##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549770_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464164
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.432360114097605	33.0	31.0	34.0	31.0	34.0
2	32.54186020458286	34.0	31.0	34.0	31.0	34.0
3	32.56483268844632	34.0	31.0	34.0	31.0	34.0
4	36.03109892193277	37.0	35.0	37.0	35.0	37.0
5	36.057901948449256	37.0	35.0	37.0	35.0	37.0
6	36.08594160684586	37.0	36.0	37.0	35.0	37.0
7	36.20923423617514	37.0	37.0	37.0	35.0	37.0
8	35.92864375522445	37.0	37.0	37.0	35.0	37.0
9	37.80174248756905	39.0	38.0	39.0	35.0	39.0
10	37.63296378004326	39.0	37.0	39.0	35.0	39.0
11	37.53146732620367	39.0	37.0	39.0	35.0	39.0
12	37.32763204384657	39.0	37.0	39.0	35.0	39.0
13	37.248942184227985	39.0	37.0	39.0	34.0	39.0
14	38.49176799579459	40.0	38.0	41.0	35.0	41.0
15	38.579258624107	40.0	38.0	41.0	35.0	41.0
16	38.634926448410475	40.0	38.0	41.0	35.0	41.0
17	38.639302057031564	40.0	38.0	41.0	35.0	41.0
18	38.63206539068088	40.0	38.0	41.0	35.0	41.0
19	38.623803224722295	40.0	38.0	41.0	35.0	41.0
20	38.569186752958004	40.0	37.0	41.0	35.0	41.0
21	38.48927965115778	40.0	37.0	41.0	35.0	41.0
22	38.48358769745176	40.0	37.0	41.0	35.0	41.0
23	38.43290733447661	40.0	37.0	41.0	35.0	41.0
24	38.36177730284985	40.0	37.0	41.0	34.0	41.0
25	38.27854809937867	40.0	37.0	41.0	34.0	41.0
26	38.264503494454544	40.0	37.0	41.0	34.0	41.0
27	38.21404503580631	40.0	36.0	41.0	34.0	41.0
28	38.15788169698641	40.0	36.0	41.0	34.0	41.0
29	38.11964090278436	40.0	36.0	41.0	34.0	41.0
30	38.038410992666385	40.0	36.0	41.0	34.0	41.0
31	37.99092777552762	40.0	36.0	41.0	34.0	41.0
32	37.930608578002605	40.0	36.0	41.0	34.0	41.0
33	37.877517429184515	40.0	36.0	41.0	34.0	41.0
34	37.81146965296749	40.0	36.0	41.0	34.0	41.0
35	37.74213209124361	40.0	35.0	41.0	33.0	41.0
36	37.66423290044036	40.0	35.0	41.0	33.0	41.0
37	37.605232202411216	40.0	35.0	41.0	33.0	41.0
38	37.51367404624228	40.0	35.0	41.0	33.0	41.0
39	37.44985823976008	40.0	35.0	41.0	33.0	41.0
40	37.35566954783223	40.0	35.0	41.0	33.0	41.0
41	37.28129281891745	39.0	35.0	41.0	33.0	41.0
42	37.252184572694134	39.0	35.0	41.0	33.0	41.0
43	37.18371092975759	39.0	35.0	41.0	33.0	41.0
44	37.11543764703855	39.0	35.0	41.0	33.0	41.0
45	37.044596306477885	39.0	35.0	41.0	33.0	41.0
46	36.918989839798	39.0	35.0	41.0	33.0	41.0
47	36.84639049990952	39.0	35.0	41.0	32.0	41.0
48	36.799068863591316	39.0	35.0	41.0	32.0	41.0
49	36.68683051680009	39.0	35.0	41.0	32.0	41.0
50	36.619625821907775	38.0	35.0	41.0	32.0	41.0
51	35.774392240673556	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	10.0
10	5.0
11	7.0
12	10.0
13	17.0
14	16.0
15	23.0
16	36.0
17	54.0
18	103.0
19	159.0
20	301.0
21	407.0
22	630.0
23	891.0
24	1372.0
25	2028.0
26	2611.0
27	3139.0
28	3625.0
29	4158.0
30	4847.0
31	6317.0
32	8451.0
33	12268.0
34	26979.0
35	42087.0
36	29876.0
37	45465.0
38	80188.0
39	187729.0
40	350.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13367689006472	21.43035651192251	23.984195241337115	11.451771356675659
