SRR3549753_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549753_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260837
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	1100	0.42171931129402657	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	779	0.2986539486345879	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	549	0.21047627445492778	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	383	0.14683499656873833	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	378	0.14491809060831093	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	359	0.13763384795868686	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	337	0.1291994617328063	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGG	20	7.023246E-4	45.000004	2
CTGTATC	40	6.7793735E-9	45.000004	22
CAACGCG	20	7.023246E-4	45.000004	43
GCGAAGC	20	7.023246E-4	45.000004	2
GCAACTC	20	7.023246E-4	45.000004	9
TTTAACG	20	7.023246E-4	45.000004	1
TTTCGGC	20	7.023246E-4	45.000004	24
GACGTCG	20	7.023246E-4	45.000004	15
TAGCGGC	20	7.023246E-4	45.000004	33
GCTATAC	20	7.023246E-4	45.000004	8
TTGTAAT	20	7.023246E-4	45.000004	37
TATTGCA	20	7.023246E-4	45.000004	15
TACGCCA	20	7.023246E-4	45.000004	26
TGCGCAC	20	7.023246E-4	45.000004	13
TATCTTG	20	7.023246E-4	45.000004	37
GTTCGGT	20	7.023246E-4	45.000004	19
TAACACG	20	7.023246E-4	45.000004	28
AACGCGC	20	7.023246E-4	45.000004	44
GTCGTAA	20	7.023246E-4	45.000004	43
CGCAGAA	20	7.023246E-4	45.000004	10