##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030045
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.299450024028076	33.0	31.0	34.0	30.0	34.0
2	32.45852365673344	34.0	31.0	34.0	30.0	34.0
3	32.39514001815455	34.0	31.0	34.0	30.0	34.0
4	35.97986592818761	37.0	35.0	37.0	35.0	37.0
5	36.009619968059646	37.0	35.0	37.0	35.0	37.0
6	36.03481692547413	37.0	35.0	37.0	35.0	37.0
7	36.188219932138885	37.0	37.0	37.0	35.0	37.0
8	35.876114150352656	37.0	36.0	37.0	35.0	37.0
9	37.77716895863773	39.0	38.0	39.0	35.0	39.0
10	37.65700139314302	39.0	37.0	39.0	35.0	39.0
11	37.5768165468499	39.0	37.0	39.0	35.0	39.0
12	37.52383827891015	39.0	37.0	39.0	35.0	39.0
13	37.512147527535205	39.0	37.0	39.0	35.0	39.0
14	38.85538204641545	40.0	38.0	41.0	35.0	41.0
15	38.8670145479081	40.0	38.0	41.0	35.0	41.0
16	38.94159769718799	40.0	38.0	41.0	35.0	41.0
17	38.903010062667164	40.0	38.0	41.0	35.0	41.0
18	38.6924454756831	40.0	38.0	41.0	35.0	41.0
19	38.47265896150168	40.0	37.0	41.0	35.0	41.0
20	38.17242450572548	40.0	35.0	41.0	34.0	41.0
21	38.16324044095161	40.0	35.0	41.0	34.0	41.0
22	38.14386555927168	40.0	35.0	41.0	35.0	41.0
23	38.078280075142345	40.0	35.0	41.0	34.0	41.0
24	38.01822444650477	40.0	35.0	41.0	34.0	41.0
25	37.97937371668228	40.0	35.0	41.0	34.0	41.0
26	37.92580421243732	40.0	35.0	41.0	34.0	41.0
27	37.85893626006631	40.0	35.0	41.0	34.0	41.0
28	37.757255265546654	40.0	35.0	41.0	34.0	41.0
29	37.7104136227058	40.0	35.0	41.0	34.0	41.0
30	37.53371940060871	40.0	35.0	41.0	33.0	41.0
31	37.31888315559029	40.0	35.0	41.0	33.0	41.0
32	36.88664961239557	40.0	35.0	41.0	31.0	41.0
33	36.49363377328175	40.0	35.0	41.0	30.0	41.0
34	36.17011781038693	40.0	35.0	41.0	27.0	41.0
35	35.90668368857671	40.0	35.0	41.0	24.0	41.0
36	35.760200767927614	40.0	35.0	41.0	23.0	41.0
37	35.67064254474319	40.0	35.0	41.0	23.0	41.0
38	35.5610580120286	40.0	35.0	41.0	22.0	41.0
39	35.46821740797732	40.0	35.0	41.0	22.0	41.0
40	35.36633156803829	39.0	35.0	41.0	21.0	41.0
41	35.28560985199676	39.0	35.0	41.0	21.0	41.0
42	35.19640598226291	39.0	35.0	41.0	21.0	41.0
43	35.124939201685365	39.0	35.0	41.0	20.0	41.0
44	35.09570358576567	39.0	35.0	41.0	20.0	41.0
45	35.06468261095389	39.0	35.0	41.0	20.0	41.0
46	34.9539932721386	39.0	35.0	41.0	20.0	41.0
47	34.876369479003344	39.0	35.0	41.0	20.0	41.0
48	34.84457863491401	39.0	35.0	41.0	20.0	41.0
49	34.77181967778107	39.0	35.0	41.0	20.0	41.0
50	34.713501837298374	39.0	35.0	41.0	20.0	41.0
51	34.06179438762384	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	9.0
11	16.0
12	14.0
13	18.0
14	31.0
15	41.0
16	65.0
17	161.0
18	294.0
19	576.0
20	1087.0
21	2076.0
22	3442.0
23	5017.0
24	7958.0
25	14119.0
26	21449.0
27	24100.0
28	21936.0
29	18886.0
30	16706.0
31	17553.0
32	20272.0
33	25951.0
34	44967.0
35	64908.0
36	65461.0
37	85711.0
38	159270.0
39	407189.0
40	754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.40890446533889	18.03532855360688	20.919765641306935	22.636001339747292
2	40.22882495424957	24.159235761544398	20.701619832143255	14.910319452062772
3	26.268561082282815	24.65338892960987	34.64576790334403	14.432282084763287
4	23.479071302710075	21.85710333043702	38.41987485983622	16.243950507016685
5	24.59717779320321	24.963666635923673	33.16427923051906	17.274876340354062
6	22.619885538981308	30.780014465387435	32.69371726477969	13.906382730851567
