##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549752_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460654
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16185032584109	33.0	31.0	34.0	30.0	34.0
2	32.2710581043473	34.0	31.0	34.0	30.0	34.0
3	32.31513674037347	34.0	31.0	34.0	30.0	34.0
4	35.87663626062077	37.0	35.0	37.0	35.0	37.0
5	35.91672491718296	37.0	35.0	37.0	35.0	37.0
6	35.944513669695695	37.0	35.0	37.0	35.0	37.0
7	36.137033435072745	37.0	35.0	37.0	35.0	37.0
8	35.85853807847104	37.0	35.0	37.0	35.0	37.0
9	37.57774381640016	39.0	37.0	39.0	35.0	39.0
10	37.29962401281656	39.0	37.0	39.0	34.0	39.0
11	37.18326118952619	39.0	37.0	39.0	34.0	39.0
12	36.938893399384355	39.0	35.0	39.0	33.0	39.0
13	36.872494323288194	39.0	35.0	39.0	33.0	39.0
14	37.98731368879897	40.0	36.0	41.0	33.0	41.0
15	38.15266555809784	40.0	37.0	41.0	34.0	41.0
16	38.25616840405163	40.0	37.0	41.0	34.0	41.0
17	38.208049859547515	40.0	36.0	41.0	34.0	41.0
18	38.21735402275895	40.0	36.0	41.0	34.0	41.0
19	38.21944887051887	40.0	36.0	41.0	34.0	41.0
20	38.106557199112565	40.0	36.0	41.0	34.0	41.0
21	38.09443096119865	40.0	36.0	41.0	34.0	41.0
22	38.07281169815089	40.0	36.0	41.0	34.0	41.0
23	38.10897332922323	40.0	36.0	41.0	34.0	41.0
24	38.03142271639886	40.0	36.0	41.0	34.0	41.0
25	37.95964433175442	40.0	35.0	41.0	34.0	41.0
26	37.79727300750672	40.0	35.0	41.0	34.0	41.0
27	37.77078675101052	40.0	35.0	41.0	34.0	41.0
28	37.78996817568066	40.0	35.0	41.0	34.0	41.0
29	37.816866889248764	40.0	36.0	41.0	34.0	41.0
30	37.69743017535938	40.0	36.0	41.0	33.0	41.0
31	37.70419664216527	40.0	35.0	41.0	33.0	41.0
32	37.65113512527841	40.0	35.0	41.0	33.0	41.0
33	37.62045048995559	40.0	35.0	41.0	33.0	41.0
34	37.56064638535647	40.0	35.0	41.0	33.0	41.0
35	37.52436535881594	40.0	35.0	41.0	33.0	41.0
36	37.44882710233711	40.0	35.0	41.0	33.0	41.0
37	37.4080720019798	40.0	35.0	41.0	33.0	41.0
38	37.33936750793437	39.0	35.0	41.0	33.0	41.0
39	37.31035875081949	39.0	35.0	41.0	33.0	41.0
40	37.23696310028785	39.0	35.0	41.0	33.0	41.0
41	37.13967316033292	39.0	35.0	41.0	33.0	41.0
42	37.1124054062268	39.0	35.0	41.0	33.0	41.0
43	37.07181745952494	39.0	35.0	41.0	33.0	41.0
44	37.005613757831256	39.0	35.0	41.0	33.0	41.0
45	36.895524623687194	39.0	35.0	41.0	32.0	41.0
46	36.82867618646533	39.0	35.0	41.0	32.0	41.0
47	36.778050337129386	39.0	35.0	41.0	32.0	41.0
48	36.751294898123106	39.0	35.0	41.0	32.0	41.0
49	36.73026609993618	39.0	35.0	41.0	32.0	41.0
50	36.6090992371715	38.0	35.0	41.0	32.0	41.0
51	35.65809262483339	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	12.0
10	7.0
11	3.0
12	7.0
13	13.0
14	12.0
15	18.0
16	33.0
17	39.0
18	79.0
19	129.0
20	243.0
21	403.0
22	617.0
23	931.0
24	1258.0
25	1698.0
26	2334.0
27	2931.0
28	3678.0
29	4574.0
30	5878.0
31	7531.0
32	10299.0
33	15288.0
34	33468.0
35	46145.0
36	31195.0
37	46487.0
38	85905.0
39	159285.0
40	144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.25640936581469	20.206271952484943	22.61523833506276	10.922080346637607
2	34.76470409461331	23.035727465733498	23.56497501378475	18.634593425868438
3	33.752447607097736	23.900367738042	24.964289900880054	17.38289475398021
4	31.035657999279287	25.70519305161792	23.839584590603792	19.419564358499002
5	25.824588519800113	29.67237883530806	23.204400699874526	21.298631945017302
6	28.11155444216266	33.85794978443691	23.110403904014724	14.920091869385699
7	81.6252545294299	4.775601644618304	8.79901183968879	4.8001319862630085
8	80.57479149209603	4.943189465412218	8.762107785887022	5.71991125660474
9	73.72279411445467	7.496732905825196	10.966148128530307	7.81432485118983
10	43.46537748505386	24.784979615937342	17.497297320765696	14.252345578243105
11	33.83602443482527	22.956926456733253	23.84436040933108	19.362688699110393
12	31.400356883908533	20.954772996652586	28.157575968080163	19.487294151358718
