FastQCFastQC Report
Sat 18 Jun 2016
SRR3549757_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549757_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504409
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.4339732241097998No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.24860777662571448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGT551.8189894E-1245.00000428
TATCGCG351.2101191E-745.00000432
CTTCGTA207.029519E-445.044
CCGGATA207.029519E-445.021
GAGACCG253.8876824E-545.06
GTATCCG207.029519E-445.029
TCCCGAT207.029519E-445.039
CGCGTAA253.8876824E-545.031
TCGAATA207.029519E-445.01
TCGGTAC207.029519E-445.037
CGAATAT1200.044.99999614
GCTACGA1250.043.210
CGTTATT1850.042.5675661
AGGCGAT3550.041.8309867
CGTTTTA4950.041.3636361
ATGATCG603.6379788E-1241.24999627
TGTACGG603.6379788E-1241.2499962
TAGGCGA1050.040.7142876
CTACGAA1350.040.011
TACGAAT1350.040.012