SRR3549776_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772328
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	4501	0.5827834805937374	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	4098	0.5306035777545292	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	3864	0.5003055696543438	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3723	0.48204907759397564	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	2224	0.28796055561885625	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1763	0.2282708900881491	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1615	0.20910804735811728	No Hit
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1279	0.1656032152142613	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1029	0.13323354844055893	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	922	0.11937933106141432	No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	838	0.10850312302545034	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	798	0.10332397634165795	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGC	20	7.0318463E-4	45.000004	20
AACGTCA	20	7.0318463E-4	45.000004	35
CGAACCT	20	7.0318463E-4	45.000004	44
CGAACCG	20	7.0318463E-4	45.000004	14
ACCGAAC	20	7.0318463E-4	45.000004	12
CACGAGA	20	7.0318463E-4	45.000004	37
CGCTATA	20	7.0318463E-4	45.000004	20
ATAAGCG	20	7.0318463E-4	45.000004	33
CGTAAGT	20	7.0318463E-4	45.000004	22
TTTGCGC	20	7.0318463E-4	45.000004	13
ACGAGTC	20	7.0318463E-4	45.000004	24
TACGTAG	20	7.0318463E-4	45.000004	1
ACGAACC	20	7.0318463E-4	45.000004	43
GTAATCG	25	3.889613E-5	45.0	1
GCCGATA	25	3.889613E-5	45.0	9
TCGTACA	25	3.889613E-5	45.0	24
CGTCCGA	25	3.889613E-5	45.0	28
TAATGCG	35	1.2112287E-7	45.0	1
CGGACGG	50	2.1827873E-11	45.0	2
ATAGGCG	30	2.1645737E-6	44.999996	1