13	25.510035731807385	24.985346919814003	30.16016359349968	19.34445375487893
14	21.376130458001015	27.1346824297629	29.673464248655172	21.81572286358091
15	20.671697195726075	25.07217998758287	34.14884056146262	20.107282255228437
16	23.773591459099457	23.40802424379252	31.486755786338556	21.33162851076947
17	22.62652663387271	24.266369118687777	27.437078588267983	25.670025659171525
18	23.549779226925196	24.211881368662812	30.28455196307858	21.953787441333407
19	23.73408241326462	26.34971149713234	27.496342157020237	22.419863932582807
20	25.32052256140183	27.120137890911618	27.8827493085917	19.676590239094853
21	24.45675061977102	26.98728329722525	28.552666426428512	20.003299656575216
22	22.007189778011263	24.878759329127718	27.86798768707099	25.24606320579003
23	21.881281829746406	25.713442193055958	28.77126867453664	23.634007302661
24	24.00934323809193	23.37112018999075	29.58489451953093	23.03464205238639
25	21.015339061421372	25.370885740707777	28.61757414458574	24.996201053285112
26	20.38905556013841	26.256148866611383	29.833888341358154	23.52090723189205
27	22.420298097921652	25.715830102419602	28.643841147585825	23.220030652072925
28	18.645230476670125	27.292935695771664	29.929187633234488	24.132646194323723
29	22.633039113955377	23.511355594437475	30.49924672313711	23.356358568470046
30	25.81872728772571	25.27992810221989	27.7427309868144	21.15861362324
31	25.828061842510863	26.96079921155575	25.28535516895544	21.92578377697795
32	27.26818827145754	26.71115414172025	25.101051982616017	20.919605604206193
33	27.156824862043965	25.65157363227064	25.047649645938165	22.14395185974723
34	23.992844955215844	25.30576093988113	28.126316063683372	22.57507804121966
35	23.558462533702084	26.120689280891952	28.012998910245003	22.30784927516097
36	25.61228166910523	28.514242793940785	26.02235083164371	19.851124705310276
37	24.521224172589406	28.865265470396434	27.162034846110096	19.45147551090406
38	24.190173101720596	29.274032136918382	24.227077155522366	22.308717605838655
39	26.585897441463658	25.62921411732016	26.03776370117268	21.747124740043503
40	26.46715322128973	23.687409639338853	27.600107673004032	22.245329466367384
41	21.585615233993412	26.39269386567793	26.406370073851527	25.615320826477138
42	22.366244513235532	26.322141998115722	27.466167665970552	23.845445822678194
43	23.694573367429786	24.845545680706127	27.659371241756286	23.800509710107804
44	23.427344601371093	24.392711232291482	26.801460532199872	25.37848363413755
45	24.515145857845585	23.793997230025138	26.264180925380003	25.426675986749274
46	25.811346476965358	25.120372340194592	26.70399041362932	22.364290769210733
47	21.73648768924182	25.1370877057401	29.648065576332776	23.478359028685304
48	22.553803939616284	24.00825782474482	29.44682994177843	23.99110829386047
49	24.373825040051752	22.931961949749706	30.072896360391965	22.62131664980658
50	23.093471455799797	22.672982325129055	29.423819178819677	24.80972704025147
51	21.574109852514034	23.758395672239903	26.64190477017458	28.025589705071486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	428.5
2	621.0
3	953.5
4	1286.0
5	872.5
6	459.0
7	485.0
8	511.0
9	518.5
10	526.0
11	567.5
12	609.0
13	659.5
14	710.0
15	741.5
16	773.0
17	765.0
18	757.0
19	772.5
20	788.0
21	921.5
22	1055.0
23	1406.5
24	1758.0
25	1881.5
26	2258.5
27	2512.0
28	3179.0
29	3846.0
30	4430.5
31	5015.0
32	5881.0
33	6747.0
34	7957.0
35	9167.0
36	10339.5
37	11512.0
38	12598.0
39	13684.0
40	15831.0
41	17978.0
42	21130.0
43	24282.0
44	27395.0
45	30508.0
46	35965.0
47	41422.0
48	45740.0
49	50058.0
50	51749.0
51	53440.0
52	46468.0
53	39496.0
54	34912.5
55	30329.0
56	26934.0
57	23539.0
58	21516.0
59	19493.0
60	18310.5
61	17128.0
62	16139.5
63	15151.0
64	13510.5
65	11870.0
66	9709.0
67	7548.0
