##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187056
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10513236106805	33.0	31.0	34.0	30.0	34.0
2	32.28588878704964	34.0	31.0	34.0	30.0	34.0
3	32.36656231887965	34.0	31.0	34.0	30.0	34.0
4	35.88990831098111	37.0	35.0	37.0	35.0	37.0
5	35.86512851963176	37.0	35.0	37.0	35.0	37.0
6	35.503131276030786	37.0	35.0	37.0	33.0	37.0
7	35.862916324082434	37.0	35.0	37.0	35.0	37.0
8	35.934655146850695	37.0	35.0	37.0	35.0	37.0
9	37.72010503295548	39.0	38.0	39.0	35.0	39.0
10	37.14472274265073	39.0	37.0	39.0	34.0	39.0
11	37.05441023843863	39.0	37.0	39.0	33.0	39.0
12	36.95107981426319	39.0	37.0	39.0	33.0	39.0
13	36.879243270747125	39.0	37.0	39.0	33.0	39.0
14	38.04747880470677	40.0	37.0	41.0	33.0	41.0
15	38.08410133978515	40.0	37.0	41.0	33.0	41.0
16	38.12185271798466	40.0	37.0	41.0	33.0	41.0
17	38.042756196843285	40.0	37.0	41.0	33.0	41.0
18	38.024751991481445	40.0	37.0	41.0	33.0	41.0
19	38.04413271151488	40.0	37.0	41.0	34.0	41.0
20	37.99387813211845	40.0	37.0	41.0	33.0	41.0
21	37.842934958418134	40.0	36.0	41.0	33.0	41.0
22	37.922124988206114	40.0	37.0	41.0	33.0	41.0
23	37.84693308487552	40.0	36.0	41.0	33.0	41.0
24	37.80014590718551	40.0	36.0	41.0	33.0	41.0
25	37.71179034519012	39.0	36.0	41.0	33.0	41.0
26	37.63300383469693	39.0	36.0	41.0	33.0	41.0
27	37.556752166704854	39.0	36.0	41.0	33.0	41.0
28	37.4436420859673	39.0	36.0	41.0	32.0	41.0
29	37.36105962987424	39.0	36.0	41.0	32.0	41.0
30	37.35962161852516	39.0	36.0	41.0	32.0	41.0
31	37.31704485719292	39.0	36.0	41.0	32.0	41.0
32	37.18233343667021	39.0	35.0	41.0	32.0	41.0
33	37.115430948497796	39.0	35.0	41.0	32.0	41.0
34	37.00039762235311	39.0	35.0	41.0	31.0	41.0
35	36.904473756924695	39.0	35.0	41.0	31.0	41.0
36	36.732599809949996	39.0	35.0	41.0	31.0	41.0
37	36.71504545699613	39.0	35.0	41.0	31.0	41.0
38	36.65218911323476	39.0	35.0	41.0	30.0	41.0
39	36.60608766561982	39.0	35.0	41.0	30.0	41.0
40	36.4845651763691	39.0	35.0	40.0	30.0	41.0
41	36.38238549824103	39.0	35.0	40.0	30.0	41.0
42	36.3111487579356	39.0	35.0	40.0	30.0	41.0
43	36.22453110889461	39.0	35.0	40.0	30.0	41.0
44	36.072877774932266	38.0	35.0	40.0	30.0	41.0
45	36.106864377080775	38.0	35.0	40.0	30.0	41.0
46	35.98933327492553	38.0	35.0	40.0	29.0	41.0
47	35.78473130164036	38.0	35.0	40.0	29.0	41.0
48	35.68993543691283	38.0	35.0	40.0	28.0	41.0
49	35.687109959429044	38.0	35.0	40.0	28.0	41.0
50	35.591726927794475	38.0	34.0	40.0	28.0	41.0
51	34.630496792063724	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	29.0
11	18.0
12	13.0
13	16.0
14	22.0
15	32.0
16	71.0
17	110.0
18	231.0
19	419.0
20	765.0
21	1363.0
22	1990.0
23	3058.0
24	4674.0
25	6736.0
26	9400.0
27	11328.0
28	13424.0
29	16149.0
30	20051.0
31	26044.0
32	33682.0
33	46046.0
34	73740.0
35	92869.0
36	105994.0
37	157381.0
38	262137.0
39	299152.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.782692644660404	4.77921850359208	53.57413635077031	15.863952500977208
2	19.587028750117938	4.87795015567926	53.199259344125295	22.335761750077502
3	19.90605329487404	4.864724157916728	53.15140987451308	22.077812672696147
4	18.02686646628297	4.944332870563815	52.03006429351269	24.998736369640522
5	18.0403451901174	5.644384089714386	51.88592619050828	24.429344529659932
6	21.367989378765618	6.481918291976116	54.35160598994487	17.798486339313392
7	83.09085670768691	2.018523136229462	10.241724063565663	4.64889609251796
8	84.68286247658072	2.9903391246916744	7.718675445808791	4.6081229529188175
9	79.37056044533703	4.925631141243548	10.068690946341201	5.635117467078217
10	37.40548044911108	25.83298513296761	19.725943847636508	17.035590570284807
11	27.579322289765607	27.034866088878708	25.09157108005014	20.294240541305548
12	25.503683061287756	22.478467738674503	30.983710962246096	21.034138237791648
13	23.352731463385044	24.321177770888653	31.69193365772129	20.634157108005013
