##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549760_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623202
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03184681692292	33.0	31.0	34.0	30.0	34.0
2	32.22961736323054	33.0	31.0	34.0	30.0	34.0
3	32.1333018828566	33.0	31.0	34.0	30.0	34.0
4	35.86642854162856	37.0	35.0	37.0	35.0	37.0
5	32.15302101084399	37.0	35.0	37.0	0.0	37.0
6	34.009374167605365	37.0	35.0	37.0	19.0	37.0
7	20.678235949178596	32.0	0.0	37.0	0.0	37.0
8	28.06333740905838	35.0	17.0	37.0	17.0	37.0
9	34.82477270612097	37.0	32.0	39.0	32.0	39.0
10	36.318216244492156	37.0	35.0	39.0	32.0	39.0
11	36.78151867291825	39.0	37.0	39.0	33.0	39.0
12	37.07423435739937	39.0	37.0	39.0	34.0	39.0
13	36.825345554090006	39.0	37.0	39.0	33.0	39.0
14	38.125604539138195	40.0	37.0	41.0	33.0	41.0
15	38.30934271712864	40.0	38.0	41.0	34.0	41.0
16	38.41402145692729	40.0	38.0	41.0	34.0	41.0
17	38.39558281263539	40.0	38.0	41.0	34.0	41.0
18	38.31202242611545	40.0	37.0	41.0	34.0	41.0
19	38.27765796643785	40.0	37.0	41.0	34.0	41.0
20	38.27938774265808	40.0	37.0	41.0	34.0	41.0
21	38.19294867474751	40.0	37.0	41.0	34.0	41.0
22	38.1697282742995	40.0	37.0	41.0	34.0	41.0
23	38.128703052942704	40.0	37.0	41.0	34.0	41.0
24	38.03322839143649	40.0	37.0	41.0	34.0	41.0
25	37.8935722285872	40.0	36.0	41.0	33.0	41.0
26	37.753547004021165	40.0	36.0	41.0	33.0	41.0
27	37.72396911434816	40.0	36.0	41.0	33.0	41.0
28	37.62553393602716	40.0	36.0	41.0	33.0	41.0
29	37.6014502520852	40.0	36.0	41.0	33.0	41.0
30	37.45363461606349	40.0	36.0	41.0	33.0	41.0
31	37.32746043818858	40.0	36.0	41.0	32.0	41.0
32	37.256210666846385	40.0	36.0	41.0	32.0	41.0
33	37.12429517235182	40.0	35.0	41.0	32.0	41.0
34	36.97641374706756	39.0	35.0	41.0	31.0	41.0
35	36.85249245028097	39.0	35.0	41.0	31.0	41.0
36	36.71327595225946	39.0	35.0	41.0	30.0	41.0
37	36.65486792404389	39.0	35.0	41.0	30.0	41.0
38	36.50876922731313	39.0	35.0	41.0	30.0	41.0
39	36.42259331645277	39.0	35.0	41.0	30.0	41.0
40	36.30822269504912	39.0	35.0	41.0	30.0	41.0
41	36.16661531894955	39.0	35.0	40.0	29.0	41.0
42	36.06494362983431	39.0	35.0	40.0	29.0	41.0
43	36.02054229607735	39.0	35.0	40.0	29.0	41.0
44	35.880069383602745	39.0	35.0	40.0	28.0	41.0
45	35.794801685488814	39.0	35.0	40.0	28.0	41.0
46	35.664713848800226	38.0	35.0	40.0	27.0	41.0
47	35.58769708697982	38.0	35.0	40.0	27.0	41.0
48	35.46792853681471	38.0	34.0	40.0	27.0	41.0
49	35.35139810206	38.0	34.0	40.0	27.0	41.0
50	35.20819894672995	38.0	34.0	40.0	26.0	41.0
51	33.339929910366145	36.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	23.0
9	45.0
10	35.0
11	36.0
12	14.0
13	21.0
14	18.0
15	33.0
16	77.0
17	115.0
18	242.0
19	365.0
20	667.0
21	1075.0
22	1679.0
23	2582.0
24	3747.0
25	5160.0
26	6578.0
27	7425.0
28	8474.0
29	9755.0
30	12091.0
31	15735.0
32	20995.0
33	30405.0
34	42241.0
35	53181.0
36	71518.0
37	121411.0
38	125695.0
39	81747.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.7883768023851	20.23822131507922	25.42594535961053	12.547456522925152
