SRR3549728_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187056
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	5120	0.43131916270167536	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3841	0.3235736140502217	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3841	0.3235736140502217	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3655	0.30790459759269995	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3611	0.3041979485382324	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	1706	0.14371689288458167	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1705	0.14363265086061652	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	1446	0.12181396665363724	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	55	1.8189894E-12	45.0	2
GCGATAT	20	7.03338E-4	45.0	9
TTAACGA	25	3.890883E-5	45.0	16
ACTCGAC	20	7.03338E-4	45.0	23
TCGTAGC	20	7.03338E-4	45.0	15
TTACGCC	20	7.03338E-4	45.0	31
CGCAAAT	20	7.03338E-4	45.0	40
TCGACCA	20	7.03338E-4	45.0	25
GTGCGAT	20	7.03338E-4	45.0	43
CGATAGG	20	7.03338E-4	45.0	10
CGTTTTT	2105	0.0	42.114014	1
GTTAGCG	50	1.0822987E-9	40.5	1
CCGTCGG	50	1.0822987E-9	40.5	2
TAGCGGG	220	0.0	39.886364	3
TTCGTAA	40	3.4583536E-7	39.375	16
TAACGGA	40	3.4583536E-7	39.375	38
TTTCGCG	370	0.0	38.918915	1
CGTTGAT	145	0.0	38.793102	25
CTTCGTA	35	6.2484614E-6	38.57143	15
CGAGACT	35	6.2484614E-6	38.57143	26