##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549776_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772328
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28594975191887	33.0	31.0	34.0	30.0	34.0
2	32.39676795351198	34.0	31.0	34.0	30.0	34.0
3	32.42695978910515	34.0	31.0	34.0	30.0	34.0
4	35.937600345967	37.0	35.0	37.0	35.0	37.0
5	35.98504650873722	37.0	35.0	37.0	35.0	37.0
6	36.01443039744772	37.0	35.0	37.0	35.0	37.0
7	36.19439538641613	37.0	36.0	37.0	35.0	37.0
8	35.87070907697248	37.0	35.0	37.0	35.0	37.0
9	37.79085828818844	39.0	38.0	39.0	35.0	39.0
10	37.58299064646109	39.0	37.0	39.0	35.0	39.0
11	37.500905055882995	39.0	37.0	39.0	35.0	39.0
12	37.354219968717956	39.0	37.0	39.0	35.0	39.0
13	37.25603759024663	39.0	37.0	39.0	34.0	39.0
14	38.49499823909013	40.0	38.0	41.0	34.0	41.0
15	38.552938647828384	40.0	38.0	41.0	35.0	41.0
16	38.66108052537264	40.0	38.0	41.0	35.0	41.0
17	38.66412586362271	40.0	38.0	41.0	35.0	41.0
18	38.66713235827265	40.0	38.0	41.0	35.0	41.0
19	38.67062180835086	40.0	38.0	41.0	35.0	41.0
20	38.57052961953988	40.0	38.0	41.0	35.0	41.0
21	38.56624387565905	40.0	38.0	41.0	35.0	41.0
22	38.543080142115784	40.0	38.0	41.0	35.0	41.0
23	38.50135175728447	40.0	37.0	41.0	35.0	41.0
24	38.44852316632311	40.0	37.0	41.0	34.0	41.0
25	38.40225008027677	40.0	37.0	41.0	34.0	41.0
26	38.39010498130328	40.0	37.0	41.0	34.0	41.0
27	38.345210843061494	40.0	37.0	41.0	34.0	41.0
28	38.307405661843156	40.0	37.0	41.0	34.0	41.0
29	38.307404367056485	40.0	37.0	41.0	34.0	41.0
30	38.25166898001885	40.0	37.0	41.0	34.0	41.0
31	38.19188220548782	40.0	37.0	41.0	34.0	41.0
32	38.13353782330823	40.0	37.0	41.0	34.0	41.0
33	38.09001097979097	40.0	37.0	41.0	34.0	41.0
34	38.02274033830186	40.0	37.0	41.0	34.0	41.0
35	37.98273531452958	40.0	37.0	41.0	34.0	41.0
36	37.942146341968694	40.0	37.0	41.0	34.0	41.0
37	37.875514030308366	40.0	36.0	41.0	34.0	41.0
38	37.829910348970905	40.0	36.0	41.0	34.0	41.0
39	37.788733543261415	40.0	36.0	41.0	33.0	41.0
40	37.720493106555764	40.0	36.0	41.0	33.0	41.0
41	37.631218860380564	40.0	36.0	41.0	33.0	41.0
42	37.63649511606468	40.0	36.0	41.0	33.0	41.0
43	37.60473011466631	40.0	36.0	41.0	33.0	41.0
44	37.5637410530241	40.0	35.0	41.0	33.0	41.0
45	37.517175345190125	40.0	35.0	41.0	33.0	41.0
46	37.40232388311702	40.0	35.0	41.0	33.0	41.0
47	37.32792155664433	39.0	35.0	41.0	33.0	41.0
48	37.31344325208978	39.0	35.0	41.0	33.0	41.0
49	37.238494525641954	39.0	35.0	41.0	33.0	41.0
50	37.18425202763593	39.0	35.0	41.0	33.0	41.0
51	36.454745911063696	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	0.0
10	5.0
11	11.0
12	9.0
13	11.0
14	13.0
15	30.0
16	35.0
17	59.0
18	129.0
19	198.0
20	345.0
21	595.0
22	1017.0
23	1473.0
24	2072.0
25	2931.0
26	3918.0
27	4799.0
28	5563.0
29	6688.0
30	8179.0
31	10696.0
32	14016.0
33	19780.0
34	39322.0
35	62862.0
36	45531.0
37	68321.0
38	131968.0
39	341055.0
40	691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90528894459349	21.87205436032359	25.470784433556727	11.75187226152619
2	30.558389699713075	26.287794822924976	25.063444546876458	18.090370930485495
3	30.9345252276235	25.436731544110792	26.202468381309497	17.426274846956215
