##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549737_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1298538
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53195901852699	33.0	31.0	34.0	31.0	34.0
2	32.6690470359743	34.0	31.0	34.0	31.0	34.0
3	32.705018258995885	34.0	31.0	34.0	31.0	34.0
4	36.10228811170717	37.0	35.0	37.0	35.0	37.0
5	36.13395295324434	37.0	35.0	37.0	35.0	37.0
6	35.954913910875156	37.0	35.0	37.0	35.0	37.0
7	36.075687426937066	37.0	35.0	37.0	35.0	37.0
8	36.06210214872418	37.0	35.0	37.0	35.0	37.0
9	37.94009955811844	39.0	39.0	39.0	35.0	39.0
10	37.55924893996171	39.0	37.0	39.0	35.0	39.0
11	37.46794702965951	39.0	37.0	39.0	35.0	39.0
12	37.35547130696214	39.0	37.0	39.0	35.0	39.0
13	37.348793027235246	39.0	37.0	39.0	34.0	39.0
14	38.54960655752854	40.0	38.0	41.0	34.0	41.0
15	38.63604684653048	40.0	38.0	41.0	35.0	41.0
16	38.687780411508946	40.0	38.0	41.0	35.0	41.0
17	38.67541342648425	40.0	38.0	41.0	35.0	41.0
18	38.67311699773129	40.0	38.0	41.0	35.0	41.0
19	38.65883632207914	40.0	38.0	41.0	35.0	41.0
20	38.59032234713193	40.0	38.0	41.0	35.0	41.0
21	38.52642741298291	40.0	38.0	41.0	34.0	41.0
22	38.41581840500625	40.0	38.0	41.0	34.0	41.0
23	38.208489085417604	40.0	37.0	41.0	34.0	41.0
24	37.96679111431472	40.0	37.0	41.0	33.0	41.0
25	37.915584295569325	40.0	37.0	41.0	33.0	41.0
26	38.025167534565796	40.0	37.0	41.0	34.0	41.0
27	38.08904860697184	40.0	37.0	41.0	34.0	41.0
28	38.08195986563351	40.0	37.0	41.0	34.0	41.0
29	38.06069826219949	40.0	37.0	41.0	34.0	41.0
30	37.962885953279766	40.0	37.0	41.0	34.0	41.0
31	37.89454370992608	40.0	37.0	41.0	33.0	41.0
32	37.774233022060194	40.0	37.0	41.0	33.0	41.0
33	37.62281889324764	40.0	36.0	41.0	33.0	41.0
34	37.5434303809361	40.0	36.0	41.0	33.0	41.0
35	37.45368406623449	40.0	36.0	41.0	33.0	41.0
36	37.35935259499529	40.0	36.0	41.0	32.0	41.0
37	37.34125531944387	40.0	36.0	41.0	33.0	41.0
38	37.24979399909745	40.0	35.0	41.0	32.0	41.0
39	37.196510999293054	40.0	35.0	41.0	32.0	41.0
40	37.11853330437769	39.0	35.0	41.0	32.0	41.0
41	37.068033434524054	39.0	35.0	41.0	32.0	41.0
42	37.02147723054697	39.0	35.0	41.0	32.0	41.0
43	36.91987142463293	39.0	35.0	41.0	31.0	41.0
44	36.8144436281418	39.0	35.0	41.0	31.0	41.0
45	36.75481965102292	39.0	35.0	41.0	31.0	41.0
46	36.63457134100042	39.0	35.0	41.0	31.0	41.0
47	36.54459014676505	39.0	35.0	41.0	31.0	41.0
48	36.458554928696735	39.0	35.0	41.0	31.0	41.0
49	36.43268660601384	39.0	35.0	41.0	31.0	41.0
50	36.29528515915591	39.0	35.0	41.0	31.0	41.0
51	35.48955825705524	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	9.0
10	10.0
11	12.0
12	14.0
13	11.0
14	14.0
15	31.0
16	63.0
17	118.0
18	211.0
19	401.0
20	724.0
21	1196.0
22	1842.0
23	2782.0
24	4008.0
25	5977.0
26	8227.0
27	10191.0
28	11569.0
29	13579.0
30	16585.0
31	20868.0
32	27109.0
33	38012.0
34	65911.0
35	88955.0
36	97723.0
37	149680.0
38	277547.0
39	454794.0
40	360.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.75137423779666	4.555199770819183	52.45861114576547	18.23481484561869