68	6158.5
69	4769.0
70	4292.0
71	3815.0
72	2888.0
73	1961.0
74	1749.5
75	1228.5
76	919.0
77	668.5
78	418.0
79	317.0
80	216.0
81	186.0
82	156.0
83	91.5
84	27.0
85	16.5
86	6.0
87	5.5
88	5.0
89	2.5
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	460654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.66853889098046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.82708326079388	21.108260797364075
2	12.8518587779906	7.882954615083866
3	4.752582480274293	4.372642818866536
4	2.4171076675370426	2.9651664202218746
5	1.3117824291791942	2.0115225222893467
6	0.9530504710905422	1.7537199262624525
7	0.726472783568647	1.559590117127972
8	0.535692363073827	1.314312165642471
9	0.4647558814944136	1.2828045443780998
>10	6.941574035040405	48.052002081156225
>50	0.16656814767222913	3.3219958276363717
>100	0.04789727851519036	2.8740788875716206
>500	0.00142976950791613	0.27844078016751533
>1k	0.002144654261874195	1.2225084962315476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2168	0.4706352273072632	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2153	0.46737898726593063	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	1255	0.27243875012482255	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	684	0.14848454588476384	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	586	0.12721044428139122	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	495	0.10745592136397383	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	495	0.10745592136397383	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	475	0.10311426797553044	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.283292883595931	0.0
2	0.0	0.0	0.0	1.4837600455005275	0.0
3	0.0	0.0	0.0	2.018868825626175	0.0
4	0.0	0.0	0.0	2.6994229942646757	0.0
5	0.0	0.0	0.0	4.72024556391565	0.0
6	0.0	0.0	0.0	5.662167266538443	0.0
7	0.0	0.0	0.0	6.60756229187199	0.0
8	0.0	0.0	0.0	8.078297377207187	0.0
9	0.0	0.0	0.0	8.577153351539334	0.0
10	0.0	0.0	0.0	9.837969495543293	0.0
11	0.0	0.0	0.0	11.760670698615447	0.0
12	0.0	0.0	0.0	13.273302739149123	0.0
13	0.0	0.0	0.0	13.862030938622047	0.0
14	0.0	0.0	0.0	14.107985603077363	0.0
15	0.0	0.0	0.0	14.623991108293861	0.0
16	0.0	0.0	0.0	15.674019980288893	0.0
17	0.0	0.0	0.0	16.811099002722216	0.0
18	0.0	0.0	0.0	17.97596460684158	0.0
19	0.0	0.0	0.0	18.753554728711787	0.0
20	0.0	0.0	0.0	19.516166146391868	0.0
21	0.0	0.0	0.0	20.403165933650854	0.0
22	0.0	0.0	0.0	21.358980927116665	0.0
23	0.0	0.0	0.0	22.265952319962487	0.0
24	0.0	0.0	0.0	23.013367950783017	0.0
25	0.0	0.0	0.0	23.70607874890916	0.0
26	0.0	0.0	0.0	24.333881828878074	0.0
27	0.0	0.0	0.0	24.933247079152682	0.0
28	0.0	0.0	0.0	25.557793919080265	0.0
29	0.0	0.0	0.0	26.203180695272373	0.0
30	0.0	0.0	0.0	26.93431512588624	0.0
31	0.0	0.0	0.0	27.568413603268397	0.0
32	0.0	0.0	0.0	28.224437430262192	0.0
33	0.0	0.0	0.0	28.85571383294186	0.0
34	0.0	0.0	0.0	29.47505068880331	0.0
35	0.0	0.0	0.0	30.146270302656657	0.0
36	0.0	0.0	0.0	30.734564336790736	0.0
37	0.0	0.0	0.0	31.29420345856109	0.0
38	0.0	0.0	0.0	31.95261519491853	0.0
39	0.0	0.0	0.0	32.621663982077656	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	30	2.1626583E-6	45.000004	1
TATTGCG	30	2.1626583E-6	45.000004	1
CGTAGTA	25	3.887153E-5	45.000004	33
TGCGACG	30	2.1626583E-6	45.000004	1
TGCGAAA	25	3.887153E-5	45.000004	26
GCGATAT	25	3.887153E-5	45.000004	9
TAGTCAG	30	2.1626583E-6	45.000004	33
GTTGATC	35	1.2098099E-7	45.0	16
CGAAATT	20	7.028881E-4	45.0	28
ATATGCG	20	7.028881E-4	45.0	1
GCCGATT	35	1.2098099E-7	45.0	9
CATCGAA	20	7.028881E-4	45.0	41
GCTACGA	40	6.7993824E-9	45.0	10
TGGTCAA	40	6.7993824E-9	45.0	14
ATCGAGA	20	7.028881E-4	45.0	16
CAAAGCG	20	7.028881E-4	45.0	1
ATTGGGC	110	0.0	40.909092	4
CATATTA	50	1.0786607E-9	40.500004	28
AATGGGC	100	0.0	40.500004	4
AGTACGG	50	1.0786607E-9	40.500004	2
>>END_MODULE