##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925396
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62353738291499	34.0	31.0	34.0	31.0	34.0
2	32.76128922104699	34.0	31.0	34.0	31.0	34.0
3	32.78430423299863	34.0	31.0	34.0	31.0	34.0
4	36.18046760521982	37.0	35.0	37.0	35.0	37.0
5	36.20198163813114	37.0	35.0	37.0	35.0	37.0
6	36.01957972586871	37.0	36.0	37.0	35.0	37.0
7	36.15702574897666	37.0	36.0	37.0	35.0	37.0
8	36.155039572247986	37.0	37.0	37.0	35.0	37.0
9	38.043526230932486	39.0	39.0	39.0	37.0	39.0
10	37.63208075245625	39.0	37.0	39.0	35.0	39.0
11	37.49387397395277	39.0	37.0	39.0	35.0	39.0
12	37.39426904806159	39.0	37.0	39.0	35.0	39.0
13	37.39262110491076	39.0	37.0	39.0	35.0	39.0
14	38.62700508755171	40.0	38.0	41.0	35.0	41.0
15	38.716466247963034	40.0	38.0	41.0	35.0	41.0
16	38.78142978789621	40.0	38.0	41.0	35.0	41.0
17	38.78278380282603	40.0	38.0	41.0	35.0	41.0
18	38.7719073780306	40.0	38.0	41.0	35.0	41.0
19	38.7651578351322	40.0	38.0	41.0	35.0	41.0
20	38.68544817569992	40.0	38.0	41.0	35.0	41.0
21	38.62077640275082	40.0	38.0	41.0	35.0	41.0
22	38.517566533678554	40.0	38.0	41.0	34.0	41.0
23	38.33149484112747	40.0	37.0	41.0	34.0	41.0
24	38.07526399508967	40.0	37.0	41.0	34.0	41.0
25	38.024839095911375	40.0	37.0	41.0	34.0	41.0
26	38.12944080155955	40.0	37.0	41.0	34.0	41.0
27	38.20951030693887	40.0	37.0	41.0	34.0	41.0
28	38.21618852901893	40.0	37.0	41.0	34.0	41.0
29	38.20028506714963	40.0	37.0	41.0	34.0	41.0
30	38.107148723357355	40.0	37.0	41.0	34.0	41.0
31	37.99329692369537	40.0	37.0	41.0	34.0	41.0
32	37.870498683806716	40.0	37.0	41.0	34.0	41.0
33	37.735381393479116	40.0	37.0	41.0	33.0	41.0
34	37.64214347155164	40.0	36.0	41.0	33.0	41.0
35	37.596954168809894	40.0	36.0	41.0	33.0	41.0
36	37.534559258955085	40.0	36.0	41.0	33.0	41.0
37	37.481181029526816	40.0	36.0	41.0	33.0	41.0
38	37.41619587722445	40.0	36.0	41.0	33.0	41.0
39	37.36682674228114	40.0	36.0	41.0	33.0	41.0
40	37.2280126562034	40.0	36.0	41.0	32.0	41.0
41	37.1497034782947	40.0	35.0	41.0	32.0	41.0
42	37.13431331019369	40.0	35.0	41.0	32.0	41.0
43	37.0474056512023	39.0	35.0	41.0	32.0	41.0
44	36.93464419556601	39.0	35.0	41.0	31.0	41.0
45	36.89108662669819	39.0	35.0	41.0	31.0	41.0
46	36.78501095747118	39.0	35.0	41.0	31.0	41.0
47	36.710250530583664	39.0	35.0	41.0	31.0	41.0
48	36.633611988813435	39.0	35.0	41.0	31.0	41.0
49	36.58503278596406	39.0	35.0	41.0	31.0	41.0
50	36.44346312281445	39.0	35.0	41.0	31.0	41.0
51	35.632275263778965	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	6.0
11	9.0
12	7.0
13	6.0
14	20.0
15	17.0
16	40.0
17	65.0
18	135.0
19	263.0
20	449.0
21	777.0
22	1157.0
23	1652.0
24	2616.0
25	4071.0
26	5750.0
27	6888.0
28	7842.0
29	9052.0
30	10811.0
31	13815.0
32	18013.0
33	25086.0
34	44606.0
35	62169.0
36	67421.0
37	104764.0
38	198057.0
39	339540.0
40	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.956330046812393	3.770169743547627	56.7675892266662	17.505910982973777
2	18.63775075751354	3.9052470509922235	57.34885389606179	20.108148295432443
3	19.05022282352636	4.248343411901499	56.74230275471258	19.95913100985956
4	17.122723677214942	4.232134135008147	55.79460036568129	22.85054182209562
5	17.175025610657492	5.076205213767944	53.91010983406023	23.838659341514333
6	21.77035560992267	6.066375908259816	55.13888108442223	17.024387397395277
7	86.55019040497257	1.4674798680780985	8.471724537387237	3.5106051895620904
8	88.48125559220053	1.7354732460481783	6.620192868782662	3.1630782929686316
9	83.11328339435225	4.867321665535619	8.221561363999845	3.7978335761122803
10	36.84033646136356	36.84649598658304	14.199974929651738	12.113192622401654
11	25.63605202529512	25.28474296409321	30.137476280424814	18.94172873018686
12	24.10632853394655	22.864914047607726	32.39672529382016	20.632032124625567
