##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549757_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504409
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8935050722727	31.0	31.0	34.0	30.0	34.0
2	32.08234190904603	33.0	31.0	34.0	30.0	34.0
3	31.893362330965545	33.0	31.0	34.0	30.0	34.0
4	35.701347517589895	37.0	35.0	37.0	35.0	37.0
5	31.988901863368813	37.0	35.0	37.0	0.0	37.0
6	33.89698637415272	37.0	35.0	37.0	19.0	37.0
7	20.903766586242515	32.0	0.0	37.0	0.0	37.0
8	28.184790517219163	35.0	17.0	37.0	17.0	37.0
9	34.865965912582844	37.0	32.0	39.0	32.0	39.0
10	36.166230182252896	37.0	35.0	39.0	32.0	39.0
11	36.631091039216194	38.0	35.0	39.0	32.0	39.0
12	36.99635216659497	39.0	37.0	39.0	33.0	39.0
13	36.81201564603328	39.0	37.0	39.0	33.0	39.0
14	38.201229557759675	40.0	37.0	41.0	33.0	41.0
15	38.35612568372095	40.0	38.0	41.0	34.0	41.0
16	38.4112020205825	40.0	38.0	41.0	34.0	41.0
17	38.36655967677024	40.0	38.0	41.0	34.0	41.0
18	38.267344555707766	40.0	37.0	41.0	34.0	41.0
19	38.20598760133146	40.0	37.0	41.0	34.0	41.0
20	38.17646790600485	40.0	37.0	41.0	34.0	41.0
21	38.09071011817791	40.0	37.0	41.0	34.0	41.0
22	38.06462017925929	40.0	37.0	41.0	34.0	41.0
23	37.99947859772526	40.0	37.0	41.0	34.0	41.0
24	37.86886633664348	40.0	36.0	41.0	33.0	41.0
25	37.71825641493312	40.0	36.0	41.0	33.0	41.0
26	37.59951745508109	40.0	36.0	41.0	33.0	41.0
27	37.54680229734204	39.0	36.0	41.0	33.0	41.0
28	37.47607596216562	40.0	36.0	41.0	33.0	41.0
29	37.47359583195383	40.0	36.0	41.0	33.0	41.0
30	37.29356335830646	39.0	36.0	41.0	32.0	41.0
31	37.179982910693504	39.0	35.0	41.0	32.0	41.0
32	37.0724768987072	39.0	35.0	41.0	31.0	41.0
33	36.9001088402467	39.0	35.0	41.0	31.0	41.0
34	36.7005644229187	39.0	35.0	41.0	30.0	41.0
35	36.54591214669048	39.0	35.0	41.0	30.0	41.0
36	36.335101078688126	39.0	35.0	41.0	30.0	41.0
37	36.27862508400921	39.0	35.0	41.0	30.0	41.0
38	36.16524288821175	39.0	35.0	41.0	29.0	41.0
39	36.09840427113711	39.0	35.0	41.0	29.0	41.0
40	36.0214389513272	39.0	35.0	41.0	28.0	41.0
41	35.91347894268342	39.0	35.0	40.0	28.0	41.0
42	35.78470645844939	39.0	35.0	40.0	27.0	41.0
43	35.683647595502855	39.0	35.0	40.0	27.0	41.0
44	35.553701460521125	39.0	35.0	40.0	26.0	41.0
45	35.4662149168631	38.0	35.0	40.0	26.0	41.0
46	35.32751992926375	38.0	34.0	40.0	25.0	41.0
47	35.31515099849527	38.0	34.0	40.0	25.0	41.0
48	35.19348385932844	38.0	34.0	40.0	24.0	41.0
49	35.054360647807634	38.0	34.0	40.0	24.0	41.0
50	34.922471645034086	38.0	34.0	40.0	24.0	41.0
51	32.985055778148286	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	24.0
10	16.0
11	26.0
12	19.0
13	14.0
14	15.0
15	26.0
16	44.0
17	81.0
18	192.0
19	341.0
20	632.0
21	1019.0
22	1635.0
23	2574.0
24	3927.0
25	5241.0
26	6447.0
27	7020.0
28	7654.0
29	8725.0
30	10272.0
31	13144.0
32	17111.0
33	24672.0
34	33908.0
35	43941.0
36	56589.0
37	95378.0
38	98942.0
39	64757.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.400944471649	20.08608093828619	25.539988382443614	13.972986207621197
2	33.00753951654312	23.934545180597492	26.693813948601235	16.36410135425815
3	31.214550097242515	23.01069172040943	29.424732706989765	16.35002547535829
4	27.295309956800928	25.923010889972225	28.814513618908467	17.96716553431838
5	21.786685011567993	37.34905602398054	24.734094752472696	16.13016421197877
6	25.905168226578034	34.06660071489605	26.38533412369724	13.64289693482868
7	49.238415650791325	42.46871090722013	5.714806833343576	2.578066608644969
8	84.77386406666018	4.275300401063423	8.369795146399053	2.5810403858773334
9	78.51921753973463	7.824602653798802	9.831307530198707	3.8248722762678695
