##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695777
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.452642154023486	33.0	31.0	34.0	31.0	34.0
2	32.589477663101825	34.0	31.0	34.0	31.0	34.0
3	32.619000053177956	34.0	31.0	34.0	31.0	34.0
4	36.0629224593512	37.0	35.0	37.0	35.0	37.0
5	36.0811912437462	37.0	35.0	37.0	35.0	37.0
6	35.9117547720031	37.0	35.0	37.0	35.0	37.0
7	36.024497791677504	37.0	35.0	37.0	35.0	37.0
8	36.002349890841465	37.0	35.0	37.0	35.0	37.0
9	37.90469791326819	39.0	38.0	39.0	35.0	39.0
10	37.462513132799735	39.0	37.0	39.0	35.0	39.0
11	37.35470272802924	39.0	37.0	39.0	35.0	39.0
12	37.18615878363326	39.0	37.0	39.0	34.0	39.0
13	37.13793212480436	39.0	37.0	39.0	33.0	39.0
14	38.28926509499452	40.0	38.0	41.0	34.0	41.0
15	38.35085666815661	40.0	38.0	41.0	34.0	41.0
16	38.42448226946277	40.0	38.0	41.0	34.0	41.0
17	38.43084062853472	40.0	38.0	41.0	34.0	41.0
18	38.426320502114905	40.0	38.0	41.0	34.0	41.0
19	38.38454562309476	40.0	37.0	41.0	34.0	41.0
20	38.31396841229302	40.0	37.0	41.0	34.0	41.0
21	38.250249720815724	40.0	37.0	41.0	34.0	41.0
22	38.14158846871914	40.0	37.0	41.0	34.0	41.0
23	37.94265116553149	40.0	36.0	41.0	33.0	41.0
24	37.6560823367257	40.0	36.0	41.0	33.0	41.0
25	37.612414609853445	39.0	36.0	41.0	33.0	41.0
26	37.72492910803318	40.0	36.0	41.0	33.0	41.0
27	37.77952274938666	40.0	36.0	41.0	33.0	41.0
28	37.730461052894825	40.0	36.0	41.0	33.0	41.0
29	37.730854857231556	40.0	36.0	41.0	33.0	41.0
30	37.62793395010183	40.0	36.0	41.0	33.0	41.0
31	37.5094592089132	40.0	36.0	41.0	33.0	41.0
32	37.41322147757112	40.0	35.0	41.0	33.0	41.0
33	37.287952892952774	40.0	35.0	41.0	33.0	41.0
34	37.16932724134313	39.0	35.0	41.0	32.0	41.0
35	37.10101368685657	39.0	35.0	41.0	32.0	41.0
36	37.00153210008379	39.0	35.0	41.0	31.0	41.0
37	36.91877282520118	39.0	35.0	41.0	31.0	41.0
38	36.80848461504189	39.0	35.0	41.0	31.0	41.0
39	36.80052373102301	39.0	35.0	41.0	31.0	41.0
40	36.7347339736726	39.0	35.0	41.0	31.0	41.0
41	36.7030657811339	39.0	35.0	41.0	31.0	41.0
42	36.67769558349874	39.0	35.0	41.0	31.0	41.0
43	36.52587969996134	39.0	35.0	41.0	31.0	41.0
44	36.38332827903193	39.0	35.0	41.0	31.0	41.0
45	36.3098880819573	39.0	35.0	41.0	31.0	41.0
46	36.16954857662728	38.0	35.0	40.0	30.0	41.0
47	36.068096530928734	38.0	35.0	40.0	30.0	41.0
48	35.99664978865355	38.0	35.0	40.0	30.0	41.0
49	35.95042377083462	38.0	35.0	40.0	30.0	41.0
50	35.80729889030537	38.0	35.0	40.0	29.0	41.0
51	34.984526651498975	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	10.0
11	4.0
12	5.0
13	12.0
14	15.0
15	24.0
16	50.0
17	68.0
18	129.0
19	258.0
20	451.0
21	706.0
22	1164.0
23	1720.0
24	2595.0
25	3874.0
26	5187.0
27	6213.0
28	7083.0
29	8174.0
30	10047.0
31	12364.0
32	16280.0
33	22961.0
34	41286.0
35	55058.0
36	56830.0
37	84740.0
38	150036.0
39	208282.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.28004949861809	4.336590603023669	54.6348902018894	16.74846969646884
2	20.237806078671756	4.222042407265547	54.31639735145025	21.223754162612448
3	18.94342583902601	4.080330335725383	55.88600945417856	21.09023437107004