13	23.270470155479384	22.929535031489223	33.72588599907499	20.074108813956403
14	20.152021404890448	25.82289095695248	32.03666322309584	21.98842441506123
15	17.475221418722363	25.995033477559875	35.09611020579298	21.43363489792478
16	20.280723063423657	24.78452467916438	34.54866889418152	20.386083363230444
17	20.492740405188698	24.459690770221613	31.62602820846427	23.421540616125423
18	20.510570609771385	23.973628587112977	33.335350487791175	22.180450315324464
19	22.091191230565077	24.704991160541002	32.21302015569551	20.990797453198415
20	24.99783876308089	25.142317451123624	31.148827096724	18.71101668907149
21	24.789927771462164	25.512861520905645	30.665358397918297	19.031852309713894
22	23.000855849819967	23.395605773096058	31.042926487687435	22.56061188939654
23	21.0666568690593	25.715369420226587	30.632399534901815	22.5855741758123
24	21.18768613652966	25.270370738581104	31.56745868795629	21.974484436932944
25	20.529049185429805	27.046367176862663	28.810909059472916	23.613674578234615
26	20.08372631824646	28.643521260087574	29.772659488478446	21.500092933187524
27	19.26688682466749	28.53286592982896	31.020989932958432	21.179257312545115
28	18.831613709158027	26.793070209942556	32.25786582176711	22.11745025913231
29	20.124033386787925	24.955154333928395	32.48468763642808	22.4361246428556
30	21.973079632935523	25.292199231464153	31.79795460537975	20.936766530220577
31	21.7576043120999	27.386329744239223	29.230405145472858	21.62566079818802
32	21.7251857583132	28.10386040138492	29.542920003976676	20.628033836325205
33	22.101997415160646	27.28096944443244	29.26412044141103	21.35291269899589
34	20.594858849616813	26.097908354909684	30.743595174390208	22.5636376210833
35	20.76267889638598	26.114117631803037	30.300649667817886	22.822553803993102
36	21.12511832772132	27.294261051484987	29.68826318678706	21.89235743400663
37	20.2511141176318	28.17561346709949	30.53481968800384	21.03845272726487
38	21.22194174169761	27.280537197048616	28.663728825281286	22.833792235972492
39	23.04926755680811	25.752326571543428	29.079550808518732	22.11885506312973
40	21.980211714768597	25.983903107426443	31.512455208364855	20.52342996944011
41	19.184867883587135	27.56538822298778	30.751267565453063	22.498476327972025
42	21.53564528050694	27.360827148593682	28.385685695637324	22.71784187526205
43	22.351728341164215	25.68619272181855	28.85002744770887	23.11205148930836
44	21.19341341436531	26.66815071601779	29.120074000752112	23.01836186886479
45	21.368365542967553	25.737414036801542	29.362240597538786	23.531979822692122
46	21.60210331576968	25.89442789897514	30.328745747766362	22.174723037488818
47	19.95696977294045	27.484017652983155	30.9671751336725	21.59183744040389
48	20.715023622319524	25.92241591707766	31.01256110897389	22.349999351628924
49	21.879281950645993	24.96520408560227	30.725656907961564	22.42985705579017
50	21.043531634024784	25.146099615732076	30.033520784615455	23.776847965627688
51	20.128031675088287	25.47449956559138	28.866128662756267	25.531340096564065
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	664.0
1	1649.0
2	2634.0
3	3925.5
4	5217.0
5	3716.5
6	2216.0
7	2355.0
8	2494.0
9	2770.0
10	3046.0
11	3193.5
12	3341.0
13	3561.0
14	3781.0
15	3753.5
16	3726.0
17	3746.0
18	3766.0
19	4059.5
20	4353.0
21	4954.5
22	5556.0
23	6168.0
24	6780.0
25	7411.5
26	10319.0
27	12595.0
28	14446.5
29	16298.0
30	19054.0
31	21810.0
32	24343.0
33	26876.0
34	30262.0
35	33648.0
36	35797.0
37	37946.0
38	41788.0
39	45630.0
40	48796.5
41	51963.0
42	55734.5
43	59506.0
44	63003.5
45	66501.0
46	70473.5
47	74446.0
48	79517.0
49	84588.0
50	80773.0
51	76958.0
52	67985.0
53	59012.0
54	51709.0
55	44406.0
56	40529.5
57	36653.0
58	34636.5
59	32620.0
60	29176.0