4	28.53127168767674	24.039915683491987	28.876073378150213	18.552739250681057
5	26.608384002651725	28.592774054546773	24.130291793124165	20.66855014967734
6	24.364777659232864	35.28656218601423	24.626195088097287	15.722465066655616
7	78.60015433857119	5.463611315399675	10.489196300017609	5.447038046011539
8	79.35876984907966	6.380061321096736	8.567991837664826	5.693176992158771
9	74.68264778695061	6.228700759262904	10.419536777120602	8.669114676665872
10	41.559674128090656	23.45441315088926	18.54289887198185	16.44301384903823
11	29.12739147098124	27.304590795620516	23.737971431826892	19.830046301571354
12	28.45384344475404	21.89251198972457	28.550693487740958	21.102951077780425
13	24.131586579795115	24.29485917900167	31.281528055437587	20.292026185765632
14	19.211915144860733	28.03899379538227	29.826835230627402	22.922255829129593
15	17.23153893164562	26.77631783387369	35.559632694917184	20.432510539563502
16	19.178380170083177	25.68196413958836	32.2341026092541	22.905553081074363
17	19.83936876560218	26.44860732745673	29.296231652872873	24.41579225406822
18	20.171093110699083	26.223961840047235	31.065428160056346	22.539516889197337
19	21.598983851420638	27.131477817714757	28.299375394909937	22.970162935954672
20	23.430330118809625	27.300576956940574	29.208445116582592	20.06064780766721
21	23.107539801742266	27.43911913073202	28.865844563449727	20.58749650407599
22	20.68525289773257	25.015796397385564	29.083757160170293	25.21519354471157
23	20.63488569623269	26.466863819517094	30.070255124765644	22.82799535948457
24	22.098512549072417	25.01022881470049	29.055789768077812	23.835468868149285
25	19.74976952797257	28.58513481318818	27.979692565852847	23.685403092986398
26	18.91061828653111	28.047150951409243	28.394930651225902	24.64730011083374
27	20.367123812680624	27.920391336323426	29.437233921339118	22.275250929656828
28	17.46757854175946	27.81331247863602	30.719202204244827	23.99990677535969
29	19.240270972954495	25.361633917195803	29.60167182854953	25.796423281300175
30	19.63026071824406	27.289182834236232	29.414963590598813	23.665592856920895
31	19.66198299168229	29.946732476357198	25.635481298101325	24.75580323385919
32	20.75853782330823	29.020312613293836	25.743466506458397	24.47768305693954
33	19.950202504635335	27.030484457380805	26.079334168902328	26.939978869081532
34	18.850928621000403	28.036404222040378	26.797034420608863	26.31563273635036
35	18.696719528490487	25.213380843372246	27.308993070301735	28.78090655783553
36	19.51903854320962	27.285686910224673	26.529272537056798	26.666002009508915
37	18.63456976828498	29.11988170828974	29.42402709729545	22.82152142612983
38	18.076775670440536	29.043489294703807	26.138376441097567	26.74135859375809
39	20.08201178773785	26.977009767870648	27.326213733025345	25.614764711366156
40	20.09457121844605	24.83763375146311	29.078707492153594	25.98908753793725
41	17.663609243741003	26.157021369159217	27.719828880993568	28.45954050610621
42	19.014978092209528	25.12209838307041	28.749572720398586	27.113350804321477
43	20.669974415015382	24.695595653660103	28.280083073512806	26.354346857811706
