##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549748_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359647
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.191340397667716	33.0	31.0	34.0	30.0	34.0
2	32.439656107238484	34.0	31.0	34.0	31.0	34.0
3	32.31686069951925	33.0	31.0	34.0	30.0	34.0
4	35.869463668541655	37.0	35.0	37.0	35.0	37.0
5	35.968293910417714	37.0	35.0	37.0	35.0	37.0
6	28.929422461469162	37.0	33.0	37.0	0.0	37.0
7	32.525284515093965	37.0	35.0	37.0	17.0	37.0
8	34.99216731962174	37.0	35.0	37.0	32.0	37.0
9	37.34893103515392	39.0	37.0	39.0	35.0	39.0
10	37.53578091851176	39.0	37.0	39.0	35.0	39.0
11	37.51606158260739	39.0	37.0	39.0	35.0	39.0
12	36.999838730755435	39.0	35.0	39.0	34.0	39.0
13	36.65029876517808	39.0	35.0	39.0	33.0	39.0
14	37.66642568963456	40.0	35.0	41.0	33.0	41.0
15	38.00806902323667	40.0	35.0	41.0	33.0	41.0
16	38.161419391792506	40.0	35.0	41.0	34.0	41.0
17	38.15662580252303	40.0	35.0	41.0	34.0	41.0
18	37.886861283425134	39.0	36.0	41.0	34.0	41.0
19	37.49379530484058	38.0	36.0	41.0	34.0	41.0
20	37.13088667498964	38.0	35.0	41.0	34.0	41.0
21	36.88491493047349	37.0	35.0	40.0	33.0	41.0
22	36.851946491976854	37.0	35.0	40.0	33.0	41.0
23	36.770052301284316	35.0	35.0	40.0	34.0	41.0
24	36.63485028375045	35.0	35.0	40.0	33.0	41.0
25	36.442450513976205	35.0	35.0	40.0	33.0	41.0
26	36.46943530739864	35.0	35.0	40.0	33.0	41.0
27	36.44639604945961	35.0	35.0	40.0	33.0	41.0
28	36.32872789151585	35.0	35.0	40.0	33.0	41.0
29	36.21498580552598	36.0	35.0	40.0	33.0	41.0
30	35.787277524906365	36.0	35.0	40.0	32.0	41.0
31	35.376761101858214	35.0	35.0	40.0	31.0	41.0
32	34.67022107789026	35.0	35.0	40.0	24.0	41.0
33	33.91219723784711	35.0	34.0	40.0	18.0	41.0
34	33.24464544400482	35.0	34.0	40.0	15.0	41.0
35	32.899409698954805	35.0	33.0	40.0	10.0	41.0
36	32.654522351083145	35.0	33.0	40.0	10.0	41.0
37	32.59309545192925	35.0	33.0	40.0	10.0	41.0
38	32.4731389390152	35.0	33.0	40.0	10.0	41.0
39	32.40796113967307	35.0	33.0	40.0	10.0	41.0
40	32.28380328488768	35.0	32.0	40.0	10.0	41.0
41	32.18382469477015	35.0	32.0	40.0	10.0	41.0
42	32.11425369876574	35.0	32.0	40.0	10.0	41.0
43	32.00060336941501	35.0	32.0	40.0	10.0	41.0
44	31.867267070210513	35.0	31.0	40.0	9.0	41.0
45	31.797545926978398	35.0	31.0	40.0	8.0	41.0
46	31.728508787783575	35.0	31.0	40.0	8.0	41.0
47	31.713872213587212	35.0	31.0	40.0	8.0	41.0
48	31.682313490728408	35.0	31.0	40.0	8.0	41.0
49	31.63054050221467	35.0	31.0	40.0	8.0	41.0
50	31.498210745536596	35.0	31.0	39.0	8.0	41.0
51	30.740150758938626	35.0	29.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	20.0
10	27.0
11	20.0
12	14.0
13	19.0
14	23.0
15	39.0
16	60.0
17	70.0
18	179.0
19	301.0
20	573.0
21	909.0
22	1398.0
23	2462.0
24	4817.0
25	9017.0
26	13504.0
27	15008.0
28	12809.0
29	10125.0
30	8619.0
31	8859.0
32	10918.0
33	18026.0
34	31132.0
35	47175.0
36	26286.0
37	32595.0
38	46762.0
39	57829.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.603666928960898	3.3852638837526796	38.115429852049374	38.89563933523705
2	42.187200226889146	3.580455279760432	37.770925379608336	16.46141911374209
