##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1386720
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.506025008653516	33.0	31.0	34.0	31.0	34.0
2	32.66214881158417	34.0	31.0	34.0	31.0	34.0
3	32.68204107534326	34.0	31.0	34.0	31.0	34.0
4	36.096726808584286	37.0	35.0	37.0	35.0	37.0
5	36.105836794738664	37.0	35.0	37.0	35.0	37.0
6	35.92577304719049	37.0	35.0	37.0	35.0	37.0
7	36.021385715934	37.0	35.0	37.0	35.0	37.0
8	36.002557113187954	37.0	35.0	37.0	35.0	37.0
9	37.882164387908155	39.0	38.0	39.0	35.0	39.0
10	37.48660940925349	39.0	37.0	39.0	35.0	39.0
11	37.398794998269295	39.0	37.0	39.0	35.0	39.0
12	37.2625072112611	39.0	37.0	39.0	34.0	39.0
13	37.24924570208838	39.0	37.0	39.0	34.0	39.0
14	38.42405388254298	40.0	38.0	41.0	34.0	41.0
15	38.504394542517595	40.0	38.0	41.0	34.0	41.0
16	38.567421685704396	40.0	38.0	41.0	34.0	41.0
17	38.56405258451598	40.0	38.0	41.0	34.0	41.0
18	38.56244952117226	40.0	38.0	41.0	34.0	41.0
19	38.547930368062765	40.0	38.0	41.0	34.0	41.0
20	38.47875562478366	40.0	38.0	41.0	34.0	41.0
21	38.40820425175955	40.0	38.0	41.0	34.0	41.0
22	38.31500158647744	40.0	37.0	41.0	34.0	41.0
23	38.11213583131418	40.0	37.0	41.0	34.0	41.0
24	37.812031989154264	40.0	36.0	41.0	33.0	41.0
25	37.721979923849084	40.0	36.0	41.0	33.0	41.0
26	37.844481943002194	40.0	37.0	41.0	33.0	41.0
27	37.93303046036691	40.0	37.0	41.0	34.0	41.0
28	37.90622836621668	40.0	37.0	41.0	34.0	41.0
29	37.89828371985693	40.0	37.0	41.0	34.0	41.0
30	37.8380408445829	40.0	36.0	41.0	33.0	41.0
31	37.74326035537095	40.0	36.0	41.0	33.0	41.0
32	37.649749048113534	40.0	36.0	41.0	33.0	41.0
33	37.5089953271028	40.0	36.0	41.0	33.0	41.0
34	37.399289690781124	40.0	36.0	41.0	33.0	41.0
35	37.356104332525675	40.0	36.0	41.0	33.0	41.0
36	37.30911214953271	40.0	36.0	41.0	33.0	41.0
37	37.29655878620053	40.0	35.0	41.0	33.0	41.0
38	37.20403614284066	40.0	35.0	41.0	32.0	41.0
39	37.14708881389177	39.0	35.0	41.0	32.0	41.0
40	37.06962905272874	39.0	35.0	41.0	32.0	41.0
41	37.00458996769355	39.0	35.0	41.0	32.0	41.0
42	36.94539921541479	39.0	35.0	41.0	31.0	41.0
43	36.83413161993769	39.0	35.0	41.0	31.0	41.0
44	36.73235548632745	39.0	35.0	41.0	31.0	41.0
45	36.68821607822776	39.0	35.0	41.0	31.0	41.0
46	36.594375937463944	39.0	35.0	41.0	31.0	41.0
47	36.51683541017653	39.0	35.0	41.0	31.0	41.0
48	36.46083059305411	39.0	35.0	41.0	31.0	41.0
49	36.44167892581054	39.0	35.0	41.0	31.0	41.0
50	36.32752033575632	39.0	35.0	40.0	31.0	41.0
51	35.52600164416753	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	12.0
11	13.0
12	12.0
13	21.0
14	27.0
15	47.0
16	97.0
17	128.0
18	229.0
19	412.0
20	821.0
21	1300.0
22	1953.0
23	2925.0
24	4265.0
25	6419.0
26	8713.0
27	10828.0
28	12684.0
29	15247.0
30	18730.0
31	23631.0
32	30861.0
33	42405.0
34	74578.0
35	99868.0
36	106009.0
37	163716.0
38	297385.0
39	463009.0
40	363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.15322487596631	4.68443521403023	55.72062132225683	18.441718587746625
2	20.08386696665513	4.393028152763355	54.57691531094958	20.946189569631937