2	32.52155801810649	24.295332813437696	26.0636198215025	17.119489346953316
3	32.516583708011204	23.09540084916287	27.407967240156484	16.98004820266944
4	28.917108738418683	26.230981286966344	26.430435075625557	18.421474898989413
5	23.008430653303424	37.60385878094101	22.617867080015788	16.769843485739777
6	27.85581561034785	34.373926912943155	23.91921078558798	13.851046691121017
7	49.690630004396645	43.379674648027446	4.446070455486343	2.4836248920895634
8	86.05347864737276	4.61038315024663	6.511853299572209	2.824284902808399
9	78.66710954072677	8.556776133581085	8.46306654985061	4.31304777584154
10	39.10481673678839	34.984483361735045	13.835802837603218	12.074897063873351
11	34.075789230458184	25.728575967342852	23.227300297495837	16.96833450470313
12	31.87265124309614	22.82470210300994	26.582231764339653	18.72041488955427
13	26.516923886637077	24.394337630495407	28.258413804833744	20.83032467803377
14	21.875250721274963	26.637751483467643	29.477601163025792	22.009396632231603
15	20.849419610335012	26.207232967801776	32.66292470178209	20.28042272008113
16	25.606946062432407	25.188462167964804	29.92175891604969	19.282832853553103
17	25.33737054759131	24.19857445900366	28.072920176764516	22.391134816640513
18	25.21140817904949	25.08480396404376	28.696634478066503	21.00715337884025
19	25.665995937111884	26.093786605306146	27.106299402120023	21.133918055461955
20	27.847471606317054	25.357428249588416	27.304469497851418	19.490630646243112
21	26.876197444809225	25.599725289713447	27.62699734596487	19.897079919512453
22	25.2993411446048	24.174986601455064	28.530877628762426	21.99479462517771
23	24.10326025911342	25.84940356417341	28.67128154274216	21.37605463397101
24	23.777202255448472	25.11128013067994	29.38966819747048	21.721849416401103
25	23.97152127239643	26.74076784092477	26.913585001331832	22.374125885346967
26	23.425951778075166	27.814416513425822	26.086565832587187	22.673065875911824
27	23.09716592693862	27.313134425114168	28.053183397999366	21.53651624994785
28	21.23228102605576	28.027990924291004	28.76723758909631	21.97249046055693
29	22.843636573695207	26.901710841749544	28.589606580209946	21.6650460043453
30	23.908299395701555	25.74542443702042	28.48851576214454	21.85776040513349
31	24.755697189675256	26.945195939679266	26.918880234659063	21.380226635986403
32	25.573890969541175	27.067467691053622	27.107422633431856	20.251218705973344
33	25.416478124267893	27.07886046578798	26.453702009942205	21.050959400001926
34	23.22152367932067	26.890478528631167	28.741884653771972	21.146113138276192
35	23.874923379578373	27.585598249042846	27.286497796862015	21.25298057451677
36	24.73210933212666	28.435884352104136	26.436532617032682	20.395473698736524
37	24.446487655687882	28.986428156520677	26.273503615200205	20.29358057259123
38	23.684134518181907	27.564256854117925	26.880369446824627	21.87123918087554