61	25732.0
62	23677.0
63	21622.0
64	18365.5
65	15109.0
66	12154.0
67	9199.0
68	7618.5
69	6038.0
70	4764.5
71	3491.0
72	3232.0
73	2973.0
74	2560.0
75	1500.0
76	853.0
77	729.0
78	605.0
79	481.5
80	358.0
81	242.0
82	126.0
83	88.5
84	51.0
85	29.0
86	7.0
87	6.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	925396.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.391799664400303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78259565091965	19.662544794606433
2	10.827575769758308	5.931735726726689
3	3.870419534488364	3.1805326951766015
4	1.9653644370841987	2.1533947571258896
5	1.2262765676788367	1.6794961037503553
6	0.8454337498345985	1.3894771142995224
7	0.6194149781385109	1.1876823693209289
8	0.5187682705039465	1.1367997230313218
9	0.47752040286463854	1.177212888983871
>10	7.194514608682784	46.168080269203074
>50	0.5837007675096606	10.177810606802383
>100	0.08201415755650322	3.802090286889443
>500	0.004000690612512351	0.7200903667718843
>1k	0.0024004143675074104	1.6330522973116146
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	3390	0.36632965778974624	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2727	0.29468465392113213	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	2609	0.28193335609836223	No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	2212	0.23903280325395831	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCC	2011	0.21731237221686717	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1953	0.21104478515143787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.38664528482941357	0.0
2	0.0	0.0	0.0	1.1751725747679913	0.0
3	0.0	0.0	0.0	1.605582907209454	0.0
4	0.0	0.0	0.0	2.0429091977920804	0.0
5	0.0	0.0	0.0	2.749741732188166	0.0
6	0.0	0.0	0.0	3.151299551759463	0.0
7	0.0	0.0	0.0	3.5114696843297355	0.0
8	0.0	0.0	0.0	4.130988247193634	0.0
9	0.0	0.0	0.0	4.431184055258505	0.0
10	0.0	0.0	0.0	5.075448780846254	0.0
11	0.0	0.0	0.0	6.45248088385945	0.0
12	0.0	0.0	0.0	7.590264059926778	0.0
13	0.0	0.0	0.0	7.9441666054316205	0.0
14	0.0	0.0	0.0	8.061954017523309	0.0
15	0.0	0.0	0.0	8.2606797522358	0.0
16	0.0	0.0	0.0	8.750307976260974	0.0
17	0.0	0.0	0.0	9.467838633406672	0.0
18	0.0	0.0	0.0	10.23983246091403	0.0
19	0.0	0.0	0.0	10.82606797522358	0.0
20	0.0	0.0	0.0	11.39728289294529	0.0
21	0.0	0.0	0.0	12.183648945964755	0.0
22	0.0	0.0	0.0	13.00870113983635	0.0
23	0.0	0.0	0.0	13.805333068221605	0.0
24	0.0	0.0	0.0	14.392865324682623	0.0
25	0.0	0.0	0.0	14.887248269929847	0.0
26	0.0	0.0	0.0	15.366178371205407	0.0
27	0.0	0.0	0.0	15.844568163251193	0.0
28	0.0	0.0	0.0	16.34683962325318	0.0
29	0.0	0.0	0.0	16.89676635732162	0.0
30	0.0	0.0	0.0	17.491214571923802	0.0
31	0.0	0.0	0.0	18.052379737971634	0.0
32	0.0	0.0	0.0	18.588582617603706	0.0
33	0.0	0.0	0.0	19.085342923461955	0.0
34	0.0	0.0	0.0	19.580374239784913	0.0
35	1.0806184595567736E-4	0.0	0.0	20.109769223121777	0.0
36	1.0806184595567736E-4	0.0	0.0	20.634841732620412	0.0
37	1.0806184595567736E-4	0.0	0.0	21.172557478095865	0.0
38	1.0806184595567736E-4	0.0	0.0	21.67266770117874	0.0
39	1.0806184595567736E-4	0.0	0.0	22.201306251593913	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCGTA	25	3.8902137E-5	45.000004	19
GTCGAGT	20	7.032573E-4	45.0	31
CGAGTAA	20	7.032573E-4	45.0	15
TAATTCG	20	7.032573E-4	45.0	22
CGCAGTA	35	1.2115925E-7	45.0	44
AACTCGT	20	7.032573E-4	45.0	34
GCGAGAC	105	0.0	42.857143	21
TCGGCGT	70	0.0	41.785713	4
TTCTACG	65	0.0	41.538464	1
TATTCCG	65	0.0	41.538464	1
CGGGTAC	45	1.927583E-8	40.0	6
TCGTTTG	310	0.0	39.919357	1
TTTGGGA	7630	0.0	39.57405	4
TCTAGCG	40	3.457244E-7	39.375	1
CGGGCTC	240	0.0	39.374996	6
ACGACCA	110	0.0	38.863632	28
TTGGGAT	3805	0.0	38.6728	5
TCGTTCA	100	0.0	38.250004	16
TCGCTTG	95	0.0	37.894737	1
TTGGGAC	2940	0.0	37.882652	5
>>END_MODULE