##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549744_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	864376
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09876488935371	33.0	31.0	34.0	30.0	34.0
2	32.31247628346923	34.0	31.0	34.0	30.0	34.0
3	32.272756300498855	33.0	31.0	34.0	30.0	34.0
4	35.81996029505678	37.0	35.0	37.0	35.0	37.0
5	35.89076397308579	37.0	35.0	37.0	35.0	37.0
6	28.93608452802947	37.0	33.0	37.0	0.0	37.0
7	32.46363619535943	37.0	35.0	37.0	17.0	37.0
8	34.84699945394134	37.0	33.0	37.0	32.0	37.0
9	37.3416210075245	39.0	37.0	39.0	34.0	39.0
10	37.18279429322425	39.0	37.0	39.0	34.0	39.0
11	37.27511407072848	39.0	37.0	39.0	34.0	39.0
12	37.27862411728229	39.0	37.0	39.0	34.0	39.0
13	37.254238896035986	39.0	37.0	39.0	34.0	39.0
14	38.45740626764279	40.0	38.0	41.0	34.0	41.0
15	38.54078549149907	40.0	38.0	41.0	34.0	41.0
16	38.60403111608837	40.0	38.0	41.0	34.0	41.0
17	38.534401695558415	40.0	38.0	41.0	34.0	41.0
18	38.35942113154461	40.0	38.0	41.0	34.0	41.0
19	38.1649930123002	40.0	37.0	41.0	34.0	41.0
20	37.916406748914824	40.0	35.0	41.0	34.0	41.0
21	37.83610026192305	39.0	35.0	41.0	34.0	41.0
22	37.82458791081659	39.0	35.0	41.0	34.0	41.0
23	37.70602145362666	39.0	35.0	41.0	34.0	41.0
24	37.59153655353688	39.0	35.0	41.0	33.0	41.0
25	37.40484002332318	39.0	35.0	41.0	33.0	41.0
26	37.408451877423715	39.0	35.0	41.0	33.0	41.0
27	37.42109336677557	39.0	35.0	41.0	33.0	41.0
28	37.314888428184034	39.0	35.0	41.0	33.0	41.0
29	37.267782770461004	39.0	35.0	41.0	33.0	41.0
30	37.0270634538673	39.0	35.0	41.0	33.0	41.0
31	36.903176395457535	39.0	35.0	41.0	33.0	41.0
32	36.53336973724398	39.0	35.0	41.0	31.0	41.0
33	36.099339870611864	39.0	35.0	41.0	30.0	41.0
34	35.68314599202199	39.0	35.0	41.0	25.0	41.0
35	35.456871778022524	39.0	35.0	41.0	24.0	41.0
36	35.25679681064722	39.0	35.0	41.0	23.0	41.0
37	35.225646015160066	39.0	35.0	41.0	22.0	41.0
38	35.11642155728526	39.0	35.0	41.0	21.0	41.0
39	35.0305121845123	39.0	35.0	41.0	21.0	41.0
40	34.94905573500421	39.0	35.0	41.0	21.0	41.0
41	34.835594694901296	39.0	35.0	41.0	20.0	41.0
42	34.84892454209742	39.0	35.0	41.0	20.0	41.0
43	34.7745992484752	39.0	35.0	41.0	19.0	41.0
44	34.70391588845595	39.0	35.0	41.0	18.0	41.0
45	34.623824585597006	38.0	35.0	41.0	20.0	41.0
46	34.50148777846678	38.0	34.0	40.0	19.0	41.0
47	34.4555274556443	38.0	34.0	40.0	20.0	41.0
48	34.42115352578045	38.0	34.0	40.0	20.0	41.0
49	34.39197872222274	38.0	34.0	40.0	19.0	41.0
50	34.3063828704175	38.0	34.0	40.0	18.0	41.0
51	33.35356719760845	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	38.0
10	44.0
11	21.0
12	20.0
13	24.0
14	20.0
15	36.0
16	65.0
17	128.0
18	249.0
19	503.0
20	984.0
21	1711.0
22	2671.0
23	4171.0
24	6877.0
25	11874.0
26	17941.0
27	21150.0
28	19928.0
29	17342.0
30	16662.0
31	18770.0
32	23026.0
33	32382.0
34	50108.0
35	74678.0
36	72875.0
37	102030.0
38	157449.0
39	210455.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.316417855192647	3.4885281405314355	47.489287069516045	27.705766934759872
2	28.537118106009423	8.518283709867003	46.5564754227327	16.38812276139088
3	15.152317972734089	8.174567549307246	59.8503429063278	16.822771571630867
4	14.626736512813867	3.7320564198913435	62.269429044767556	19.371778022527234