4	17.47240854469176	4.39652359879674	54.34902274723079	23.782045109280702
5	16.445786509183257	5.172921783847411	54.12783118729133	24.253460519678
6	21.478002290963914	6.302162905643619	53.971602970491986	18.248231832900483
7	83.40517148454174	1.9657160268304357	10.22310309193894	4.406009396688882
8	85.30075009665453	2.4131294940764065	7.935444833617668	4.350675575651394
9	80.31653820117653	4.813036360788011	10.131550769858734	4.738874668176729
10	38.02425202327757	27.178248203088057	18.801426319064873	15.996073454569496
11	28.57955925533612	24.935431898438722	25.420357384621795	21.064651461603358
12	25.164815738375946	21.757402156150608	31.398997092459226	21.678785013014227
13	23.416410717801824	23.285621686258672	33.162924327765936	20.135043268173565
14	21.437472063606585	25.366029633057718	31.503628317693742	21.692869985641952
15	18.72108448540265	25.475260033027823	34.55388723685894	21.24976824471059
16	21.569554613044122	24.0117163976389	32.245963864859	22.172765124457978
17	21.375239480465723	25.225898527832914	29.59971370137271	23.799148290328652
18	22.67738082747777	23.810071330325663	32.12925980594357	21.383288036252996
19	22.802995787443393	26.02026223919445	29.770745511852216	21.40599646150994
20	24.68664529008576	25.811430961356873	29.95629346759091	19.54563028096646
21	24.764974984801164	26.375692211728758	29.81127573920955	19.048057064260533
22	23.131261884195656	24.31152510071474	29.529432562444573	23.02778045264503
23	21.887760015062298	25.937908266585413	28.044474019693087	24.129857698659197
24	22.35903170124911	25.815024066619046	29.904121579184135	21.92182265294771
25	22.41795862754877	26.11569511495781	27.08252787890373	24.38381837858969
26	22.170178088669214	27.251834998857394	27.622068565071856	22.95591834740154
27	21.393923627829032	26.64603745165477	29.741425772912873	22.218613147603328
28	19.939578341911275	25.506160738282524	32.12164242278776	22.432618497018442
29	22.10133419184595	24.401640180690077	31.218192035666597	22.278833591797373
30	24.431247368050396	25.07311969208525	30.168430402269692	20.327202537594662
31	23.69020533877952	26.4568963906539	28.67197392267925	21.180924347887327
32	22.71259325904708	26.06007384549935	28.45954953957949	22.76778335587408
33	23.232012555747026	25.599581475099065	28.96459641523074	22.20380955392317
34	21.664556316176018	25.103876673129466	30.003578732841124	23.22798827785339
35	22.671344410637317	24.725881999548708	28.85723442999697	23.74553915981701
36	24.45956103751633	26.634395790605325	28.248274950163633	20.65776822171472
37	21.355118090997546	27.303000817790757	31.213880309351993	20.128000781859708
38	20.51619987438504	27.753863666088417	30.53564576006393	21.194290699462613
39	22.258568478118708	24.89791987950162	31.230264869347508	21.613246773032163
40	22.322094579153955	24.43139109226088	30.3924964464189	22.854017882166268
41	19.60311996516125	24.962020877378816	29.048675078365626	26.386184079094306
42	20.931131670060953	24.783946580585447	29.275471882514083	25.009449866839518
