SRR3549731_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549731_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481900
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1546	0.32081344677318946	No Hit
CTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGCT	1277	0.26499273708238225	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	1262	0.2618800581033409	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCC	1201	0.24922183025523967	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	977	0.20273915750155633	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	894	0.18551566715086118	Illumina Single End Adapter 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCG	30	2.1628675E-6	45.000004	36
CTCGTAG	30	2.1628675E-6	45.000004	1
TACGGGA	85	0.0	45.000004	4
TCATCGA	35	1.2099554E-7	45.000004	16
TGTTTCG	45	3.8380676E-10	45.0	1
GATCGGT	25	3.8874234E-5	45.0	9
CCGTGAC	20	7.029206E-4	45.0	24
TAACGAA	20	7.029206E-4	45.0	22
TTATCCG	20	7.029206E-4	45.0	1
TATTGCG	25	3.8874234E-5	45.0	1
AGCGCAC	20	7.029206E-4	45.0	17
ACTACCG	20	7.029206E-4	45.0	39
ACCGTGT	20	7.029206E-4	45.0	42
ACGTAAT	20	7.029206E-4	45.0	38
TCCCCGT	20	7.029206E-4	45.0	21
ATCGGAA	20	7.029206E-4	45.0	15
CGGTAGT	55	1.8189894E-12	45.0	12
GCGCTTA	25	3.8874234E-5	45.0	37
AGGGCGC	20	7.029206E-4	45.0	6
TCGACAA	40	6.8012014E-9	45.0	19