10	36.581028490768404	33.909188773396195	16.92059420034139	12.589188535494014
11	29.811918502643685	25.226155758521358	27.99751788727005	16.9644078515649
12	27.441421544817796	23.659768164326962	30.510954404064954	18.387855886790284
13	23.45299152076985	25.9719790883985	31.66715899200847	18.907870398823174
14	20.555937740999862	28.025867896885266	31.765690144307495	19.652504217807373
15	20.23179602267208	26.345485508783543	34.62824810818205	18.794470360362325
16	23.87685390228961	25.512233128274875	32.195103576661005	18.415809392774516
17	23.25255893530845	24.64012339192996	30.444540045875467	21.66277762688612
18	23.54815239220553	25.32250614084999	31.09500425250144	20.034337214443042
19	23.51861287169737	27.06811337624824	29.45823726380774	19.95503648824664
20	25.089362005832566	26.25746170270554	29.745900648085183	18.907275643376703
21	24.26562571246746	26.222371131363637	30.46040019111475	19.05160296505415
22	23.493038387499034	25.104032640178904	29.639241171351028	21.763687800971038
23	21.49644435368917	26.581999924664313	30.669952360088736	21.251603361557784
24	21.465517070472572	25.68154017870419	32.7591299917329	20.09381275909034
25	21.33625688677244	27.428931680441863	30.33609630280189	20.898715129983803
26	21.236139719949485	27.82265978600699	29.909854899496242	21.031345594547282
27	20.75141403107399	27.623614963254028	30.966537076063272	20.658433929608712
28	19.5307776030959	27.280044567008122	32.14058432740098	21.048593502495
29	21.022226011034697	25.689470251323826	31.698482778856047	21.58982095878543
30	20.95799242281561	26.94321473248891	31.184812324918866	20.913980519776608
31	22.569581430941955	26.774898941137053	29.96893394051256	20.68658568740843
32	23.04637704719781	26.746747183337334	29.949307010778952	20.257568758685906
33	22.046989645307676	26.36570719396363	29.980829049442022	21.606474111286676
34	20.477430022065427	26.206510986124353	31.57259287601926	21.743466115790955
35	21.441132097167177	25.736852435226176	31.254596963971697	21.567418503634947
36	22.203410327730076	28.08038714614529	28.885289517038753	20.83091300908588
37	21.009141391212292	28.41265718890821	30.703258665091226	19.87494275478828
38	20.931228427724328	28.301041416786777	29.67433174269293	21.093398412795967
39	21.166553332712144	27.32286695915418	29.99926646828268	21.511313239850992
40	22.336239044109046	26.586559716420606	29.659859360162088	21.41734187930826
41	20.408041886643577	26.21067427424967	30.403700171884324	22.977583667222433
42	20.874924912124882	26.069122477989094	29.866437751903714	23.189514857982314
43	21.404455511301347	26.027687848551473	30.091850066116983	22.4760065740302
44	20.500823736293363	26.7144321374123	30.088876288884613	22.695867837409722
45	20.958587178262082	27.36112955954394	28.94773883891842	22.732544423275556
46	20.95719941555365	27.87995456068389	29.428895995115074	21.733950028647385
47	20.6168010483556	26.791353841822808	30.415991784444767	22.175853325376828
48	20.691740234611196	25.463264929848595	31.315856774958416	22.52913806058179
49	20.976429841656273	25.610962532389394	31.020065066245845	22.392542559708488
50	20.089252967334048	26.18113475374151	30.790687715722758	22.938924563201688
51	20.037310991675405	26.25904771722947	29.663031389209948	24.040609901885176
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	313.0
1	669.0
2	1025.0
3	3915.5
4	6806.0
5	4755.5
6	2705.0
7	2378.5
8	2052.0
9	2024.0
10	1996.0
11	1983.5
12	1971.0
13	1904.0
14	1837.0
15	1835.0
16	1833.0
17	1842.5
18	1852.0
19	2121.0
20	2390.0
21	2478.5
22	2567.0
23	2868.0
24	3169.0
25	3868.5
26	5564.0
27	6560.0
28	7895.0
29	9230.0
30	10346.5
31	11463.0
