FastQCFastQC Report
Sat 18 Jun 2016
SRR3549734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925396
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT33900.36632965778974624No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27270.29468465392113213No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC26090.28193335609836223No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC22120.23903280325395831No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC20110.21731237221686717No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC19530.21104478515143787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGTA253.8902137E-545.00000419
GTCGAGT207.032573E-445.031
CGAGTAA207.032573E-445.015
TAATTCG207.032573E-445.022
CGCAGTA351.2115925E-745.044
AACTCGT207.032573E-445.034
GCGAGAC1050.042.85714321
TCGGCGT700.041.7857134
TTCTACG650.041.5384641
TATTCCG650.041.5384641
CGGGTAC451.927583E-840.06
TCGTTTG3100.039.9193571
TTTGGGA76300.039.574054
TCTAGCG403.457244E-739.3751
CGGGCTC2400.039.3749966
ACGACCA1100.038.86363228
TTGGGAT38050.038.67285
TCGTTCA1000.038.25000416
TCGCTTG950.037.8947371
TTGGGAC29400.037.8826525