5	17.133978731477967	4.568266587688691	57.522999250326244	20.774755430507096
6	14.284408636982054	26.92103899229039	48.33151313780114	10.463039232926413
7	69.31751922774349	2.1185224948402084	25.114764870843242	3.449193406573065
8	69.74279711606985	5.488352291132563	20.928392273732726	3.840458319064852
9	64.7491369496608	4.877159939655891	22.267739965015224	8.10596314566809
10	31.23721621146353	25.129804622062622	30.061454737290255	13.571524429183595
11	23.499842661064164	21.387914518681686	39.19127786981591	15.920964950438234
12	21.98915749627477	18.808250113376587	42.016668671966826	17.185923718381815
13	18.57432413671828	21.80451562745842	43.7475126565291	15.873647579294195
14	15.057220468870028	25.35250863050339	42.35888085740464	17.231390043221932
15	13.96787971901117	22.21012614880561	47.677283959758256	16.144710172424965
16	14.681573759567595	22.033929678750912	44.03627587994114	19.248220681740356
17	14.342369524373652	22.119656260701362	42.87104223162142	20.666931983303563
18	15.198709820726167	21.76784177256194	44.65510379742149	18.3783446092904
19	16.691000212870325	22.471355058446786	41.84903329106778	18.988611437615113
20	18.554656769739093	22.641419937619737	43.11746277083121	15.686460521809952
21	18.15124436587781	24.70533656649421	41.478245578313135	15.665173489314835
22	16.876567604838634	21.45698168389682	40.96134089794256	20.705109813321982
23	15.891463899969457	23.752857552731683	41.61152091219562	18.744157635103242
24	17.196567234629374	21.61779133155016	42.811114607531906	18.37452682628856
25	14.92059011356169	26.03311521837719	40.39920127351986	18.647093394541265
26	14.816005997390025	24.624584671485557	40.89389339824336	19.66551593288106
27	15.333142058548594	25.512971206974743	40.28860125686044	18.865285477616222
28	13.497366886632669	25.294663433505786	43.173572611918885	18.034397067942653
29	15.944797171601246	23.57041380140124	41.02138421242608	19.463404814571437
30	15.203453126879968	25.371250474330616	41.437059798050846	17.988236600738567
31	16.916018029190997	25.267823262098904	40.26002572954363	17.556132979166474
32	19.137389284292947	25.699464122095012	38.173896545022075	16.989250048589966
33	16.85828852258739	26.02628948513147	37.34752006071432	19.76790193156682
34	16.377016483567335	26.34524790137625	38.726086795561194	18.55164881949522
35	16.77510712930484	25.939753070423055	36.14040648976834	21.144733310503764
36	16.68625690671652	27.118522494840207	36.26037742834137	19.9348431701019
37	17.03228687515618	26.6820226382963	37.547664442326024	18.738026044221495
38	16.517233241089528	27.346316880616765	36.204267587253696	19.93218229104001
39	16.78783307264431	27.13957814654734	37.90109859598138	18.171490184826975
40	17.698316473386583	26.168472979351577	37.7075485668274	18.42566198043444
41	15.562093348265108	26.401242052069936	36.501244828639386	21.53541977102557
42	17.219705313428417	24.67884346626931	37.57114959230705	20.53030162799522
43	16.708122391181615	25.18151822817848	35.61586624339408	22.494493137245826
44	16.783899599248475	25.520259701796437	35.9675650411395	21.72827565781558
45	16.536090775310743	26.375558784602998	35.33739946504762	21.75095097503864
46	18.33206845169232	27.156816015252623	35.386683572889574	19.124431960165484