3	17.739338851707366	3.445878875675315	61.29315690107244	17.52162537154488
4	15.750166135126944	3.884086340216935	60.765695251176844	19.600052273479275
5	14.709701457262259	5.142820599087439	59.19220791498331	20.955270028666998
6	13.92254071353299	24.817668435994182	49.68371764535781	11.576073205115016
7	63.304573651385944	2.456853525818372	30.8305088044666	3.4080640183290836
8	64.44819503568777	2.352028516851246	29.60236009197908	3.5974163554819025
9	61.72635945802411	3.7456172302285298	30.66757125737181	3.8604520543755405
10	40.71770374839773	14.066848882376329	35.52010721624259	9.695340152983341
11	33.656057189410724	16.31599874321209	37.865462522973914	12.162481544403263
12	30.83996251880316	14.995815341153964	41.70228029150807	12.461941848534813
13	18.72252514270938	25.26532961487236	42.602329506432696	13.409815735985564
14	13.001637716983597	28.04221917602538	44.191943767082726	14.764199339908298
15	11.913070316171135	19.103176169966662	55.850597947431794	13.133155566430416
16	14.509505153664565	14.275108648202265	56.50457253918425	14.710813658948913
17	13.715949250237038	14.915736819714887	45.41675587451029	25.951558055537795
18	16.85430435955256	16.852079956179253	49.0628310537833	17.23078463048489
19	19.644540340945426	18.9377361690769	44.135221481063375	17.282502008914296
20	21.86310465539821	18.051311424813775	45.05890498182941	15.026678937958609
21	18.08606772752171	19.510241987281972	45.661440245574134	16.742250039622185
22	18.39108904008653	17.09259357091815	44.9835533175586	19.532764071436716
23	15.964264959807812	20.093591771931923	44.07822114462236	19.8639221236379
24	16.47532163482526	18.126663089084573	49.04809438143513	16.34992089465504
25	15.826908051506061	17.014183352009056	48.605994210990225	18.552914385494663
26	13.752651905896615	22.581308894554937	43.61304278917939	20.052996410369055
27	14.249806059830888	23.101819283908945	46.39604945960901	16.25232519665116
28	12.621542790569643	21.58088347740979	50.280413850247605	15.51715988177296
29	14.352128615003046	19.273621078446364	48.83093700211597	17.543313304434626
30	14.957444382964407	21.377906669595465	47.48267050746983	16.181978439970305
31	20.637180346283994	21.497468350910754	40.8450508415196	17.02030046128565
32	21.278086568218278	21.868387613409816	41.059983817465465	15.793542000906443
33	20.69056602724338	22.512630440404063	38.71879926705909	18.07800426529347
34	16.25482765044613	24.427841744822008	38.609247400923685	20.70808320380818
35	17.916178919885333	22.20760912783924	37.29379085603383	22.582421096241593
36	22.980867350485337	22.10890122814871	35.55625377105884	19.353977650307105
37	18.6888810416881	25.397959666005832	38.470500240513616	17.442659051792454
38	18.793149949811898	26.339994494601648	34.620614102161284	20.246241453425164
39	17.962057239459803	25.126026353618965	36.800807458424515	20.11110894849672
40	19.307265179467645	23.332879184311285	35.45615561926	21.903700016961075
41	16.250100793277852	22.467864322516245	37.78288154773987	23.499153336466037
42	19.04700998479064	22.83683723206366	35.25846176945727	22.857691013688424
