FastQCFastQC Report
Sat 18 Jun 2016
SRR3549770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464164
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.21867271050749304No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC6710.14456097413845106Illumina Single End Adapter 2 (95% over 22bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA5540.11935436612921296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8871993E-545.01
AATCACG253.8871993E-545.022
TAGCGAA253.8871993E-545.01
ATCACGT207.028936E-445.011
ATCCGTA207.028936E-445.031
CCGTACC207.028936E-445.044
ACGGCAT253.8871993E-545.038
CGAATAT207.028936E-445.014
CGTACCG207.028936E-445.045
CGTAACA207.028936E-445.034
ACGTAAG207.028936E-445.01
CGGTCAA207.028936E-445.033
CGACTCG207.028936E-445.043
CGATAGT207.028936E-445.031
AGTACGG551.8189894E-1245.02
TAGTACG207.028936E-445.026
AACGTCG302.1626947E-644.9999961
TAGGGCG1300.041.538465
ACGGGAC556.002665E-1140.9090925
CGTTAGG451.924127E-840.02