FastQCFastQC Report
Sat 18 Jun 2016
SRR3549743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653558
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57570.3481583349359382No Hit
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC45490.2751037459829047TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT44910.27159615810270943TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC44130.2668790571603778No Hit
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC33630.2033796213982213TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC27290.16503805732850013TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC16910.10226432940362538No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.034187E-445.021
ATATCCG207.034187E-445.016
GTATCGA253.8915514E-545.038
ACGGGAT1750.041.1428575
TACGGGT556.184564E-1140.9090924
TACGGGA3500.040.54
CGTTTTT35550.040.1898731
CTACGGG1700.039.7058833
GCGCGAC4500.039.09
TTTGGGA150900.038.7524874
ACAACGT356.249953E-638.57142625
CACGATA356.249953E-638.57142626
ACGATAC356.249953E-638.57142627
ACGACTC356.249953E-638.57142636
TTGGGAT91550.038.2168245
TTGGGAC53300.038.2035645
TAGGGCG1300.038.076925
CGTGGCG3550.038.02816827
TTTTGCG17050.037.87391
TTTGGGC33500.037.746274
Produced by FastQC (version 0.11.5)