43	21.959190947674326	23.5888797703862	30.612538212674462	23.83939106926501
44	21.39104914361929	24.374763753329013	29.419052368790577	24.81513473426112
45	23.14908368629604	24.15874626496708	28.2662404764745	24.42592957226238
46	23.225401242064628	24.47436463119649	30.368925675899032	21.931308450839854
47	20.09595028292111	25.426250077251762	32.40075483955348	22.07704480027365
48	20.66337346592371	23.65987953036677	32.97981968360552	22.696927320104
49	22.26891662127377	22.831884353751274	32.60786142686521	22.291337598109738
50	21.541240943578185	22.98006401476335	30.63740250108871	24.84129254056975
51	20.92466408058904	22.912657360044957	28.989316979434506	27.1733615799315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	429.0
1	1272.5
2	2116.0
3	3075.0
4	4034.0
5	2909.0
6	1784.0
7	1914.5
8	2045.0
9	2202.5
10	2360.0
11	2638.5
12	2917.0
13	3116.5
14	3316.0
15	3146.0
16	2976.0
17	3176.0
18	3376.0
19	3092.5
20	2809.0
21	2884.5
22	2960.0
23	3504.0
24	4048.0
25	4272.0
26	6175.0
27	7854.0
28	8931.0
29	10008.0
30	10931.0
31	11854.0
32	13807.5
33	15761.0
34	17318.0
35	18875.0
36	20517.5
37	22160.0
38	24038.0
39	25916.0
40	29488.0
41	33060.0
42	36098.0
43	39136.0
44	42233.5
45	45331.0
46	51695.5
47	58060.0
48	65443.5
49	72827.0
50	70463.0
51	68099.0
52	59262.5
53	50426.0
54	43847.0
55	37268.0
56	34768.0
57	32268.0
58	29078.0
59	25888.0
60	23615.0
61	21342.0
62	19871.5
63	18401.0
64	16371.0
65	14341.0
66	12079.0
67	9817.0
68	8085.0
69	6353.0
70	5369.5
71	4386.0
72	3556.0
73	2726.0
74	2474.0
75	1559.5
76	897.0
77	656.5
78	416.0
79	341.0
80	266.0
81	178.0
82	90.0
83	58.5
84	27.0
85	25.0
86	23.0
87	15.0
88	7.0
89	4.0
90	1.0
91	1.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.48674413297565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.25955852842428	23.79964533207674
2	10.822691541027742	7.031880218469764
3	3.967542620102322	3.8667762580781995
4	2.0366629616385397	2.6465819407943854
5	1.2391813962951634	2.012848447789224
6	0.9192679552669765	1.7918413711441181
7	0.6881198912769562	1.564834238850785
8	0.5283360481427363	1.3731134409792465
9	0.4695917340256709	1.3729955859227094
>10	5.6482092762480525	40.598035867084704
>50	0.35488581622648957	7.313608248713124
>100	0.05877375716723389	3.402464644634514
>500	0.004486546348643808	1.0317874922621542
>1k	0.002691927809186285	2.19358691320037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	3535	0.508065084071477	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	3321	0.477308103027263	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2391	0.34364458727437097	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2053	0.29506580412977146	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2024	0.290897802025649	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	1726	0.24806798730052879	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	939	0.13495703364727493	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	902	0.12963923785925663	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	877	0.1260461325970821	No Hit
