FastQCFastQC Report
Sat 18 Jun 2016
SRR3549744_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549744_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864376
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315793.6533869519746034No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG88241.020852036613696No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73740.8531009653206474No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC70770.8187409183040715No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC50320.5821540625838755No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT25360.2933908391718419No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC20700.23947911557007598No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC19000.21981174859089103Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT18790.21738225031699168No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC15820.18302220330041558Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT14640.16937073680898126No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTA13840.16011550528936483No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT13370.15467805677159013Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC13060.15109165455773876No Hit
GAATCAGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC12560.14530713485797847No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC11630.13454792821642433Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG253.8899994E-545.00000420
AATACGT253.8899994E-545.00000418
AGTCGAG253.8899994E-545.00000445
CTTAACG207.032316E-445.025
GACGTAA453.8380676E-1045.031
ACCTACG351.2114651E-745.035
TCTACGA351.2114651E-745.039
TCCCGTA207.032316E-445.018
TTACGTA207.032316E-445.035
ACGTAAT207.032316E-445.037
CCGTTGA207.032316E-445.025
TACGTAA207.032316E-445.036
CGCGGAT302.1648757E-644.9999964
ATAGCCG302.1648757E-644.99999612
TATTGCG600.044.9999961
TGCGACT302.1648757E-644.99999625
CGAGGGT302.1648757E-644.9999964
TTACGGA302.1648757E-644.99999622
CGTTTAT23150.044.6112331
TAGGGCG2400.043.1249965
Produced by FastQC (version 0.11.5)