43	19.05423929575389	22.798466273874105	35.35883797167779	22.78845645869422
44	17.596142884550684	24.82600994864409	35.9994661431904	21.578381023614824
45	17.942871760365026	28.445392287437404	32.36034222445898	21.251393727738588
46	19.809702291413526	25.99159731625733	35.35438916493116	18.844311227397974
47	17.67510920430311	25.453013649495198	36.080100765472814	20.791776380728884
48	18.887687093177476	23.425469974725218	37.706695732204075	19.980147199893228
49	19.23080131351019	21.504975712295668	37.751739900513556	21.51248307368058
50	18.734203260419243	23.323981570818052	36.54833767555408	21.393477493208618
51	16.3791161889297	26.13312498088403	33.48422202882271	24.00353680136356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	294.5
2	534.0
3	15562.5
4	30591.0
5	18864.5
6	7138.0
7	7294.5
8	7451.0
9	7756.0
10	8061.0
11	7935.5
12	7810.0
13	7252.0
14	6694.0
15	6156.5
16	5619.0
17	5194.5
18	4770.0
19	4364.5
20	3959.0
21	3765.5
22	3572.0
23	3277.0
24	2982.0
25	2906.0
26	2860.5
27	2891.0
28	3079.5
29	3268.0
30	3814.0
31	4360.0
32	4422.0
33	4484.0
34	5195.0
35	5906.0
36	6034.5
37	6163.0
38	7372.0
39	8581.0
40	12052.5
41	15524.0
42	18906.5
43	22289.0
44	23655.5
45	25022.0
46	24126.5
47	23231.0
48	24433.5
49	25636.0
50	24527.5
51	23419.0
52	21770.5
53	20122.0
54	17526.5
55	14931.0
56	14301.5
57	13672.0
58	13073.5
59	12475.0
60	11607.0
61	10739.0
62	9442.5
63	8146.0
64	6792.0
65	5438.0
66	4662.5
67	3887.0
68	3148.5
69	2410.0
70	2065.5
71	1721.0
72	1550.0
73	1379.0
74	1144.5
75	716.0
76	522.0
77	408.0
78	294.0
79	189.0
80	84.0
81	56.0
82	28.0
83	32.5
84	37.0
85	21.0
86	5.0
87	3.5
88	2.0
89	3.0
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	359647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.782066231098135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06689523944435	29.117465401617874
2	8.253769050394485	6.236888978363848
3	3.3542166929375545	3.80187711738065
4	1.9791294983098469	2.991024071402506
5	1.3011822006496212	2.4580676041835003
6	0.9896013984735876	2.2433511347709856
7	0.7979391583798328	2.1103453091205484
8	0.610626373292556	1.845658086255568
9	0.49641102855178687	1.6879890922732042
>10	4.959480490770004	34.23942752677589
>50	0.1340397456407058	3.423410166407776
>100	0.05523615891787327	3.7517547975027266
>500	0.0	0.0
>1k	7.36482118904977E-4	1.2017970014399078
>5k	0.0	0.0
>10k+	7.36482118904977E-4	4.890943712505038
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17577	4.887292261578715	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4319	1.200899771164503	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	494	0.13735690830175143	No Hit
TTTTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	473	0.13151784944681869	No Hit
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	413	0.11483482414701082	Illumina Single End Adapter 1 (95% over 21bp)
TTGTGTGTGTGCTATGAGACTGTACATTTTTTTAAATGGCAATATGCAATA	388	0.1078835636054242	No Hit
GGGCGTGGGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC	383	0.10649331149710688	No Hit
GCTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCA	376	0.10454695854546264	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	370	0.10287865601548184	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7805042166346446E-4	0.0	0.0	0.074239462584145	0.0
2	2.7805042166346446E-4	0.0	0.0	0.39594380044877336	0.0
3	2.7805042166346446E-4	0.0	0.0	0.5274616498955921	0.0
4	2.7805042166346446E-4	0.0	0.0	0.6831698860271321	0.0
5	2.7805042166346446E-4	0.0	0.0	0.9845765431103276	0.0
6	2.7805042166346446E-4	0.0	0.0	1.1653093171915796	0.0
7	2.7805042166346446E-4	0.0	0.0	1.4024863268705148	0.0
8	2.7805042166346446E-4	0.0	0.0	1.6936051183521619	0.0
9	2.7805042166346446E-4	0.0	0.0	1.8309620266539135	0.0
10	2.7805042166346446E-4	0.0	0.0	2.2146716085494944	0.0
11	2.7805042166346446E-4	0.0	0.0	3.0816328232961765	0.0
12	2.7805042166346446E-4	0.0	0.0	3.782319885888107	0.0
13	2.7805042166346446E-4	0.0	0.0	3.994750408038994	0.0
14	2.7805042166346446E-4	0.0	0.0	4.070102072309792	0.0
15	2.7805042166346446E-4	0.0	0.0	4.210795585671506	0.0
16	2.7805042166346446E-4	0.0	0.0	4.529997469741163	0.0
17	2.7805042166346446E-4	0.0	0.0	4.934004732418177	0.0
18	5.561008433269289E-4	0.0	0.0	5.448119962073923	0.0
19	5.561008433269289E-4	0.0	0.0	5.783170720178397	0.0
20	5.561008433269289E-4	0.0	0.0	6.153255831412468	0.0
21	5.561008433269289E-4	0.0	0.0	6.740776372387368	0.0
22	5.561008433269289E-4	0.0	0.0	7.248496442344855	0.0
23	5.561008433269289E-4	0.0	0.0	7.742313991219167	0.0
24	5.561008433269289E-4	0.0	0.0	8.119628413416489	0.0
25	5.561008433269289E-4	0.0	0.0	8.448284011822704	0.0
26	5.561008433269289E-4	0.0	0.0	8.758866332820794	0.0
27	5.561008433269289E-4	0.0	0.0	9.027741090569364	0.0
28	5.561008433269289E-4	0.0	0.0	9.348889327590665	0.0
29	5.561008433269289E-4	0.0	0.0	9.681437631900168	0.0
30	8.341512649903933E-4	0.0	0.0	10.121869499815096	0.0
31	0.0011122016866538578	0.0	0.0	10.518925501950523	0.0
32	0.0011122016866538578	0.0	0.0	10.890678915714576	2.7805042166346446E-4
33	0.0011122016866538578	0.0	0.0	11.215719858639165	2.7805042166346446E-4
34	0.0011122016866538578	0.0	0.0	11.55633162517691	2.7805042166346446E-4
35	0.0011122016866538578	0.0	0.0	12.010387963753347	2.7805042166346446E-4
36	0.0011122016866538578	0.0	0.0	12.40160490703384	2.7805042166346446E-4
37	0.0011122016866538578	0.0	0.0	12.812007329409115	2.7805042166346446E-4
38	0.0011122016866538578	0.0	0.0	13.194882760039706	2.7805042166346446E-4
39	0.0011122016866538578	0.0	0.0	13.618631602654826	2.7805042166346446E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCTTA	55	1.8189894E-12	45.000004	7
AACCCGA	90	0.0	45.000004	24
CTTTTCG	45	3.8380676E-10	45.000004	1
CGGGAAT	45	3.8380676E-10	45.000004	6
TCGTAGG	35	1.2088094E-7	45.000004	2
TATAGGG	35	1.2088094E-7	45.000004	3
TCACGAC	25	3.8854418E-5	45.0	25
TCGCCGG	25	3.8854418E-5	45.0	26
ATTTAGG	20	7.026815E-4	45.0	2
TTTAGCG	20	7.026815E-4	45.0	1
CGTGGCC	20	7.026815E-4	45.0	41
TCGTTTA	25	3.8854418E-5	45.0	38
CTCACGT	25	3.8854418E-5	45.0	45
ACGTTTG	20	7.026815E-4	45.0	15
CAACGCG	20	7.026815E-4	45.0	32
GAATACC	20	7.026815E-4	45.0	28
TAACGCC	25	3.8854418E-5	45.0	12
TGACGAC	20	7.026815E-4	45.0	15
TGATTTG	20	7.026815E-4	45.0	1
ATGAGAC	80	0.0	45.0	14
>>END_MODULE