GCTCTGGAGCCGCAGAGAGGACAGGCAGAATTTGAAACCTAAAGAACTCCC	833	0.11972226733565496	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8744842097396146E-4	0.0	0.0	0.5362350293269251	0.0
2	2.8744842097396146E-4	0.0	0.0	1.7481175721531468	0.0
3	2.8744842097396146E-4	0.0	0.0	2.3040428183167885	0.0
4	2.8744842097396146E-4	0.0	0.0	2.874915382371076	0.0
5	2.8744842097396146E-4	0.0	0.0	3.7950377779087265	0.0
6	2.8744842097396146E-4	0.0	0.0	4.347657367231167	0.0
7	2.8744842097396146E-4	0.0	0.0	4.843505893411251	0.0
8	2.8744842097396146E-4	0.0	0.0	5.690328941600542	0.0
9	2.8744842097396146E-4	0.0	0.0	6.099080596225515	0.0
10	2.8744842097396146E-4	0.0	0.0	7.095089374900291	0.0
11	2.8744842097396146E-4	0.0	0.0	9.231262315368285	0.0
12	4.3117263146094225E-4	0.0	0.0	10.845428923347567	0.0
13	4.3117263146094225E-4	0.0	0.0	11.376633605307447	0.0
14	4.3117263146094225E-4	0.0	0.0	11.54924638210231	0.0
15	4.3117263146094225E-4	0.0	0.0	11.822609830448549	0.0
16	4.3117263146094225E-4	0.0	0.0	12.432000482913347	0.0
17	4.3117263146094225E-4	0.0	0.0	13.332432661614282	0.0
18	4.3117263146094225E-4	0.0	0.0	14.314212743450847	0.0
19	5.748968419479229E-4	0.0	0.0	15.001214469578615	0.0
20	5.748968419479229E-4	0.0	0.0	15.682610951497391	0.0
21	5.748968419479229E-4	0.0	0.0	16.6904051154321	0.0
22	5.748968419479229E-4	0.0	0.0	17.71860811725596	0.0
23	5.748968419479229E-4	0.0	0.0	18.79438383275101	0.0
24	5.748968419479229E-4	0.0	0.0	19.583429748324534	0.0
25	5.748968419479229E-4	0.0	0.0	20.250022636563152	0.0
26	5.748968419479229E-4	0.0	0.0	20.853951769029443	0.0
27	5.748968419479229E-4	0.0	0.0	21.441783789921196	0.0
28	5.748968419479229E-4	0.0	0.0	22.0743140402744	0.0
29	5.748968419479229E-4	0.0	0.0	22.754704452719764	0.0
30	5.748968419479229E-4	0.0	0.0	23.438831694637795	0.0
31	5.748968419479229E-4	0.0	0.0	24.093351749195502	0.0
32	5.748968419479229E-4	0.0	0.0	24.731056071126236	0.0
33	5.748968419479229E-4	0.0	0.0	25.378389915159598	0.0
34	5.748968419479229E-4	0.0	0.0	25.92569170869402	0.0
35	5.748968419479229E-4	0.0	0.0	26.526746356950575	0.0
36	5.748968419479229E-4	0.0	0.0	27.113141135737457	0.0
37	5.748968419479229E-4	0.0	0.0	27.727418411358812	0.0
38	5.748968419479229E-4	0.0	0.0	28.364979009079057	0.0
39	5.748968419479229E-4	0.0	0.0	29.019211615215795	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	25	3.8892118E-5	45.000004	23
TATTGCG	50	2.1827873E-11	45.000004	1
CTCGGTA	25	3.8892118E-5	45.000004	25
GCGATAT	25	3.8892118E-5	45.000004	9
TACGTAC	25	3.8892118E-5	45.000004	45
GATCGGA	20	7.0313655E-4	45.0	30
CAATTCG	20	7.0313655E-4	45.0	1
GTCGTAC	20	7.0313655E-4	45.0	43
ATGGTCG	20	7.0313655E-4	45.0	44
CGGTATG	20	7.0313655E-4	45.0	14
ATCGGGT	20	7.0313655E-4	45.0	10
ATTTCGA	20	7.0313655E-4	45.0	12
CAGACGA	20	7.0313655E-4	45.0	31
GCCGAAC	30	2.1642627E-6	44.999996	21
GGCGATA	95	0.0	42.63158	8
TCGTTCA	75	0.0	42.0	16
ACACGAC	65	0.0	41.53846	26
ACATACG	65	0.0	41.53846	17
TTACGGG	120	0.0	41.249996	3
TACGGGA	115	0.0	41.086956	4
>>END_MODULE