2	19.228008729817684	4.528015352650442	54.8931182606901	21.35085765684177
3	20.71675992539302	4.565057010268471	53.544447678851135	21.173735385487372
4	18.081180527639546	4.7434884462372295	53.639400618233736	23.535930407889488
5	17.437225556741502	5.938986768196234	52.35703537362788	24.266752301434384
6	21.915107605630332	6.572853470595392	54.19987709254561	17.31216183122866
7	84.93089921126682	2.1320130793245946	9.098155002009953	3.8389327073986284
8	86.61232863420246	2.4914942804908287	7.141415961643018	3.7547611236636893
9	81.13201153913093	5.384132000757775	9.060343247559949	4.423513212551346
10	34.658130913381044	36.33224441641292	15.472631528688419	13.536993141517614
11	27.123811548064054	24.923259850693626	28.708901857319542	19.24402674392278
12	25.356747357412722	22.42691396016135	31.72644928373294	20.48988939869299
13	24.22370388852694	23.097206242712957	32.66188590553376	20.017203963226336
14	20.70728773435972	24.920795540831303	32.283460322300925	22.088456402508054
15	18.973183688116944	25.2752711125897	34.27531577820595	21.47622942108741
16	21.284321290559074	24.69608128526081	32.93842767789622	21.081169746283898
17	20.77675046860392	24.745521501873647	31.191077966143467	23.286650063378968
18	21.668445590348533	23.605008093717707	32.64941033685575	22.077135979078008
19	22.3652292039201	24.70547646661091	31.65452223962641	21.27477208984258
20	24.73304593319564	25.37892614617362	30.696521780648702	19.191506139982042
21	25.024450574415226	25.476497414784937	30.041169376637416	19.45788263416242
22	23.748708162564363	23.94092433182548	29.727200898240945	22.583166607369208
23	21.331990284458367	26.2113238118561	30.160842424326432	22.295843479359096
24	22.157688107702665	24.50371109663329	31.48309868482863	21.855502110835417
25	21.926736067793165	26.25606643779389	28.540173641433675	23.277023852979273
26	21.733441762967274	27.13466991339491	28.616028179383274	22.51586014425454
27	21.350010550326598	26.326992356018845	30.699833197026194	21.623163896628363
28	20.299983519927796	26.15649291741944	31.63396065421266	21.90956290844011
29	21.661976776959936	24.643021613537684	31.35533962040387	22.33966198909851
30	22.526949538634987	25.0917570375299	31.193157227589797	21.188136196245317
31	23.106216375647072	26.91111080307238	27.989169358155095	21.993503463125453
32	22.740035332042652	27.840617679266995	27.928793766528205	21.490553222162152
33	22.117412043390335	26.921661129670444	28.66369717328257	22.29722965365665
34	21.92334764173247	25.579536370903277	29.242732981245062	23.254383006119188
35	21.739294498890292	26.321986726611	29.03727114647396	22.901447628024748
36	22.26781195467518	27.459034699023054	29.04173770809942	21.231415638202346
37	22.36499817487051	27.117881802457838	29.07800926888547	21.43911075378618
38	22.196115939618245	28.20271720966194	27.835458030492756	21.765708820227054
39	21.95599974740824	27.405512969200746	28.13956926943994	22.498918013951076