39	24.232431859974778	25.784577071318772	27.559282544022643	22.42370852468381
40	25.486760312065748	25.79966046322059	27.76226648823335	20.951312736480308
41	22.287797535951427	26.17465925975847	28.41341972586737	23.124123478422725
42	22.264691063250762	27.024784901203784	28.039544160641334	22.670979874904123
43	23.14321841072397	26.481622331122175	28.32997968555942	22.04517957259444
44	23.14016964002041	27.006331815366448	27.70803046203318	22.145468082579967
45	23.12139563095112	26.813296491346307	26.641442100635103	23.42386577706747
46	22.521429648813708	27.757613101370023	26.772860164120143	22.94809708569613
47	22.9843614109069	27.415990320955324	28.256809188673977	21.3428390794638
48	22.860645504988753	26.700491975314584	28.601480739792233	21.83738177990443
49	23.10406577642562	26.32677687170452	28.05125785860764	22.517899493262217
50	21.317486144139462	27.297569648364416	28.371057859249486	23.013886348246636
51	22.023517254437568	26.675139039990242	27.703697998401804	23.597645707170386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	353.0
1	997.0
2	1641.0
3	3267.0
4	4893.0
5	3441.0
6	1989.0
7	1785.5
8	1582.0
9	1602.0
10	1622.0
11	1554.5
12	1487.0
13	1474.0
14	1461.0
15	1447.5
16	1434.0
17	1479.5
18	1525.0
19	1702.0
20	1879.0
21	2009.0
22	2139.0
23	2470.5
24	2802.0
25	3829.5
26	5878.0
27	6899.0
28	8040.5
29	9182.0
30	10727.0
31	12272.0
32	14888.5
33	17505.0
34	19246.5
35	20988.0
36	21914.5
37	22841.0
38	24916.5
39	26992.0
40	29065.0
41	31138.0
42	33743.0
43	36348.0
44	37962.5
45	39577.0
46	41200.5
47	42824.0
48	42874.0
49	42924.0
50	42650.0
51	42376.0
52	40742.0
53	39108.0
54	37983.5
55	36859.0
56	35920.0
57	34981.0
58	33911.0
59	32841.0
60	31308.0
61	29775.0
62	26761.5
63	23748.0
64	20031.5
65	16315.0
66	13271.5
67	10228.0
68	8457.5
69	6687.0
70	5550.0
71	4413.0
72	3555.0
73	2697.0
74	2261.0
75	1446.5
76	1068.0
77	802.0
78	536.0
79	396.5
80	257.0
81	196.5
82	136.0
83	107.5
84	79.0
85	63.5
86	48.0
87	44.0
88	40.0
89	26.5
90	13.0
91	10.0
92	7.0
93	7.0
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	623202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.6770206734045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.43267353480068	24.770722969228377
2	7.43936779473329	5.159502216300518
3	3.7109170678091323	3.8605064363312067
4	2.523907764946765	3.5008640697130042
5	1.805721932971921	3.130852840004362
6	1.4668270999744062	3.051911620207347
7	1.3331876574421289	3.2361683171053897
8	1.0969025350764356	3.0429849508444287
9	1.0441308314996252	3.2586611786686372
>10	8.089271248959527	44.56708707376476
>50	0.03655925281057544	0.8389832026567401
>100	0.019030843928552726	1.0798587360934908
>500	0.0010016233646606698	0.24608553056360188
>1k	5.008116823303349E-4	0.2558108585181267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1473	0.23635996033388854	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	832	0.1335040644927327	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6046161597684219E-4	0.0	0.0	0.026636628252155802	0.0
