FastQCFastQC Report
Sat 18 Jun 2016
SRR3549779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030045
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255852.4838720638418708No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC125481.2181992048891068No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG59840.5809454926726503No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT49530.4808527782766772No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC45440.44114577518457926No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC38740.37610007329776857No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT36610.35542136508599137No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT29770.2890164992791577No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC25320.24581450324985804TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG18280.17746797470013445No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT17640.17125465392288686No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT15600.15144969394541014No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT13890.13484847749370174No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC13070.12688766024785325No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC12570.12203350339062857No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC12480.12115975515632812No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC12440.12077142260775015TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT11400.11067477634472281No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT10400.10096646263027342TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGT10320.10018979753311748No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCG207.0329435E-445.0000041
ATAACGG302.1652813E-645.0000042
CGTCCCA207.0329435E-445.00000438
TAATGCG207.0329435E-445.0000041
CGTTTTT127300.044.346031
CGATGAA8850.040.932219
TACGACG556.184564E-1140.909091
CGTTTCT6250.040.321
TAGTGCG403.4577533E-739.3750041
GTTTTTT145150.039.078542
CGTTCTG4550.039.0659331
CCGATGA8900.038.67977518
TACGGCT6200.038.4677437
CATACGA659.094947E-1238.0769218
AGTAGCG659.094947E-1238.076921
TCCGATT301.13983944E-437.50000430
ACGTCCG301.13983944E-437.5000041
AATGCGG1100.036.818182
CGCCGGT1850.036.4864928
TTTAGCG751.8189894E-1236.0000041
Produced by FastQC (version 0.11.5)