44	19.613299012854643	25.8905542722781	27.764110585139996	26.732036129727266
45	19.66521995835966	25.382997897266446	26.66108182015931	28.290700324214583
46	21.56026972995929	26.646062294776314	27.09017412291151	24.703493852352885
47	18.749935260666454	25.59068167928652	30.484198423467753	25.175184636579278
48	19.163360644700177	24.426797940771277	29.321091556955075	27.088749857573468
49	19.89219606177686	23.24090282884992	30.918728830237928	25.94817227913529
50	19.262929739696087	23.68462622098383	29.971333423105207	27.081110616214872
51	18.291580779150827	23.90862431505785	27.608088791290747	30.191706114500576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	332.0
1	747.5
2	1163.0
3	2086.0
4	3009.0
5	2116.5
6	1224.0
7	1165.5
8	1107.0
9	1098.5
10	1090.0
11	1046.0
12	1002.0
13	1026.5
14	1051.0
15	1119.0
16	1187.0
17	1131.0
18	1075.0
19	1220.0
20	1365.0
21	1721.5
22	2078.0
23	2214.0
24	2350.0
25	3187.0
26	5358.5
27	6693.0
28	7859.5
29	9026.0
30	11277.5
31	13529.0
32	15198.0
33	16867.0
34	18287.0
35	19707.0
36	21355.0
37	23003.0
38	25953.0
39	28903.0
40	32849.5
41	36796.0
42	41398.5
43	46001.0
44	51831.0
45	57661.0
46	72156.5
47	86652.0
48	86555.0
49	86458.0
50	83633.0
51	80808.0
52	68802.0
53	56796.0
54	48492.5
55	40189.0
56	35579.5
57	30970.0
58	29681.5
59	28393.0
60	26979.5
61	25566.0
62	22313.0
63	19060.0
64	16768.0
65	14476.0
66	11514.0
67	8552.0
68	7034.0
69	5516.0
70	4393.0
71	3270.0
72	2690.0
73	2110.0
74	1904.0
75	1223.5
76	749.0
77	600.5
78	452.0
79	318.0
80	184.0
81	127.0
82	70.0
83	85.0
84	100.0
85	53.0
86	6.0
87	5.0
88	4.0
89	4.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	772328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.483670425545608
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.72677492285123	16.582000361961967
2	12.941599813662261	6.337157292340184
3	5.256296345122358	3.860802821189272
4	2.664273082845924	2.6092473633620785
5	1.6188454598932245	1.9817639354958259
6	1.0420471053328126	1.5307882736917435
7	0.6892499981210014	1.181275885636177
8	0.521948166119731	1.022336550279474
9	0.3916608911098055	0.8630366558857185
>10	5.77663863641095	37.39555422925201
>50	1.2861028446737384	19.367469776740602
>100	0.0754642118597208	3.3856381368162416
>500	0.0042816574104806125	0.7254283672800523
>1k	0.0048168645867906895	3.157500350068667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	4501	0.5827834805937374	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	4098	0.5306035777545292	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	3864	0.5003055696543438	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3723	0.48204907759397564	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	2224	0.28796055561885625	Illumina PCR Primer Index 11 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1763	0.2282708900881491	Illumina PCR Primer Index 11 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1615	0.20910804735811728	No Hit
