FastQCFastQC Report
Sat 18 Jun 2016
SRR3549764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446485
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8920.19978274746072097No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.11377761850902045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT253.8869606E-545.09
CCGATGT207.028647E-445.011
CTAACGG253.8869606E-545.02
CATACGG253.8869606E-545.02
TACTCGT207.028647E-445.031
ATGCGAT207.028647E-445.014
AGTACGG650.044.9999962
AGGCGAT1800.042.57
CGTTTTA2050.041.707321
TTTCGAC451.9237632E-840.024
ATAGCGG403.4518962E-739.3752
ACACGCG1200.039.37499636
CTATGCG356.2397066E-638.571431
CGAACGG356.2397066E-638.571432
TAAGGCG356.2397066E-638.571435
CTACGAA900.037.511
TACGAAT900.037.512
TATGCGG601.546141E-1037.4999962
TCAGCGT301.1388041E-437.49999633
CGCTAGA301.1388041E-437.49999612