2	32.76880585310364	24.131341508604716	24.39310243793142	18.70675020036022
3	33.88931498349721	23.614067441680096	25.28696753733594	17.20965003748675
4	29.361820391068672	26.261838488120574	24.84789858756819	19.52844253324256
5	27.00446393946967	29.978412802371572	23.346058720624608	19.671064537534146
6	27.270318249584196	34.43351918718384	22.653631044199894	15.642531519032065
7	81.36283727303281	6.665101128049569	7.460940529640387	4.511121069277238
8	82.73541248351876	5.529511121069277	7.346541308675382	4.388535086736584
9	76.73688610060238	7.8648925810704835	9.449030945958755	5.9491903723683865
10	46.064752975241504	22.956541222498945	16.532518678742857	14.446187123516689
11	33.749493713428876	26.181048077834557	21.28687274325454	18.782585465482025
12	30.555794934548995	23.38505355865599	25.916270973190507	20.1428805336045
13	25.831387182116668	24.92244120612542	27.858687877560516	21.387483734197396
14	22.08077317499849	27.153333735490044	28.43326927551469	22.332623813996776
15	21.385975646538725	25.928766556648082	31.855335614136383	20.829922182676814
16	24.47884799338165	24.879137546212114	29.8189863927405	20.82302806766574
17	24.661972923363294	24.9726389810498	26.888556630845994	23.47683146474091
18	25.1964822778156	25.75512103480666	27.241880025163518	21.806516662214218
19	26.47124723158194	26.779758878327485	25.387793969372897	21.361199920717677
20	28.0178988460975	26.148085590437866	26.218965710395466	19.615049853069173
21	26.742703010142964	26.088408407373255	26.993261002576673	20.1756275799071
22	25.888263630958026	24.093639317137907	26.301695090528348	23.716401961375723
23	25.18377125326393	25.181832283417066	27.214949888401513	22.419446574917487
24	25.14154479882111	24.31274290983359	28.228384795029342	22.317327496315958
25	24.546281055833713	26.036702544790202	26.238355408864106	23.178660990511972
26	23.01018605492886	27.47606449444593	27.030963194043483	22.482786256581726
27	23.790944579932955	26.336811988866003	27.724683517032773	22.14755991416827
28	22.07646435311657	27.420911574357337	28.185727458398325	22.316896614127764
29	24.04064080799028	26.02851578321455	27.553407847226413	22.377435561568756
30	24.401720081695263	26.555053817185303	26.496669280685275	22.54655682043416
31	24.74728759662533	27.83735920924501	24.733068484414993	22.68228470971467
32	25.775587938745787	27.524323299523445	24.55640678725623	22.14368197447454
33	25.417093958169957	27.88044742806422	23.706707112141398	22.995751501624426
34	22.99596694271852	27.624503408278105	26.502701631319965	22.876828017683405
35	24.474108289311538	27.019760257150487	25.394903525478064	23.11122792805991
36	25.534940236640498	28.931799967252953	24.38103773666204	21.152222059444505
37	25.182478606699355	28.65345007368085	24.892279452951975	21.271791866667815
38	24.103765048560422	28.4382244206789	24.274825277272686	23.18318525348799
