FastQCFastQC Report
Sat 18 Jun 2016
SRR3549760_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549760_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623202
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.23635996033388854No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.1335040644927327No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA253.8887418E-545.00000427
TACTCGA207.0307986E-445.01
AATCGGT302.163897E-644.99999633
CGTTTTA3300.042.9545441
CTACGAA1950.041.5384611
CGAATAT1900.041.4473714
TACGAAT1850.041.35135312
AGGCGAT2600.039.8076937
TCGCAAC403.4548248E-739.37516
ACGGGAT3900.038.6538475
GCACGTA356.2436866E-638.5714261
TAAACGG356.2436866E-638.5714262
GTTAACG356.2436866E-638.5714261
GAGCGTA356.2436866E-638.5714269
AAACACG659.094947E-1238.07692740
CGTTTTT6400.037.6171881
AAATCGT301.1393225E-437.49999623
CATGCGG301.1393225E-437.4999962
TAAGCGA301.1393225E-437.4999961
AAGCTAC2050.037.3170748
Produced by FastQC (version 0.11.5)