7	69.61977389337359	4.10156837808057	21.861374988471376	4.417282740074463
8	69.93053701537312	6.181477508264202	19.3626492046464	4.525336271716284
9	64.31253003509555	6.075656888776704	20.723075205452187	8.888737870675554
10	34.1093835706207	26.0634244134965	26.773005062885602	13.054186952997199
11	23.581591095534662	21.256255794649746	37.03692557121291	18.12522753860268
12	21.080535316418214	19.527302205243462	38.85587522875214	20.53628724958618
13	20.953647656170364	20.29921022867933	41.318777334970804	17.428364780179507
14	17.18924901339262	25.596842856380057	38.24376604905611	18.97014208117121
15	16.727036197447685	24.449514341606434	40.82268250416244	18.000766956783444
16	19.43866530103054	25.234334422282522	36.62558431913169	18.701415957555252
17	19.5195355542719	25.726351761330818	36.09900538325995	18.65510730113733
18	19.62642408826799	24.64775810765549	36.07376376760239	19.652054036474134
19	19.002664932114616	26.313025159094995	34.28976403943517	20.39454586935522
20	21.03374124431457	25.681111019421483	35.83222092238689	17.452926813877063
21	20.181836715871636	26.49039605065798	36.42083598289395	16.90693125057643
22	19.8280657641171	21.72662359411482	37.43923809153969	21.00607255022839
23	19.32857302350868	24.28806508453514	37.18420069026111	19.199161201695073
24	19.88291773660374	23.807212306258467	36.53335533884442	19.776514618293376
25	19.376823342669493	27.46705241033159	33.83997786504473	19.316146381954187
26	18.957230023930993	25.011722788810197	35.58854224815421	20.4425049391046
27	21.036168322743183	24.04477474285104	34.7951788514094	20.123878082996374
28	18.28823012586829	24.433689790251883	36.144925707129296	21.13315437675053
29	19.091884335150404	22.3599939808455	35.64698629671519	22.901135387288903
30	18.60782781334796	23.563727798300075	37.276429670548374	20.55201471780359
31	19.588949997330214	22.08592828468659	35.77270895931731	22.55241275866588
32	19.947186773393398	23.01918848205661	35.15186229727828	21.881762447271722
33	19.25381900790742	22.809003490138778	35.43660713852307	22.500570363430725
34	18.317355067011633	23.267430063735077	34.48946405254139	23.92575081671189
35	19.22653864636982	23.08685542864632	35.21574300151935	22.47086292346451
36	20.345615968234398	24.9076496657913	33.838327451713276	20.908406914261025
37	20.09533564067589	24.184186127790532	34.308209835492626	21.41226839604095
38	19.794280832390818	24.308646709609775	33.66435446995034	22.232717988049068
39	21.51488527200268	24.305054633535427	31.849191054759746	22.33086903970215
40	21.004519220034076	23.665665092301793	34.61926420690358	20.71055148076055
41	20.162128839031304	25.40189991699392	32.238203185297735	22.19776805867705
42	20.338237649811415	25.28540015242052	33.783960894912354	20.592401302855702
43	22.15262439990486	24.276900523763526	32.1982049327942	21.37227014353742
44	21.042575809794716	24.34379080525608	31.724827556077646	22.888805828871554
45	20.37347882859487	23.259469246489232	31.556970811954816	24.810081112961086
46	22.01680509103971	24.54368498463659	30.79943109281633	22.640078831507363
47	19.810008300608224	24.28000718415215	34.26966783004626	21.640316685193365
48	20.033882014863426	23.334320345227635	33.32019474877311	23.311602891135824
49	19.591862491444548	22.59056643156367	35.12176652476348	22.695804552228303
