA501.0804797E-940.55
TTAATCG451.9266736E-840.020
TTACGGG1750.039.8571433
CGAATAT1300.039.80769314
GCGATTC403.455989E-739.3759
ATCGGGT403.455989E-739.3753