40	21.735367005047216	26.432110573583522	29.560628953484613	22.271893467884652
41	19.44332780403808	27.350605065080885	29.48446637680222	23.721600754078818
42	21.04150975943715	27.712935624525425	28.44722295381421	22.79833166222321
43	22.166775250319976	26.264999561044807	29.39860058003693	22.169624608598284
44	22.550437492010246	25.435759292373422	28.811709784388288	23.202093431228043
45	22.633299911130823	24.93234699331094	28.18100047900023	24.253352616558004
46	22.163001775843295	25.833668325455246	29.254592472457485	22.748737426243977
47	20.707441753726112	26.3822853085547	30.608807751486673	22.301465186232516
48	20.685879042430795	26.023497194537242	30.574615452146954	22.716008310885012
49	22.353446722390874	25.45601283905438	29.69408673446599	22.49645370408875
50	21.336302826717432	25.053252195931115	28.759651238546734	24.850793738804715
51	20.2080339581899	25.166225401181947	28.313226105050447	26.3125145355777
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	786.0
1	2182.5
2	3579.0
3	5343.0
4	7107.0
5	5094.0
6	3081.0
7	3190.5
8	3300.0
9	3676.5
10	4053.0
11	4166.0
12	4279.0
13	4548.0
14	4817.0
15	4536.5
16	4256.0
17	4275.5
18	4295.0
19	4767.5
20	5240.0
21	5519.0
22	5798.0
23	7227.0
24	8656.0
25	10248.5
26	14405.5
27	16970.0
28	19632.5
29	22295.0
30	25353.0
31	28411.0
32	32439.0
33	36467.0
34	39715.0
35	42963.0
36	46978.0
37	50993.0
38	54745.5
39	58498.0
40	61362.5
41	64227.0
42	70870.5
43	77514.0
44	80872.5
45	84231.0
46	92129.0
47	100027.0
48	108791.0
49	117555.0
50	114414.5
51	111274.0
52	98894.5
53	86515.0
54	77582.0
55	68649.0
56	62999.0
57	57349.0
58	53994.5
59	50640.0
60	46860.0
61	43080.0
62	38905.0
63	34730.0
64	29867.5
65	25005.0
66	21460.5
67	17916.0
68	14385.0
69	10854.0
70	9414.5
71	7975.0
72	6879.0
73	5783.0
74	4977.0
75	3001.0
76	1831.0
77	1355.5
78	880.0
79	603.5
80	327.0
81	248.5
82	170.0
83	114.0
84	58.0
85	63.5
86	69.0
87	39.0
88	9.0
89	9.0
90	9.0
91	6.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1298538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.395176389727197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62594048271278	18.905793331213058
2	11.15152135460289	5.886927463371058
3	4.13480692402208	3.2741687429108444
4	1.9869390730484633	2.0978242923502095
5	1.182539451495596	1.5606668705018751
6	0.8151186029996257	1.2909119582833881
7	0.5950952661919663	1.0995351163880027
8	0.49930210057048197	1.0543333613055346
9	0.4183811944538535	0.9938920883178798
>10	6.676534008439146	43.1758196107851
>50	0.8069048244286093	13.529293731659036
>100	0.09953296585962712	4.370880486543087
>500	0.005020950798957329	1.0010608079640166
>1k	0.002362800375979919	1.758892138406989
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	4435	0.34153794498120194	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	4193	0.3229016016473912	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3659	0.2817784308198913	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCC	3021	0.23264625293984464	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	2653	0.20430668952314066	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	2407	0.1853623074565396	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.700968319756526E-5	0.0	0.0	0.34246206117957273	0.0