32	13514.5
33	15566.0
34	17429.0
35	19292.0
36	20871.0
37	22450.0
38	24305.5
39	26161.0
40	27844.5
41	29528.0
42	31558.0
43	33588.0
44	35052.0
45	36516.0
46	37338.5
47	38161.0
48	37947.5
49	37734.0
50	36023.5
51	34313.0
52	31682.5
53	29052.0
54	27216.0
55	25380.0
56	23615.0
57	21850.0
58	20299.5
59	18749.0
60	17598.0
61	16447.0
62	14545.0
63	12643.0
64	10638.0
65	8633.0
66	7163.0
67	5693.0
68	4611.5
69	3530.0
70	3089.5
71	2649.0
72	2264.0
73	1879.0
74	1452.5
75	806.0
76	586.0
77	453.5
78	321.0
79	230.5
80	140.0
81	118.0
82	96.0
83	70.0
84	44.0
85	28.0
86	12.0
87	13.5
88	15.0
89	12.0
90	9.0
91	7.0
92	5.0
93	4.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.24695474447974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.79009039753984	30.436165192183946
2	7.927887965014807	6.540024722245426
3	3.788978059586787	4.688514196548085
4	2.5066105243901506	4.135602034462287
5	1.800971686756461	3.714229882986654
6	1.4860012664485354	3.6775816192465385
7	1.1769758308773024	3.3982668182079774
8	1.0561237876317007	3.484951205841063
9	0.9207150970756155	3.4179024547473467
>10	5.515274591922478	34.57070982846566
>50	0.02125955492944163	0.6305270329018836
>100	0.00809887806835081	0.5866824378983587
>500	0.0	0.0
>1k	0.0010123597585438512	0.7188425742647861
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2189	0.4339732241097998	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1254	0.24860777662571448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03390106044896106	0.0
2	0.0	0.0	0.0	0.15542942334494428	0.0
3	0.0	0.0	0.0	0.20796615445005937	0.0
4	0.0	0.0	0.0	0.2733892535620895	0.0
5	0.0	0.0	0.0	0.4694602990826888	0.0
6	0.0	0.0	0.0	0.6526449765963732	0.0
7	0.0	0.0	0.0	0.8167974798229215	0.0
8	0.0	0.0	0.0	1.2329280405385312	0.0
9	0.0	0.0	0.0	1.3968822919495885	0.0
10	0.0	0.0	0.0	1.8080565572779232	0.0
11	0.0	0.0	0.0	2.2963507788322572	0.0
12	0.0	0.0	0.0	2.693052661629749	0.0
13	0.0	0.0	0.0	2.8659282447379013	0.0
14	0.0	0.0	0.0	2.9515730290300133	0.0
15	0.0	0.0	0.0	3.0842034935934928	0.0
16	0.0	0.0	0.0	3.366117575221695	0.0
17	0.0	0.0	0.0	3.7120669932534907	0.0
18	0.0	0.0	0.0	4.082401384590679	0.0
19	0.0	0.0	0.0	4.321889577703808	0.0
20	0.0	0.0	0.0	4.5716868652224685	0.0
21	0.0	0.0	0.0	4.895828583550254	0.0
22	0.0	0.0	0.0	5.229288137206116	0.0
23	0.0	0.0	0.0	5.580193850625187	0.0
24	0.0	0.0	0.0	5.876976818415215	0.0
25	0.0	0.0	0.0	6.138272711232353	0.0
26	0.0	0.0	0.0	6.38152768883981	0.0
27	0.0	0.0	0.0	6.640246308055566	0.0
28	0.0	0.0	0.0	6.8983701718248485	0.0
29	0.0	0.0	0.0	7.189602088781128	0.0
30	0.0	0.0	0.0	7.510770029876548	0.0
31	0.0	0.0	0.0	7.83411874094237	0.0
32	0.0	0.0	0.0	8.136254507750655	0.0
33	0.0	0.0	0.0	8.425900410183006	0.0
34	0.0	0.0	0.0	8.727441421544818	0.0
35	0.0	0.0	0.0	9.039688030943143	0.0
36	0.0	0.0	0.0	9.357287439359727	0.0
37	0.0	0.0	0.0	9.659224954352519	0.0
38	0.0	0.0	0.0	10.009139408694136	0.0
39	0.0	0.0	0.0	10.36500141750048	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGT	55	1.8189894E-12	45.000004	28
TATCGCG	35	1.2101191E-7	45.000004	32
CTTCGTA	20	7.029519E-4	45.0	44
CCGGATA	20	7.029519E-4	45.0	21
GAGACCG	25	3.8876824E-5	45.0	6
GTATCCG	20	7.029519E-4	45.0	29
TCCCGAT	20	7.029519E-4	45.0	39
CGCGTAA	25	3.8876824E-5	45.0	31
TCGAATA	20	7.029519E-4	45.0	1
TCGGTAC	20	7.029519E-4	45.0	37
CGAATAT	120	0.0	44.999996	14
GCTACGA	125	0.0	43.2	10
CGTTATT	185	0.0	42.567566	1
AGGCGAT	355	0.0	41.830986	7
CGTTTTA	495	0.0	41.363636	1
ATGATCG	60	3.6379788E-12	41.249996	27
TGTACGG	60	3.6379788E-12	41.249996	2
TAGGCGA	105	0.0	40.714287	6
CTACGAA	135	0.0	40.0	11
TACGAAT	135	0.0	40.0	12
>>END_MODULE