FastQCFastQC Report
Sat 18 Jun 2016
SRR3549748_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549748_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359647
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175774.887292261578715No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43191.200899771164503No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT4940.13735690830175143No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA4730.13151784944681869No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC4130.11483482414701082Illumina Single End Adapter 1 (95% over 21bp)
TTGTGTGTGTGCTATGAGACTGTACATTTTTTTAAATGGCAATATGCAATA3880.1078835636054242No Hit
GGGCGTGGGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC3830.10649331149710688No Hit
GCTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCA3760.10454695854546264No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC3700.10287865601548184Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTTA551.8189894E-1245.0000047
AACCCGA900.045.00000424
CTTTTCG453.8380676E-1045.0000041
CGGGAAT453.8380676E-1045.0000046
TCGTAGG351.2088094E-745.0000042
TATAGGG351.2088094E-745.0000043
TCACGAC253.8854418E-545.025
TCGCCGG253.8854418E-545.026
ATTTAGG207.026815E-445.02
TTTAGCG207.026815E-445.01
CGTGGCC207.026815E-445.041
TCGTTTA253.8854418E-545.038
CTCACGT253.8854418E-545.045
ACGTTTG207.026815E-445.015
CAACGCG207.026815E-445.032
GAATACC207.026815E-445.028
TAACGCC253.8854418E-545.012
TGACGAC207.026815E-445.015
TGATTTG207.026815E-445.01
ATGAGAC800.045.014