39	25.201221981885713	26.145931179496902	25.84366732448014	22.809179514137245
40	24.59303177325256	25.986504769865824	27.162382261442076	22.258081195439544
41	23.156039675631888	26.98873673960066	26.061693711705345	23.79352987306211
42	23.227566118871778	26.85839487767255	25.972716539843677	23.941322463612
43	23.71424755043476	25.860256288725537	26.606544238674264	23.81895192216544
44	23.32128299480356	26.180832636740465	26.104566489430457	24.393317879025517
45	23.671590213803743	25.82557027257607	25.887401866581637	24.615437647038547
46	24.26599219241475	26.91053162244379	25.36280280245775	23.460673382683705
47	23.32774622762644	26.782559612550738	27.153764617678235	22.735929542144586
48	23.24867934609319	25.452641738695807	27.43900862626141	23.859670288949594
49	23.246955817340424	25.85788643669048	27.67814824070803	23.21700950526107
50	22.610327384286588	25.912177592402685	27.256530019562053	24.220965003748677
51	22.596108272076247	25.89774303909825	25.257667548538876	26.24848114028662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	522.0
2	862.0
3	1368.0
4	1874.0
5	1335.5
6	797.0
7	746.5
8	696.0
9	716.0
10	736.0
11	780.0
12	824.0
13	809.0
14	794.0
15	808.0
16	822.0
17	863.5
18	905.0
19	917.0
20	929.0
21	1391.5
22	1854.0
23	2087.0
24	2320.0
25	2194.5
26	2738.0
27	3407.0
28	4270.5
29	5134.0
30	5892.5
31	6651.0
32	7374.0
33	8097.0
34	9165.0
35	10233.0
36	11496.5
37	12760.0
38	13752.5
39	14745.0
40	16727.0
41	18709.0
42	21189.0
43	23669.0
44	26500.0
45	29331.0
46	32175.5
47	35020.0
48	37817.0
49	40614.0
50	39355.0
51	38096.0
52	35764.5
53	33433.0
54	30889.5
55	28346.0
56	26795.0
57	25244.0
58	24670.0
59	24096.0
60	23972.0
61	23848.0
62	21830.0
63	19812.0
64	17104.5
65	14397.0
66	12385.0
67	10373.0
68	9424.0
69	8475.0
70	6951.0
71	5427.0
72	4523.0
73	3619.0
74	2997.0
75	1754.0
76	1133.0
77	972.5
78	812.0
79	541.0
80	270.0
81	284.5
82	299.0
83	176.0
84	53.0
85	35.5
86	18.0
87	10.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	464164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.15204031487636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15095389037975	19.861976491032525
2	8.946196279081546	4.85814964068838
3	3.0238846275304385	2.46313911942724
4	1.6413293194153096	1.782617594030122
5	1.1074160975818879	1.5034303263443234
6	0.780875699358505	1.2721421081933637
7	0.6594599905538774	1.2533978974696802
8	0.62831593953257	1.3648047776534205
9	0.5350151374524748	1.3074077323060842
>10	9.282568683987034	58.65108693194595
>50	0.20092827598660315	3.3889746199561186
>100	0.04066405585443159	1.8079294142873095
>500	0.001594668857036533	0.2652033927076942
>1k	7.973344285182665E-4	0.21973995395780377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1015	0.21867271050749304	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	671	0.14456097413845106	Illumina Single End Adapter 2 (95% over 22bp)