3	19.463914849428868	4.1767624322141454	53.686757240106154	22.672565478250835
4	18.36470231914157	4.4690348448136605	52.944934810199605	24.22132802584516
5	17.216741663782162	5.511927425868236	52.178882542979125	25.092448367370483
6	21.449102919118495	6.331703588323526	55.250663436021696	16.968530056536288
7	84.08164589823468	2.052036460136149	9.477544132917965	4.388773508711203
8	85.83052094150226	2.4367572401061497	7.415267682012231	4.31745413637937
9	80.15107592015693	5.1636235144802125	9.689627322026077	4.995673243336795
10	36.175074997115495	32.29044075227876	16.636090919580017	14.89839333102573
11	27.103885427483558	24.443002192223375	27.824074074074073	20.62903830621899
12	25.086823583708316	21.961751471097266	31.76639840775355	21.18502653744087
13	23.40047017422407	22.727803738317757	33.19617514710973	20.675550940348447
14	20.72848159686166	25.121221299180803	32.23138052382601	21.918916580131533
15	18.521619360793814	24.810127495096342	34.27440290758047	22.393850236529364
16	20.435776508595822	23.88723029883466	32.98127956617053	22.695713626398984
17	20.255783431406485	24.16709934233299	30.652907580477674	24.924209645782856
18	21.289229260413062	23.51707626629745	32.18429098880812	23.009403484481368
19	22.16085439021576	24.89731164185993	30.592693550248068	22.349140417676246
20	24.36930310372678	25.839246567439716	29.796570324218298	19.994880004615208
21	24.1884446752048	25.543945425175956	29.707006461289954	20.560603438329295
22	23.266268605053654	23.623442367601246	28.94802122995269	24.16226779739241
23	22.04850294219453	24.44624725972078	28.93143532941041	24.573814468674282
24	21.955261336102456	24.50004326756663	30.59103496019384	22.95366043613707
25	21.48761105342102	25.76987423560632	27.825732664128306	24.91678204684435
26	20.129153686396677	26.393648321218414	28.487365870543442	24.989832121841467
27	19.69157436252452	25.472914503288337	30.587645667474327	24.247865466712817
28	18.752019153109494	25.228813314872507	31.41434464059075	24.604822891427254
29	19.703689281181493	23.382514134071766	31.35975539402331	25.55404119072343
30	21.541190723433715	23.79831544940579	30.73086131302642	23.929632514134074
31	21.10512576439368	26.08745817468559	28.461116880119995	24.346299180800738
32	20.392653167185877	26.2246163609092	28.189180223837546	25.193550248067382
33	20.762951424945193	25.357390100380755	28.331530518056997	25.54812795661705
34	19.315218645436712	24.58542459905388	29.696045344409832	26.403311411099573
35	19.222265489788853	24.34363101419176	29.49196665512865	26.942136840890736
36	19.551892234914042	26.24754817122418	29.01407638167763	25.186483212184147
37	18.95934290988808	26.64460020768432	30.856120918426218	23.539935964001383
38	19.310603438329295	26.29968558901581	29.648090458059308	24.741620514595592
39	19.645782854505594	25.867082035306332	29.803204684435215	24.683930425752855
40	19.901566285912082	24.08431406484366	31.486673589477327	24.527446059766934
41	18.17576727818161	25.065838813891773	29.775441329179646	26.98295257874697
