##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549746_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489451
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09450588516522	33.0	31.0	34.0	30.0	34.0
2	32.32900331187392	34.0	31.0	34.0	30.0	34.0
3	32.33817481218753	34.0	31.0	34.0	30.0	34.0
4	35.800932064701065	37.0	35.0	37.0	35.0	37.0
5	35.872203754819175	37.0	35.0	37.0	35.0	37.0
6	28.933944358066487	37.0	33.0	37.0	0.0	37.0
7	32.466876153077635	37.0	35.0	37.0	17.0	37.0
8	34.878185967543224	37.0	33.0	37.0	32.0	37.0
9	37.35647490760056	39.0	37.0	39.0	35.0	39.0
10	37.19363940414873	39.0	37.0	39.0	34.0	39.0
11	37.256755017356184	39.0	37.0	39.0	34.0	39.0
12	37.164396436006875	39.0	37.0	39.0	34.0	39.0
13	37.08225746806115	39.0	37.0	39.0	33.0	39.0
14	38.24259016735077	40.0	37.0	41.0	33.0	41.0
15	38.36899505772794	40.0	37.0	41.0	34.0	41.0
16	38.4497365415537	40.0	37.0	41.0	34.0	41.0
17	38.41720417365579	40.0	37.0	41.0	34.0	41.0
18	38.293530915249946	40.0	37.0	41.0	34.0	41.0
19	38.15436274519819	40.0	37.0	41.0	34.0	41.0
20	37.98612935717774	40.0	35.0	41.0	34.0	41.0
21	37.8673758966679	39.0	35.0	41.0	34.0	41.0
22	37.8405478791544	39.0	35.0	41.0	34.0	41.0
23	37.763627002498716	39.0	35.0	41.0	34.0	41.0
24	37.62729466279566	39.0	35.0	41.0	34.0	41.0
25	37.44458587274313	39.0	35.0	41.0	33.0	41.0
26	37.44982030887668	39.0	35.0	41.0	33.0	41.0
27	37.45398211465499	39.0	35.0	41.0	33.0	41.0
28	37.389681500293186	39.0	35.0	41.0	33.0	41.0
29	37.359394505272235	39.0	35.0	41.0	33.0	41.0
30	37.11468359447626	39.0	35.0	41.0	33.0	41.0
31	37.00717130008928	39.0	35.0	41.0	33.0	41.0
32	36.712189779977976	39.0	35.0	41.0	32.0	41.0
33	36.366964210922035	39.0	35.0	41.0	31.0	41.0
34	36.05374388856086	39.0	35.0	41.0	29.0	41.0
35	35.85394043530405	39.0	35.0	41.0	27.0	41.0
36	35.67519118359141	39.0	35.0	41.0	26.0	41.0
37	35.64272010885666	39.0	35.0	41.0	25.0	41.0
38	35.54677179125183	39.0	35.0	41.0	25.0	41.0
39	35.508030425926194	39.0	35.0	41.0	25.0	41.0
40	35.34164400522218	39.0	35.0	41.0	23.0	41.0
41	35.277523184138964	39.0	35.0	41.0	23.0	41.0
42	35.28783065107641	39.0	35.0	41.0	23.0	41.0
43	35.230817793813884	39.0	35.0	41.0	23.0	41.0
44	35.12877080647501	39.0	35.0	41.0	23.0	41.0
45	35.07105716404706	38.0	35.0	41.0	23.0	41.0
46	34.95488618881154	38.0	35.0	40.0	23.0	41.0
47	34.92030458615878	38.0	35.0	40.0	23.0	41.0
48	34.89247953319127	38.0	35.0	40.0	23.0	41.0
49	34.86708373259019	38.0	35.0	40.0	23.0	41.0
50	34.75718713415643	38.0	34.0	40.0	22.0	41.0
51	33.838504773715854	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	29.0
10	26.0
11	23.0
12	18.0
13	15.0
14	25.0
15	28.0
16	39.0
17	89.0
18	145.0
19	232.0
20	487.0
21	802.0
22	1284.0
23	2091.0
24	3427.0
25	5937.0
26	8854.0
27	10156.0
28	9300.0
29	8604.0
30	8813.0
31	10198.0
32	13031.0
33	19380.0
34	31123.0
35	45920.0
36	40231.0
37	57843.0
38	90788.0
39	120443.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.61411254650619	4.000604759209809	49.15405219317153	24.23123050111247
2	25.833638096561252	6.61925299978956	48.768313886374735	18.778795017274458
3	17.942551961279065	6.246181946711724	57.21103849006336	18.600227601945853