47	14.881139689209325	25.954677131248438	39.0974529602858	20.066730219256435
48	16.761224282025413	24.72083907928957	37.55738243542162	20.960554203263396
49	16.946097531629754	23.510717558099717	39.61644006774829	19.926744842522233
50	17.65759345470027	24.141808657343564	36.74940072375911	21.451197164197062
51	15.846113265523337	23.93599544642609	36.37641489351856	23.84147639453201
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	455.0
1	1376.5
2	2298.0
3	28178.5
4	54059.0
5	33092.5
6	12126.0
7	11568.5
8	11011.0
9	11095.5
10	11180.0
11	11160.5
12	11141.0
13	10504.0
14	9867.0
15	9387.5
16	8908.0
17	8227.5
18	7547.0
19	7614.5
20	7682.0
21	7335.0
22	6988.0
23	8221.0
24	9454.0
25	10132.5
26	11859.5
27	12908.0
28	15846.0
29	18784.0
30	22751.5
31	26719.0
32	27368.5
33	28018.0
34	31262.0
35	34506.0
36	36776.0
37	39046.0
38	40680.0
39	42314.0
40	45331.5
41	48349.0
42	53057.0
43	57765.0
44	60452.5
45	63140.0
46	79193.5
47	95247.0
48	81587.0
49	67927.0
50	61195.5
51	54464.0
52	44887.0
53	35310.0
54	29112.0
55	22914.0
56	19318.5
57	15723.0
58	13832.5
59	11942.0
60	10301.0
61	8660.0
62	7241.0
63	5822.0
64	4896.5
65	3971.0
66	3285.0
67	2599.0
68	2133.5
69	1668.0
70	1398.5
71	1129.0
72	969.0
73	809.0
74	666.0
75	408.0
76	293.0
77	229.0
78	165.0
79	119.0
80	73.0
81	60.0
82	47.0
83	27.5
84	8.0
85	5.0
86	2.0
87	2.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	864376.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.610722185388106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05839585379303	25.563776129081116
2	9.603084762562379	6.455332281544355
3	3.747293409395043	3.7784771319093773
4	2.1129459590830386	2.8407055849391365
5	1.3265547210342443	2.2293231096198505
6	0.9313718531235965	1.8782448363976376
7	0.7315409686560608	1.7211334185309994
8	0.5574606155930248	1.498932310399407
9	0.4705374740084766	1.4233593885041864
>10	3.871884823577229	27.58780984670917
>50	0.5075780188664061	11.37567375633495
>100	0.07300779258360954	4.021734505211269
>500	0.0027812492412803043	0.538911021501369
>1k	0.003824217706760419	2.0889228819123966
>5k	0.0013906246206401521	3.307665716747453
>10k+	3.4765615516003804E-4	3.689998080657357
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31579	3.6533869519746034	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	8824	1.020852036613696	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7374	0.8531009653206474	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	7077	0.8187409183040715	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	5032	0.5821540625838755	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	2536	0.2933908391718419	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC	2070	0.23947911557007598	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1900	0.21981174859089103	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT	1879	0.21738225031699168	No Hit
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1582	0.18302220330041558	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT	1464	0.16937073680898126	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTA	1384	0.16011550528936483	No Hit