42	19.515691704165224	26.12683166032076	28.088655244029077	26.26882139148494
43	20.09655878620053	24.661863966770508	29.62011076497058	25.62146648205838
44	19.972092419522326	23.933454482519902	29.28377754701742	26.81067555094035
45	20.105284412137994	23.209660205376718	29.638138917733936	27.04691646475136
46	20.376355717087804	23.576857620860736	30.819631937233183	25.227154724818273
47	18.10617860851506	24.690059997692394	31.648854851736473	25.554906542056077
48	17.79169551171109	23.85939483096804	32.01078804661359	26.338121610707283
49	19.39800392292604	23.478784469828085	31.85365466712819	25.26955694011769
50	19.651479750778815	22.32043959847698	30.95001153801777	27.078069112726432
51	18.547291450328835	22.97781816083997	29.75654782508365	28.71834256374755
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	806.0
1	2199.0
2	3592.0
3	5153.0
4	6714.0
5	4671.0
6	2628.0
7	2871.0
8	3114.0
9	3461.0
10	3808.0
11	3974.0
12	4140.0
13	4051.5
14	3963.0
15	3884.0
16	3805.0
17	4098.5
18	4392.0
19	4679.5
20	4967.0
21	5299.5
22	5632.0
23	6845.0
24	8058.0
25	8878.5
26	12570.0
27	15441.0
28	17883.5
29	20326.0
30	23956.5
31	27587.0
32	31252.0
33	34917.0
34	38350.0
35	41783.0
36	44838.5
37	47894.0
38	53153.5
39	58413.0
40	63257.0
41	68101.0
42	74599.0
43	81097.0
44	86579.5
45	92062.0
46	102472.0
47	112882.0
48	126784.5
49	140687.0
50	134216.0
51	127745.0
52	111230.0
53	94715.0
54	84153.0
55	73591.0
56	67119.0
57	60647.0
58	57097.5
59	53548.0
60	49126.0
61	44704.0
62	40643.5
63	36583.0
64	32703.5
65	28824.0
66	23994.0
67	19164.0
68	16382.0
69	13600.0
70	11766.0
71	9932.0
72	8512.5
73	7093.0
74	6359.0
75	3802.0
76	1979.0
77	1594.5
78	1210.0
79	913.5
80	617.0
81	469.5
82	322.0
83	235.5
84	149.0
85	132.5
86	116.0
87	71.5
88	27.0
89	17.5
90	8.0
91	7.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1386720.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.03523267585508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8383498203109	19.42166502441577
2	11.063137319500733	5.981889831152759
3	4.149002027282506	3.365077055405309
4	2.0133780623837496	2.1772857752402777
5	1.1877853534370617	1.605602669957186
6	0.8279950536319102	1.3431023357637477
7	0.6377616092414757	1.2069423448299743
8	0.5037563218278975	1.0895335498040115
9	0.427983271381861	1.0413564590864015
>10	6.571696262760065	43.98658552279633
>50	0.6869043660108738	11.550438099118878
>100	0.08359359080460196	4.056979177870683
>500	0.005410588391499422	0.9746404266241648
>1k	0.002975823615324682	1.81872906264919
>5k	2.705294195749711E-4	0.38017266528533766
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	5198	0.37484135225568244	No Hit
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	4849	0.34967405099803855	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	4296	0.3097957770854967	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3589	0.258812161070728	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2605	0.18785335179416177	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	2472	0.17826237452405677	No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG	1735	0.12511538017768548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4422522210684206E-4	0.0	0.0	0.3972683742932964	0.0