4	15.982805224629226	4.6999597508228605	57.86993999399327	21.447295030554642
5	16.851125036009734	5.134323967056968	56.15332280452997	21.861228192403324
6	15.547828076763556	26.00198998469714	46.517424624732605	11.932757313806693
7	73.0183409575218	2.661349144245287	20.070650586064794	4.249659312168124
8	73.95326600619879	5.123904129320402	16.852350899272857	4.070478965207958
9	68.35250106752258	5.611593397500465	19.343305049943712	6.692600485033232
10	34.88684260528633	25.483449824395088	26.36443688949456	13.265270680824026
11	27.134279018737317	22.115390508957997	34.47883444920942	16.271496023095263
12	25.82485274317552	19.941322011805063	38.528065117856535	15.705760127162883
13	19.746001131880416	25.007406257214715	39.74350854324539	15.50308406765948
14	15.821604205528233	27.5696647876907	39.128329495700285	17.48040151108078
15	14.01059554480428	23.114673378949067	46.66922735881631	16.205503717430343
16	14.903636931991148	21.587247753094793	44.3686906350176	19.140424679896455
17	16.082917391117803	21.68449957196941	38.703567875027325	23.529015161885457
18	17.01641226598781	22.619220310102545	42.29882051523033	18.065546908679316
19	19.46139654429146	23.907398289103508	37.431939050078554	19.199266116526477
20	21.65548747474211	22.78491616116833	39.07030530124568	16.48929106284388
21	19.362714551609862	24.961027763759805	38.18073719330434	17.495520491325998
22	18.451693836563823	23.18761224310503	36.506412286418865	21.854281633912283
23	17.44934630841494	25.188629709613426	36.07776876541268	21.28425521655896
24	16.867469879518072	22.351164876565786	41.71285787545638	19.068507368459763
25	15.26342779971846	27.028854778108535	37.776610937560655	19.93110648461235
26	15.190488935562499	26.481506831123035	37.545535712461515	20.782468520852955
27	15.391939131802776	27.85590385963048	38.252654504741024	18.499502503825717
28	13.739475453109709	26.140103912342603	41.30178506122165	18.81863557332603
29	15.81567919975646	23.28404681980423	38.412833971122744	22.48744000931656
30	15.701673916285797	26.25901264886577	40.36542983873769	17.673883596110745
31	18.34259200614566	25.75988199022987	37.80664458750723	18.09088141611724
32	19.876351258859415	23.578866934585893	37.060502481351556	19.484279325203136
33	18.10130125385381	25.0525588874065	36.375244917264446	20.470894941475244
34	16.76163701780158	24.569568761735088	36.65208570418694	22.0167085162764
35	17.377224686434392	25.142455526702367	35.043753102966384	22.436566683896856
36	18.76490190029237	25.522881759358956	35.32570165348523	20.386514686863443
37	16.545680772947648	27.813611577052654	37.192895713769104	18.447811936230593
38	16.419416856845732	27.235208427401314	35.39823189655348	20.94714281919947
39	17.433410085994307	26.21508588193711	35.63400626416127	20.717497767907307
40	19.37456456315341	23.79278007400128	36.10841534699081	20.724240015854498
41	15.658973012620262	25.527376591323748	35.02270911694939	23.79094127910659
42	18.198348762184573	24.027328578345944	34.251845434987366	23.522477224482124
43	17.720670710653362	24.584483431436446	34.468005990385144	23.226839867525044