2	1.6046161597684219E-4	0.0	0.0	0.14200853013950532	0.0
3	1.6046161597684219E-4	0.0	0.0	0.18661685938106745	0.0
4	1.6046161597684219E-4	0.0	0.0	0.22689272499125485	0.0
5	1.6046161597684219E-4	0.0	0.0	0.3693826399786907	0.0
6	1.6046161597684219E-4	0.0	0.0	0.47865700045892023	0.0
7	1.6046161597684219E-4	0.0	0.0	0.5895359770989181	0.0
8	1.6046161597684219E-4	0.0	0.0	0.8435467151902594	0.0
9	1.6046161597684219E-4	0.0	0.0	0.9387004534645268	0.0
10	1.6046161597684219E-4	0.0	0.0	1.2105224309292975	0.0
11	1.6046161597684219E-4	0.0	0.0	1.594186154729927	0.0
12	1.6046161597684219E-4	0.0	0.0	1.9130233856759125	0.0
13	1.6046161597684219E-4	0.0	0.0	2.0511808370319735	0.0
14	1.6046161597684219E-4	0.0	0.0	2.11488409857478	0.0
15	1.6046161597684219E-4	0.0	0.0	2.2211096883514494	0.0
16	1.6046161597684219E-4	0.0	0.0	2.4313144052811126	0.0
17	1.6046161597684219E-4	0.0	0.0	2.693027300939342	0.0
18	1.6046161597684219E-4	0.0	0.0	2.9752792834426076	0.0
19	1.6046161597684219E-4	0.0	0.0	3.1484173670816205	0.0
20	1.6046161597684219E-4	0.0	0.0	3.345945616349113	0.0
21	1.6046161597684219E-4	0.0	0.0	3.5750848039640437	0.0
22	1.6046161597684219E-4	0.0	0.0	3.8204306147926355	0.0
23	1.6046161597684219E-4	0.0	0.0	4.071553043796394	0.0
24	1.6046161597684219E-4	0.0	0.0	4.277746220326636	0.0
25	1.6046161597684219E-4	0.0	0.0	4.461153847388166	0.0
26	1.6046161597684219E-4	0.0	0.0	4.631082698707642	0.0
27	1.6046161597684219E-4	0.0	0.0	4.839201414629606	0.0
28	1.6046161597684219E-4	0.0	0.0	5.026781043706535	0.0
29	1.6046161597684219E-4	0.0	0.0	5.227839448525518	0.0
30	1.6046161597684219E-4	0.0	0.0	5.4754317219777855	0.0
31	1.6046161597684219E-4	0.0	0.0	5.677613358108607	0.0
32	1.6046161597684219E-4	0.0	0.0	5.894557462909297	0.0
33	1.6046161597684219E-4	0.0	0.0	6.100429716207586	0.0
34	1.6046161597684219E-4	0.0	0.0	6.316892436160346	0.0
35	1.6046161597684219E-4	0.0	0.0	6.544427007615508	0.0
36	1.6046161597684219E-4	0.0	0.0	6.775973119470091	0.0
37	1.6046161597684219E-4	0.0	0.0	7.014419080811679	0.0
38	1.6046161597684219E-4	0.0	0.0	7.2608881229521085	0.0
39	1.6046161597684219E-4	0.0	0.0	7.617754756884605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	25	3.8887418E-5	45.000004	27
TACTCGA	20	7.0307986E-4	45.0	1
AATCGGT	30	2.163897E-6	44.999996	33
CGTTTTA	330	0.0	42.954544	1
CTACGAA	195	0.0	41.53846	11
CGAATAT	190	0.0	41.44737	14
TACGAAT	185	0.0	41.351353	12
AGGCGAT	260	0.0	39.807693	7
TCGCAAC	40	3.4548248E-7	39.375	16
ACGGGAT	390	0.0	38.653847	5
GCACGTA	35	6.2436866E-6	38.571426	1
TAAACGG	35	6.2436866E-6	38.571426	2
GTTAACG	35	6.2436866E-6	38.571426	1
GAGCGTA	35	6.2436866E-6	38.571426	9
AAACACG	65	9.094947E-12	38.076927	40
CGTTTTT	640	0.0	37.617188	1
AAATCGT	30	1.1393225E-4	37.499996	23
CATGCGG	30	1.1393225E-4	37.499996	2
TAAGCGA	30	1.1393225E-4	37.499996	1
AAGCTAC	205	0.0	37.317074	8
>>END_MODULE