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	1337	0.15467805677159013	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCC	1306	0.15109165455773876	No Hit
GAATCAGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	1256	0.14530713485797847	No Hit
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1163	0.13454792821642433	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3138078799041158E-4	0.0	0.0	0.1677510712930484	0.0
2	2.3138078799041158E-4	0.0	0.0	0.7681842161281665	0.0
3	2.3138078799041158E-4	0.0	0.0	1.010786972336113	0.0
4	2.3138078799041158E-4	0.0	0.0	1.496455246327987	0.0
5	2.3138078799041158E-4	0.0	0.0	2.848181809767971	0.0
6	2.3138078799041158E-4	0.0	0.0	3.237595675955834	0.0
7	2.3138078799041158E-4	0.0	0.0	3.866720038501763	0.0
8	2.3138078799041158E-4	0.0	0.0	4.645085009301508	0.0
9	2.3138078799041158E-4	0.0	0.0	4.940789656353254	0.0
10	2.3138078799041158E-4	0.0	0.0	6.5498116560385755	0.0
11	2.3138078799041158E-4	0.0	0.0	7.710996140568456	0.0
12	2.3138078799041158E-4	0.0	0.0	9.222144066933835	0.0
13	2.3138078799041158E-4	0.0	0.0	9.588882615898637	0.0
14	3.470711819856174E-4	0.0	0.0	9.731528871694726	0.0
15	3.470711819856174E-4	0.0	0.0	10.06587411034087	0.0
16	3.470711819856174E-4	0.0	0.0	10.516256814164207	0.0
17	3.470711819856174E-4	0.0	0.0	11.057687858061769	0.0
18	4.6276157598082316E-4	0.0	0.0	11.691439836367508	0.0
19	4.6276157598082316E-4	0.0	0.0	12.343008135348505	0.0
20	4.6276157598082316E-4	0.0	0.0	12.82289188964062	0.0
21	4.6276157598082316E-4	0.0	0.0	13.495515840328746	0.0
22	4.6276157598082316E-4	0.0	0.0	14.245768045387655	0.0
23	4.6276157598082316E-4	0.0	0.0	14.96258572658195	0.0
24	4.6276157598082316E-4	0.0	0.0	15.534559034494247	0.0
25	4.6276157598082316E-4	0.0	0.0	16.012707432876432	0.0
26	4.6276157598082316E-4	0.0	0.0	16.439257915536757	0.0
27	4.6276157598082316E-4	0.0	0.0	16.903176395457532	0.0
28	4.6276157598082316E-4	0.0	0.0	17.324983571964054	0.0
29	4.6276157598082316E-4	0.0	0.0	17.829972141753125	0.0
30	4.6276157598082316E-4	0.0	0.0	18.365965737132914	0.0
31	4.6276157598082316E-4	0.0	0.0	18.87477208992383	0.0
32	4.6276157598082316E-4	0.0	0.0	19.406716521513786	0.0
33	4.6276157598082316E-4	0.0	0.0	19.911820781696854	0.0
34	4.6276157598082316E-4	0.0	0.0	20.408595333512267	0.0
35	4.6276157598082316E-4	0.0	0.0	20.963330772719278	0.0
36	5.78451969976029E-4	0.0	0.0	21.462881893990577	0.0
37	5.78451969976029E-4	0.0	0.0	22.004891389858116	0.0
38	5.78451969976029E-4	0.0	0.0	22.50918581728322	0.0
39	5.78451969976029E-4	0.0	0.0	23.046220626208964	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	25	3.8899994E-5	45.000004	20
AATACGT	25	3.8899994E-5	45.000004	18
AGTCGAG	25	3.8899994E-5	45.000004	45
CTTAACG	20	7.032316E-4	45.0	25
GACGTAA	45	3.8380676E-10	45.0	31
ACCTACG	35	1.2114651E-7	45.0	35
TCTACGA	35	1.2114651E-7	45.0	39
TCCCGTA	20	7.032316E-4	45.0	18
TTACGTA	20	7.032316E-4	45.0	35
ACGTAAT	20	7.032316E-4	45.0	37
CCGTTGA	20	7.032316E-4	45.0	25
TACGTAA	20	7.032316E-4	45.0	36
CGCGGAT	30	2.1648757E-6	44.999996	4
ATAGCCG	30	2.1648757E-6	44.999996	12
TATTGCG	60	0.0	44.999996	1
TGCGACT	30	2.1648757E-6	44.999996	25
CGAGGGT	30	2.1648757E-6	44.999996	4
TTACGGA	30	2.1648757E-6	44.999996	22
CGTTTAT	2315	0.0	44.611233	1
TAGGGCG	240	0.0	43.124996	5
>>END_MODULE