44	17.260971986981332	25.6093051194093	35.07664710052691	22.053075793082453
45	16.62434033233153	27.37638701320459	33.338985925046636	22.660286729417244
46	17.923755391244477	27.081566898422928	35.237439498540205	19.757238211792398
47	15.99445092562892	25.829960506771872	37.16286206382253	21.01272650377668
48	16.554261815789527	25.118755503615276	36.994510175686635	21.332472504908562
49	17.66223789511105	23.768058498194915	37.43439077660481	21.135312830089223
50	17.357815184768242	25.49673000974561	35.42683537269308	21.71861943279307
51	16.84336123534327	23.870622391209743	34.05734179723813	25.228674576208853
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	687.0
2	1247.0
3	10631.0
4	20015.0
5	12576.0
6	5137.0
7	5007.0
8	4877.0
9	5046.5
10	5216.0
11	5253.5
12	5291.0
13	5138.0
14	4985.0
15	4743.0
16	4501.0
17	4060.0
18	3619.0
19	3450.0
20	3281.0
21	3469.0
22	3657.0
23	3870.5
24	4084.0
25	4604.0
26	6262.0
27	7400.0
28	7891.5
29	8383.0
30	9969.0
31	11555.0
32	13763.0
33	15971.0
34	17678.0
35	19385.0
36	19562.0
37	19739.0
38	21925.5
39	24112.0
40	28365.5
41	32619.0
42	35000.0
43	37381.0
44	38192.0
45	39003.0
46	45477.5
47	51952.0
48	47743.5
49	43535.0
50	39491.5
51	35448.0
52	29506.5
53	23565.0
54	19218.0
55	14871.0
56	12496.0
57	10121.0
58	8810.5
59	7500.0
60	6581.0
61	5662.0
62	4705.5
63	3749.0
64	3079.0
65	2409.0
66	1898.0
67	1387.0
68	1149.5
69	912.0
70	777.0
71	642.0
72	561.0
73	480.0
74	389.0
75	201.5
76	105.0
77	76.5
78	48.0
79	37.0
80	26.0
81	18.5
82	11.0
83	11.5
84	12.0
85	7.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489451.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.923427776349975
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35225529932886	25.90130218208217
2	9.259733165550625	6.282437785396583
3	3.6391208517286064	3.703543601490737
4	2.014143614285201	2.7330662172160203
5	1.298008213332684	2.2016443939050183
6	0.95721775124166	1.9483264350291951
7	0.7011610111491225	1.6650049444916843
8	0.5655795930202672	1.5349118780480355
9	0.46617565400830446	1.4232848516859087
>10	3.9966017283753	28.032233470995248
>50	0.6728147906021991	15.319419079529247
>100	0.07050272421847795	4.020726389549265
>500	0.0024311284213268257	0.6643136468451736
>1k	0.0036466926319902388	2.324891583434568
>5k	0.0	0.0
>10k+	6.077821053317064E-4	2.244893540301133
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10888	2.2245332014849293	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCG	3026	0.6182437057029202	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2618	0.5348850038103916	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTC	2027	0.4141374723925378	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGC	1462	0.298702015114894	No Hit
CCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1135	0.23189246727455862	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	1008	0.20594502820507057	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGCT	972	0.19858984862631807	TruSeq Adapter, Index 27 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCTGC	849	0.17345965173224695	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCT	813	0.16610447215349441	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTC	588	0.12013459978629118	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCTTGCTCGTATGCCGTCTTCT	492	0.10052078757628445	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.1293163156271E-4	0.0	0.0	0.21064417071371802	0.0