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	554	0.11935436612921296	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1544109409605227E-4	0.0	0.0	0.08811540748528537	0.0
2	2.1544109409605227E-4	0.0	0.0	0.4444549771201558	0.0
3	2.1544109409605227E-4	0.0	0.0	0.6262872605372239	0.0
4	2.1544109409605227E-4	0.0	0.0	0.9162709731905102	0.0
5	2.1544109409605227E-4	0.0	0.0	1.7116794925931351	0.0
6	2.1544109409605227E-4	0.0	0.0	2.185865340698546	0.0
7	2.1544109409605227E-4	0.0	0.0	2.641523254711697	0.0
8	2.1544109409605227E-4	0.0	0.0	3.4199119276807335	0.0
9	2.1544109409605227E-4	0.0	0.0	3.7469515085185408	0.0
10	2.1544109409605227E-4	0.0	0.0	4.454460061529977	0.0
11	2.1544109409605227E-4	0.0	0.0	5.3996001413293575	0.0
12	2.1544109409605227E-4	0.0	0.0	6.119819718892461	0.0
13	2.1544109409605227E-4	0.0	0.0	6.412819606863091	0.0
14	2.1544109409605227E-4	0.0	0.0	6.52312544704027	0.0
15	2.1544109409605227E-4	0.0	0.0	6.76269594367508	0.0
16	2.1544109409605227E-4	0.0	0.0	7.242698701321085	0.0
17	2.1544109409605227E-4	0.0	0.0	7.789919080325057	0.0
18	2.1544109409605227E-4	0.0	0.0	8.387552675347507	0.0
19	2.1544109409605227E-4	0.0	0.0	8.792581932248085	0.0
20	2.1544109409605227E-4	0.0	0.0	9.17542075645677	0.0
21	2.1544109409605227E-4	0.0	0.0	9.659086012702407	0.0
22	2.1544109409605227E-4	0.0	0.0	10.135426271748779	0.0
23	2.1544109409605227E-4	0.0	0.0	10.599055506243483	0.0
24	2.1544109409605227E-4	0.0	0.0	10.996113442662507	0.0
25	2.1544109409605227E-4	0.0	0.0	11.345343456192207	0.0
26	2.1544109409605227E-4	0.0	0.0	11.67216759593592	0.0
27	2.1544109409605227E-4	0.0	0.0	11.985849828939772	0.0
28	2.1544109409605227E-4	0.0	0.0	12.334002636998992	0.0
29	2.1544109409605227E-4	0.0	0.0	12.66965986160064	0.0
30	2.1544109409605227E-4	0.0	0.0	13.053145009091613	0.0
31	4.3088218819210453E-4	0.0	0.0	13.408838255444197	0.0
32	4.3088218819210453E-4	0.0	0.0	13.798140312475763	0.0
33	4.3088218819210453E-4	0.0	0.0	14.154264441016537	0.0
34	4.3088218819210453E-4	0.0	0.0	14.49789298609974	0.0
35	4.3088218819210453E-4	0.0	0.0	14.875345782956025	0.0
36	4.3088218819210453E-4	0.0	0.0	15.234486086814144	0.0
37	4.3088218819210453E-4	0.0	0.0	15.58673227566119	0.0
38	4.3088218819210453E-4	0.0	0.0	15.953628458906765	0.0
39	4.3088218819210453E-4	0.0	0.0	16.323325376375593	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.8871993E-5	45.0	1
AATCACG	25	3.8871993E-5	45.0	22
TAGCGAA	25	3.8871993E-5	45.0	1
ATCACGT	20	7.028936E-4	45.0	11
ATCCGTA	20	7.028936E-4	45.0	31
CCGTACC	20	7.028936E-4	45.0	44
ACGGCAT	25	3.8871993E-5	45.0	38
CGAATAT	20	7.028936E-4	45.0	14
CGTACCG	20	7.028936E-4	45.0	45
CGTAACA	20	7.028936E-4	45.0	34
ACGTAAG	20	7.028936E-4	45.0	1
CGGTCAA	20	7.028936E-4	45.0	33
CGACTCG	20	7.028936E-4	45.0	43
CGATAGT	20	7.028936E-4	45.0	31
AGTACGG	55	1.8189894E-12	45.0	2
TAGTACG	20	7.028936E-4	45.0	26
AACGTCG	30	2.1626947E-6	44.999996	1
TAGGGCG	130	0.0	41.53846	5
ACGGGAC	55	6.002665E-11	40.909092	5
CGTTAGG	45	1.924127E-8	40.0	2
>>END_MODULE