2	7.700968319756526E-5	0.0	0.0	1.1355077787480998	0.0
3	7.700968319756526E-5	0.0	0.0	1.575310079489395	0.0
4	7.700968319756526E-5	0.0	0.0	2.038292294873157	0.0
5	7.700968319756526E-5	0.0	0.0	2.805693787936895	0.0
6	7.700968319756526E-5	0.0	0.0	3.2886985209520243	0.0
7	7.700968319756526E-5	0.0	0.0	3.7588426368731604	0.0
8	7.700968319756526E-5	0.0	0.0	4.529940594730381	0.0
9	7.700968319756526E-5	0.0	0.0	4.874327897989893	0.0
10	7.700968319756526E-5	0.0	0.0	5.60884625632827	0.0
11	7.700968319756526E-5	0.0	0.0	7.074109498528345	0.0
12	7.700968319756526E-5	0.0	0.0	8.318817008050592	0.0
13	7.700968319756526E-5	0.0	0.0	8.69685754286744	0.0
14	7.700968319756526E-5	0.0	0.0	8.829237188284054	0.0
15	7.700968319756526E-5	0.0	0.0	9.037240342600679	0.0
16	7.700968319756526E-5	0.0	0.0	9.493984773645439	0.0
17	7.700968319756526E-5	0.0	0.0	10.194079803594503	0.0
18	7.700968319756526E-5	0.0	0.0	10.909268731450293	0.0
19	7.700968319756526E-5	0.0	0.0	11.406366236490577	0.0
20	7.700968319756526E-5	0.0	0.0	11.952287880678117	0.0
21	7.700968319756526E-5	0.0	0.0	12.723462848218535	0.0
22	7.700968319756526E-5	0.0	0.0	13.541690732192667	0.0
23	7.700968319756526E-5	0.0	0.0	14.311171486702738	0.0
24	7.700968319756526E-5	0.0	0.0	14.909921773563807	0.0
25	7.700968319756526E-5	0.0	0.0	15.446756275134035	0.0
26	7.700968319756526E-5	0.0	0.0	15.931301201813117	0.0
27	7.700968319756526E-5	0.0	0.0	16.40244644361582	0.0
28	7.700968319756526E-5	0.0	0.0	16.919797495337065	0.0
29	7.700968319756526E-5	0.0	0.0	17.483739405392836	0.0
30	7.700968319756526E-5	0.0	0.0	18.09550432871429	0.0
31	7.700968319756526E-5	0.0	0.0	18.64843385407281	0.0
32	7.700968319756526E-5	0.0	0.0	19.16786416724039	0.0
33	7.700968319756526E-5	0.0	0.0	19.67096842757008	0.0
34	7.700968319756526E-5	0.0	0.0	20.18031047223878	0.0
35	7.700968319756526E-5	0.0	0.0	20.744175372611352	0.0
36	7.700968319756526E-5	0.0	0.0	21.25944716288626	0.0
37	2.3102904959269578E-4	0.0	0.0	21.76801911072298	0.0
38	2.3102904959269578E-4	0.0	0.0	22.305315670392396	0.0
39	2.3102904959269578E-4	0.0	0.0	22.83837669748594	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGT	20	7.033626E-4	45.0	33
TAGCCCG	20	7.033626E-4	45.0	21
TGCGATA	20	7.033626E-4	45.0	41
TACGAAC	20	7.033626E-4	45.0	12
GTATACG	55	1.8189894E-12	45.0	20
TATAGCG	20	7.033626E-4	45.0	41
TATGTCG	50	1.0822987E-9	40.5	1
TTTGTCG	375	0.0	39.6	1
TTGGGAC	3350	0.0	38.88806	5
TTTGGGC	2845	0.0	38.752197	4
TTTGGGA	10450	0.0	38.648323	4
TGGGAAC	1700	0.0	38.514706	6
TGGGCGA	1840	0.0	38.274456	6
ACACGCG	620	0.0	38.10484	36
TTGGGAT	5380	0.0	38.09944	5
TATTGCG	65	9.094947E-12	38.076927	1
GCGATAT	65	9.094947E-12	38.076927	9
GGGCGAT	2645	0.0	38.024574	7
CACGACC	415	0.0	37.951805	27
TTGGGCG	1595	0.0	37.94671	5
>>END_MODULE