FastQCFastQC Report
Sat 18 Jun 2016
SRR3549752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460654
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC21680.4706352273072632No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC21530.46737898726593063No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT12550.27243875012482255No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6840.14848454588476384No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC5860.12721044428139122No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC4950.10745592136397383No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC4950.10745592136397383No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG4750.10311426797553044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG302.1626583E-645.0000041
TATTGCG302.1626583E-645.0000041
CGTAGTA253.887153E-545.00000433
TGCGACG302.1626583E-645.0000041
TGCGAAA253.887153E-545.00000426
GCGATAT253.887153E-545.0000049
TAGTCAG302.1626583E-645.00000433
GTTGATC351.2098099E-745.016
CGAAATT207.028881E-445.028
ATATGCG207.028881E-445.01
GCCGATT351.2098099E-745.09
CATCGAA207.028881E-445.041
GCTACGA406.7993824E-945.010
TGGTCAA406.7993824E-945.014
ATCGAGA207.028881E-445.016
CAAAGCG207.028881E-445.01
ATTGGGC1100.040.9090924
CATATTA501.0786607E-940.50000428
AATGGGC1000.040.5000044
AGTACGG501.0786607E-940.5000042