FastQCFastQC Report
Sat 18 Jun 2016
SRR3549730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386720
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT51980.37484135225568244No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC48490.34967405099803855No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC42960.3097957770854967No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35890.258812161070728No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC26050.18785335179416177No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC24720.17826237452405677No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG17350.12511538017768548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA1150.045.0000048
CGCGTAT351.2121745E-745.026
CGTTCAT3000.039.75000417
TTTGGGA100050.038.815594
TTGGGAT49800.038.765065
TTTGGGC34650.038.441564
GCAACCC2000.038.24999622
CGCCGTT659.094947E-1238.0769226
TGGGATC21300.037.7112666
TTGGGAC41000.037.591465
GGCCGAT4850.037.577328
TCGCACC301.14004346E-437.50000441
CGACGTC301.14004346E-437.50000418
TTGGGAG47200.037.468225
CAACCCG2050.037.31707423
TCGTTCA3200.037.26562516
TGGGACG4050.037.222226
GCGCGAC7500.037.29
TATTGCG1150.037.1739161
CTTGTTG8300.037.1385541