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1279	0.1656032152142613	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	1029	0.13323354844055893	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	922	0.11937933106141432	No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	838	0.10850312302545034	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	798	0.10332397634165795	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5895733418961894E-4	0.0	0.0	0.2395355341253975	0.0
2	2.5895733418961894E-4	0.0	0.0	1.32858060305984	0.0
3	2.5895733418961894E-4	0.0	0.0	1.7505515791218238	0.0
4	2.5895733418961894E-4	0.0	0.0	2.4085621652976457	0.0
5	2.5895733418961894E-4	0.0	0.0	4.500807946882672	0.0
6	2.5895733418961894E-4	0.0	0.0	5.425156151272517	0.0
7	2.5895733418961894E-4	0.0	0.0	6.4530872893382085	0.0
8	2.5895733418961894E-4	0.0	0.0	7.912700303497996	0.0
9	2.5895733418961894E-4	0.0	0.0	8.403683409121513	0.0
10	2.5895733418961894E-4	0.0	0.0	10.092473664039114	0.0
11	2.5895733418961894E-4	0.0	0.0	11.808842875047906	0.0
12	2.5895733418961894E-4	0.0	0.0	13.540102132772605	0.0
13	2.5895733418961894E-4	0.0	0.0	14.072906847867745	0.0
14	2.5895733418961894E-4	0.0	0.0	14.287194041909656	0.0
15	2.5895733418961894E-4	0.0	0.0	14.751116106110358	0.0
16	2.5895733418961894E-4	0.0	0.0	15.568644410146984	0.0
17	2.5895733418961894E-4	0.0	0.0	16.525491759977626	0.0
18	2.5895733418961894E-4	0.0	0.0	17.583720906143505	0.0
19	2.5895733418961894E-4	0.0	0.0	18.340653193979758	0.0
20	2.5895733418961894E-4	0.0	0.0	18.996592121482063	0.0
21	2.5895733418961894E-4	0.0	0.0	19.847266964294963	0.0
22	2.5895733418961894E-4	0.0	0.0	20.75827886597404	0.0
23	2.5895733418961894E-4	0.0	0.0	21.630317688857584	0.0
24	3.884360012844284E-4	0.0	0.0	22.334163723184968	0.0
25	5.179146683792379E-4	0.0	0.0	22.9420660651951	0.0
26	5.179146683792379E-4	0.0	0.0	23.442371634849444	0.0
27	5.179146683792379E-4	0.0	0.0	23.96947928859241	0.0
28	5.179146683792379E-4	0.0	0.0	24.53206409711936	0.0
29	5.179146683792379E-4	0.0	0.0	25.069141608228627	0.0
30	5.179146683792379E-4	0.0	0.0	25.668757315544692	0.0
31	5.179146683792379E-4	0.0	0.0	26.23250743207549	0.0
32	5.179146683792379E-4	0.0	0.0	26.76984390051895	0.0
33	5.179146683792379E-4	0.0	0.0	27.309640463637212	0.0
34	5.179146683792379E-4	0.0	0.0	27.831568970696388	0.0
35	6.473933354740473E-4	0.0	0.0	28.419661076641013	0.0
36	6.473933354740473E-4	0.0	0.0	28.966061051781107	0.0
37	6.473933354740473E-4	0.0	0.0	29.4951108855305	0.0
38	6.473933354740473E-4	0.0	0.0	30.02843351529402	0.0
39	6.473933354740473E-4	0.0	0.0	30.5327529236283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGC	20	7.0318463E-4	45.000004	20
AACGTCA	20	7.0318463E-4	45.000004	35
CGAACCT	20	7.0318463E-4	45.000004	44
CGAACCG	20	7.0318463E-4	45.000004	14
ACCGAAC	20	7.0318463E-4	45.000004	12
CACGAGA	20	7.0318463E-4	45.000004	37
CGCTATA	20	7.0318463E-4	45.000004	20
ATAAGCG	20	7.0318463E-4	45.000004	33
CGTAAGT	20	7.0318463E-4	45.000004	22
TTTGCGC	20	7.0318463E-4	45.000004	13
ACGAGTC	20	7.0318463E-4	45.000004	24
TACGTAG	20	7.0318463E-4	45.000004	1
ACGAACC	20	7.0318463E-4	45.000004	43
GTAATCG	25	3.889613E-5	45.0	1
GCCGATA	25	3.889613E-5	45.0	9
TCGTACA	25	3.889613E-5	45.0	24
CGTCCGA	25	3.889613E-5	45.0	28
TAATGCG	35	1.2112287E-7	45.0	1
CGGACGG	50	2.1827873E-11	45.0	2
ATAGGCG	30	2.1645737E-6	44.999996	1
>>END_MODULE