SRR3549746_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549746_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489451
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10888	2.2245332014849293	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	3026	0.6182437057029202	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2618	0.5348850038103916	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	2027	0.4141374723925378	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	1462	0.298702015114894	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1135	0.23189246727455862	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1008	0.20594502820507057	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	972	0.19858984862631807	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	849	0.17345965173224695	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	813	0.16610447215349441	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	588	0.12013459978629118	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	492	0.10052078757628445	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAGCG	20	7.029312E-4	45.000004	21
TCCGACC	20	7.029312E-4	45.000004	34
GGGTACG	20	7.029312E-4	45.000004	7
CCAATAG	20	7.029312E-4	45.000004	21
CGATTGA	20	7.029312E-4	45.000004	10
CACGACC	20	7.029312E-4	45.000004	27
AGGCGAA	20	7.029312E-4	45.000004	22
GCGTTGC	20	7.029312E-4	45.000004	1
CGTTCAT	20	7.029312E-4	45.000004	17
CATTACG	20	7.029312E-4	45.000004	22
AAACGCC	20	7.029312E-4	45.000004	17
ATATACG	20	7.029312E-4	45.000004	18
TGTAGCG	20	7.029312E-4	45.000004	1
CGATGAC	20	7.029312E-4	45.000004	12
CGGTAAA	20	7.029312E-4	45.000004	22
GCTGTCG	20	7.029312E-4	45.000004	1
GCCGTAA	20	7.029312E-4	45.000004	26
ACGACCA	20	7.029312E-4	45.000004	28
CGGGTCA	25	3.887513E-5	45.0	6
CGGGTAT	50	2.1827873E-11	45.0	6