2	6.1293163156271E-4	0.0	0.0	0.8824172389064483	0.0
3	6.1293163156271E-4	0.0	0.0	1.1786675274950915	0.0
4	6.1293163156271E-4	0.0	0.0	1.5950524158700259	0.0
5	6.1293163156271E-4	0.0	0.0	2.6002602916328703	0.0
6	6.1293163156271E-4	0.0	0.0	3.014397764025408	0.0
7	6.1293163156271E-4	0.0	0.0	3.6371363016931215	0.0
8	6.1293163156271E-4	0.0	0.0	4.469088836267574	0.0
9	6.1293163156271E-4	0.0	0.0	4.8468590318540565	0.0
10	6.1293163156271E-4	0.0	0.0	6.217782781115985	0.0
11	8.172421754169467E-4	0.0	0.0	7.3939985820848255	0.0
12	8.172421754169467E-4	0.0	0.0	8.755524046329459	0.0
13	8.172421754169467E-4	0.0	0.0	9.146983048354176	0.0
14	8.172421754169467E-4	0.0	0.0	9.292043534490684	0.0
15	8.172421754169467E-4	0.0	0.0	9.567045526518488	0.0
16	8.172421754169467E-4	0.0	0.0	10.030013218892188	0.0
17	8.172421754169467E-4	0.0	0.0	10.643966403174169	0.0
18	8.172421754169467E-4	0.0	0.0	11.38663523008432	0.0
19	8.172421754169467E-4	0.0	0.0	11.903745216579392	0.0
20	8.172421754169467E-4	0.0	0.0	12.380401715391326	0.0
21	8.172421754169467E-4	0.0	0.0	13.022345444181338	0.0
22	8.172421754169467E-4	0.0	0.0	13.70576421337376	0.0
23	8.172421754169467E-4	0.0	0.0	14.349342426514605	0.0
24	8.172421754169467E-4	0.0	0.0	14.852150674939882	0.0
25	8.172421754169467E-4	0.0	0.0	15.261793315367626	0.0
26	8.172421754169467E-4	0.0	0.0	15.636090231708588	0.0
27	8.172421754169467E-4	0.0	0.0	16.04511994050477	0.0
28	8.172421754169467E-4	0.0	0.0	16.447203090809907	0.0
29	8.172421754169467E-4	0.0	0.0	16.90404146686798	0.0
30	8.172421754169467E-4	0.0	0.0	17.430958359468057	0.0
31	8.172421754169467E-4	0.0	0.0	17.91333555350791	0.0
32	8.172421754169467E-4	0.0	0.0	18.366904960864314	0.0
33	8.172421754169467E-4	0.0	0.0	18.7977958978529	0.0
34	8.172421754169467E-4	0.0	0.0	19.244010125630552	0.0
35	8.172421754169467E-4	0.0	0.0	19.72230110879332	0.0
36	8.172421754169467E-4	0.0	0.0	20.195484328359733	0.0
37	8.172421754169467E-4	0.0	0.0	20.668667547926145	0.0
38	8.172421754169467E-4	0.0	0.0	21.11590332842307	0.0
39	8.172421754169467E-4	0.0	0.0	21.57253739393729	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGAAGCG	20	7.029312E-4	45.000004	21
TCCGACC	20	7.029312E-4	45.000004	34
GGGTACG	20	7.029312E-4	45.000004	7
CCAATAG	20	7.029312E-4	45.000004	21
CGATTGA	20	7.029312E-4	45.000004	10
CACGACC	20	7.029312E-4	45.000004	27
AGGCGAA	20	7.029312E-4	45.000004	22
GCGTTGC	20	7.029312E-4	45.000004	1
CGTTCAT	20	7.029312E-4	45.000004	17
CATTACG	20	7.029312E-4	45.000004	22
AAACGCC	20	7.029312E-4	45.000004	17
ATATACG	20	7.029312E-4	45.000004	18
TGTAGCG	20	7.029312E-4	45.000004	1
CGATGAC	20	7.029312E-4	45.000004	12
CGGTAAA	20	7.029312E-4	45.000004	22
GCTGTCG	20	7.029312E-4	45.000004	1
GCCGTAA	20	7.029312E-4	45.000004	26
ACGACCA	20	7.029312E-4	45.000004	28
CGGGTCA	25	3.887513E-5	45.0	6
CGGGTAT	50	2.1827873E-11	45.0	6
>>END_MODULE