50	19.065574804984248	22.818226388167506	35.15166813100399	22.96453067584426
51	18.939755059244984	22.529306972025495	33.419510798071926	25.111427170657592
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	400.0
1	764.5
2	1129.0
3	20342.0
4	39555.0
5	25856.5
6	12158.0
7	11765.0
8	11372.0
9	11300.0
10	11228.0
11	11108.0
12	10988.0
13	10595.5
14	10203.0
15	9630.0
16	9057.0
17	8415.5
18	7774.0
19	7394.5
20	7015.0
21	6685.0
22	6355.0
23	6391.5
24	6428.0
25	6967.0
26	8451.5
27	9397.0
28	11177.0
29	12957.0
30	15205.0
31	17453.0
32	19134.0
33	20815.0
34	23363.0
35	25911.0
36	27980.0
37	30049.0
38	32695.0
39	35341.0
40	39234.0
41	43127.0
42	46461.5
43	49796.0
44	54910.0
45	60024.0
46	86974.5
47	113925.0
48	104395.5
49	94866.0
50	90406.0
51	85946.0
52	75067.5
53	64189.0
54	57682.0
55	51175.0
56	46998.0
57	42821.0
58	38773.5
59	34726.0
60	31170.5
61	27615.0
62	25013.0
63	22411.0
64	19666.5
65	16922.0
66	13824.5
67	10727.0
68	8943.5
69	7160.0
70	6056.0
71	4952.0
72	3952.0
73	2952.0
74	2303.5
75	1436.0
76	1217.0
77	780.0
78	343.0
79	286.0
80	229.0
81	161.5
82	94.0
83	68.5
84	43.0
85	33.0
86	23.0
87	18.5
88	14.0
89	7.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1030045.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.892898262295766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36010629563921	25.20279517451599
2	8.21635041156755	5.569518571732619
3	3.365935743949228	3.4224395318128797
4	2.0791382941118393	2.81872090702303
5	1.4769262787439812	2.502865605319042
6	1.1908609238726215	2.4217036882414984
7	0.9874502330312005	2.342728520104471
8	0.8515049504221294	2.3087976523598743
9	0.7472257098452245	2.279308046648034
>10	6.620215004105294	39.486902913520474
>50	0.0681761794264064	1.5986803233771656
>100	0.029176860031294084	1.8075116637114599
>500	0.0011555192090088916	0.2616148185403265
>1k	0.004910956638287789	3.656928694790866
>5k	2.888798022522229E-4	0.5858918690663599
>10k+	5.777596045044458E-4	3.73359201923588
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25585	2.4838720638418708	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	12548	1.2181992048891068	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCG	5984	0.5809454926726503	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	4953	0.4808527782766772	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGC	4544	0.44114577518457926	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTC	3874	0.37610007329776857	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	3661	0.35542136508599137	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	2977	0.2890164992791577	No Hit
GCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	2532	0.24581450324985804	TruSeq Adapter, Index 16 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTG	1828	0.17746797470013445	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCT	1764	0.17125465392288686	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTT	1560	0.15144969394541014	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCT	1389	0.13484847749370174	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCC	1307	0.12688766024785325	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC	1257	0.12203350339062857	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTC	1248	0.12115975515632812	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGC	1244	0.12077142260775015	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCTCTGT	1140	0.11067477634472281	No Hit
CTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGTCTTCTGCT	1040	0.10096646263027342	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTCTCTGTCGTATGCCGT	1032	0.10018979753311748	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9416627428898737E-4	0.0	0.0	0.10407312301889723	0.0
2	1.9416627428898737E-4	0.0	0.0	0.538228912329073	0.0
3	1.9416627428898737E-4	0.0	0.0	0.9329689479585843	0.0
4	1.9416627428898737E-4	0.0	0.0	1.7420598129207947	0.0
5	1.9416627428898737E-4	0.0	0.0	4.119820007863734	0.0
6	1.9416627428898737E-4	0.0	0.0	5.884208942327763	0.0
7	1.9416627428898737E-4	0.0	0.0	7.154347625589173	0.0
8	1.9416627428898737E-4	0.0	0.0	8.526229436578014	0.0
9	1.9416627428898737E-4	0.0	0.0	9.334543636443067	0.0
10	1.9416627428898737E-4	0.0	0.0	10.894184234669359	0.0
11	1.9416627428898737E-4	0.0	0.0	11.7864753481644	0.0
12	1.9416627428898737E-4	0.0	0.0	12.95807464722415	0.0
13	1.9416627428898737E-4	0.0	0.0	13.425238703163455	0.0
14	1.9416627428898737E-4	0.0	0.0	13.700469396968094	0.0
15	1.9416627428898737E-4	0.0	0.0	13.997155464081667	0.0
16	1.9416627428898737E-4	0.0	0.0	14.346946007213278	0.0
17	1.9416627428898737E-4	0.0	0.0	14.755277682043017	0.0
18	1.9416627428898737E-4	0.0	0.0	15.205258022707746	0.0
19	1.9416627428898737E-4	0.0	0.0	15.639025479469344	0.0
20	1.9416627428898737E-4	0.0	0.0	15.970661475954934	0.0
21	2.912494114334811E-4	0.0	0.0	16.32598575790378	0.0
22	3.8833254857797473E-4	0.0	0.0	16.74528782723085	0.0
23	3.8833254857797473E-4	0.0	0.0	17.127018722482998	0.0
24	3.8833254857797473E-4	0.0	0.0	17.450402652311308	0.0
25	3.8833254857797473E-4	0.0	0.0	17.755243702945016	0.0
26	3.8833254857797473E-4	0.0	0.0	18.038823546544084	0.0
27	3.8833254857797473E-4	0.0	0.0	18.37162454067541	0.0
28	3.8833254857797473E-4	0.0	0.0	18.677921838366284	0.0
29	3.8833254857797473E-4	0.0	0.0	19.007422005834698	0.0
30	3.8833254857797473E-4	0.0	0.0	19.391094563829736	0.0
31	3.8833254857797473E-4	0.0	0.0	19.735933866966977	0.0
32	3.8833254857797473E-4	0.0	0.0	20.1084418641904	0.0
33	3.8833254857797473E-4	0.0	0.0	20.454931580659096	0.0
34	3.8833254857797473E-4	0.0	0.0	20.780257173230297	0.0
35	3.8833254857797473E-4	0.0	0.0	21.1204364857846	0.0
36	3.8833254857797473E-4	0.0	0.0	21.46042163206462	0.0
37	3.8833254857797473E-4	0.0	0.0	21.791475129727342	0.0
38	3.8833254857797473E-4	0.0	0.0	22.152818566179146	0.0
39	3.8833254857797473E-4	0.0	0.0	22.53202529986554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACGCG	20	7.0329435E-4	45.000004	1
ATAACGG	30	2.1652813E-6	45.000004	2
CGTCCCA	20	7.0329435E-4	45.000004	38
TAATGCG	20	7.0329435E-4	45.000004	1
CGTTTTT	12730	0.0	44.34603	1
CGATGAA	885	0.0	40.9322	19
TACGACG	55	6.184564E-11	40.90909	1
CGTTTCT	625	0.0	40.32	1
TAGTGCG	40	3.4577533E-7	39.375004	1
GTTTTTT	14515	0.0	39.07854	2
CGTTCTG	455	0.0	39.065933	1
CCGATGA	890	0.0	38.679775	18
TACGGCT	620	0.0	38.467743	7
CATACGA	65	9.094947E-12	38.07692	18
AGTAGCG	65	9.094947E-12	38.07692	1
TCCGATT	30	1.13983944E-4	37.500004	30
ACGTCCG	30	1.13983944E-4	37.500004	1
AATGCGG	110	0.0	36.81818	2
CGCCGGT	185	0.0	36.48649	28
TTTAGCG	75	1.8189894E-12	36.000004	1
>>END_MODULE