2	1.4422522210684206E-4	0.0	0.0	1.3237712011076497	0.0
3	1.4422522210684206E-4	0.0	0.0	1.9137244721356872	0.0
4	1.4422522210684206E-4	0.0	0.0	2.584083304488289	0.0
5	1.4422522210684206E-4	0.0	0.0	3.6027460482289144	0.0
6	1.4422522210684206E-4	0.0	0.0	4.279018114687896	0.0
7	1.4422522210684206E-4	0.0	0.0	4.871711664935964	0.0
8	1.4422522210684206E-4	0.0	0.0	5.819055036344756	0.0
9	2.1633783316026307E-4	0.0	0.0	6.242644513672551	0.0
10	2.1633783316026307E-4	0.0	0.0	7.069415599400023	0.0
11	2.1633783316026307E-4	0.0	0.0	8.732043959847697	0.0
12	2.1633783316026307E-4	0.0	0.0	10.147614514826353	0.0
13	2.1633783316026307E-4	0.0	0.0	10.583318910811123	0.0
14	2.1633783316026307E-4	0.0	0.0	10.738577362409139	0.0
15	2.1633783316026307E-4	0.0	0.0	10.978640244605977	0.0
16	2.1633783316026307E-4	0.0	0.0	11.514148494288682	0.0
17	2.1633783316026307E-4	0.0	0.0	12.281787238952347	0.0
18	2.1633783316026307E-4	0.0	0.0	13.1524027922003	0.0
19	2.1633783316026307E-4	0.0	0.0	13.768532941040728	0.0
20	2.1633783316026307E-4	0.0	0.0	14.405864197530864	0.0
21	2.1633783316026307E-4	0.0	0.0	15.289315795546326	0.0
22	2.1633783316026307E-4	0.0	0.0	16.22750086535133	0.0
23	2.1633783316026307E-4	0.0	0.0	17.071002076843197	0.0
24	2.1633783316026307E-4	0.0	0.0	17.78015749394254	0.0
25	2.1633783316026307E-4	0.0	0.0	18.3484048690435	0.0
26	2.1633783316026307E-4	0.0	0.0	18.875475943232953	0.0
27	2.884504442136841E-4	0.0	0.0	19.3866101303796	0.0
28	2.884504442136841E-4	0.0	0.0	19.932502596053997	0.0
29	2.884504442136841E-4	0.0	0.0	20.51286488981193	0.0
30	2.884504442136841E-4	0.0	0.0	21.14969135802469	0.0
31	2.884504442136841E-4	0.0	0.0	21.714693665628246	0.0
32	4.3267566632052614E-4	0.0	0.0	22.272917387792777	0.0
33	5.047882773739472E-4	0.0	0.0	22.81448309680397	0.0
34	5.047882773739472E-4	0.0	0.0	23.352082612207223	0.0
35	5.047882773739472E-4	0.0	0.0	23.91484942886812	0.0
36	5.047882773739472E-4	0.0	0.0	24.45360274604823	0.0
37	5.047882773739472E-4	0.0	0.0	24.98355832467982	0.0
38	5.047882773739472E-4	0.0	0.0	25.504355601707626	0.0
39	5.047882773739472E-4	0.0	0.0	26.053565247490482	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGATA	115	0.0	45.000004	8
CGCGTAT	35	1.2121745E-7	45.0	26
CGTTCAT	300	0.0	39.750004	17
TTTGGGA	10005	0.0	38.81559	4
TTGGGAT	4980	0.0	38.76506	5
TTTGGGC	3465	0.0	38.44156	4
GCAACCC	200	0.0	38.249996	22
CGCCGTT	65	9.094947E-12	38.07692	26
TGGGATC	2130	0.0	37.711266	6
TTGGGAC	4100	0.0	37.59146	5
GGCCGAT	485	0.0	37.57732	8
TCGCACC	30	1.14004346E-4	37.500004	41
CGACGTC	30	1.14004346E-4	37.500004	18
TTGGGAG	4720	0.0	37.46822	5
CAACCCG	205	0.0	37.317074	23
TCGTTCA	320	0.0	37.265625	16
TGGGACG	405	0.0	37.22222	6
GCGCGAC	750	0.0	37.2	9
TATTGCG	115	0.0	37.173916	1
CTTGTTG	830	0.0	37.138554	1
>>END_MODULE