14	20.203848849590923	26.31703980267148	30.61708967394967	22.862021673787925
15	18.16940397083204	26.639939480529982	34.25011119947163	20.94054534916634
16	20.11345715787629	24.47786793546387	32.83324459840142	22.575430308258415
17	19.43674097936407	26.09733660417032	30.151736733566064	24.31418568289954
18	20.5280121578089	25.435868231995794	30.95287838147484	23.083241228720464
19	22.344522920569883	28.482059818576378	28.140205685325714	21.03321157552803
20	25.363757059481607	25.66677561968433	29.500377404267365	19.469089916566702
21	24.02287676402798	27.556492701271047	28.565122454206037	19.85550808049494
22	22.14983960318637	25.450863312261596	27.90584437465461	24.493452709897426
23	21.52948133870685	27.27461888908358	27.479579733390842	23.716320038818726
24	21.936960008626382	25.49904974996967	29.42228504805165	23.141705193352294
25	20.929172675931042	27.822613254977018	27.006223800730545	24.24199026836139
26	19.629992182340175	30.14533433974471	27.905338922510815	22.319334555404293
27	20.51369101373482	28.77353722149587	29.1790783248642	21.53369343990511
28	18.19231780135057	28.171038266096964	31.38731449906323	22.249329433489237
29	19.72863959240339	26.646341874351336	29.446967961073444	24.178050572171827
30	21.252577805933335	27.30435632354329	28.75963728754162	22.68342858298176
31	19.878421911013465	28.963755711609224	27.478821555175152	23.679000822202152
32	20.27924546103975	29.278483913143106	26.779949724360097	23.66232090145705
33	22.104517394293108	28.0807308164063	26.50043468884366	23.31431710045693
34	19.983471714898034	27.66280613551509	27.711919235486782	24.641802914100094
35	19.73596864848836	27.324827135366824	27.105713631033414	25.833490585111402
36	20.810138696068258	30.331256486635844	26.2035657963904	22.6550390209055
37	19.04813252281274	29.075039425267217	29.25447493631303	22.622353115607012
38	19.73672682670405	26.519726112331682	29.034350527692038	24.70919653327223
39	22.30930975455244	24.804811230472698	28.015948699977084	24.869930314997777
40	22.205692065075276	25.081209311102427	29.020450593737785	23.692648030084513
41	18.707036567777763	26.79755630736882	29.306115297003682	25.18929182784974
42	21.1305111132078	27.192230189645645	27.76204323974606	23.915215457400492
43	21.321487781536845	26.853156043185834	28.26463115472227	23.560725020555054
44	20.56844832931218	26.743388685959214	26.899741882438573	25.788421102290037
45	20.960763434917983	25.801731342076533	26.319314337318545	26.918190885686943
46	20.684702322384116	27.380258387135907	27.59726584087019	24.33777344960979
47	18.880069685002223	27.617568168645796	29.82664676308447	23.675715383267512
48	19.43733067353183	25.340000808723428	30.264958013775257	24.957710503969484
49	20.794806647706597	24.59403768651184	30.008356808777343	24.602798857004217
50	20.23527112452993	24.03980941084498	28.533531695219093	27.191387769405996
51	19.165144694100363	23.702251620816543	27.260381987033465	29.87222169804963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	252.0
1	875.5
2	1499.0
3	3925.0
4	6351.0
5	4509.0
6	2667.0
7	2707.5
8	2748.0
9	2885.0
10	3022.0
11	3175.5
12	3329.0
13	3319.5
14	3310.0
15	3489.5
16	3669.0
17	3486.0
18	3303.0
19	3718.0
20	4133.0
21	4378.5
22	4624.0
23	5301.0
24	5978.0
25	6337.5
26	9518.0
27	12339.0
28	13569.5
29	14800.0
30	16777.0
31	18754.0
32	22598.5
33	26443.0
34	31729.0
35	37015.0
36	39744.5
37	42474.0
38	46285.5
39	50097.0
40	54655.0
41	59213.0
42	67001.0
43	74789.0
44	82377.0
45	89965.0
46	100137.0
47	110309.0
48	121161.0
49	132013.0
50	126365.0
51	120717.0
52	101080.5
53	81444.0
54	69840.5
55	58237.0
56	52817.5
57	47398.0
58	45225.0
59	43052.0
60	40679.5
61	38307.0
62	32977.5
63	27648.0
64	22454.0
65	17260.0
66	14339.0
67	11418.0
68	9462.0
69	7506.0
70	6171.5
71	4837.0
72	4264.0
73	3691.0
74	3326.0
75	2244.5
76	1528.0
77	1096.0
78	664.0
79	484.5
80	305.0
81	225.0
82	145.0
83	85.5
84	26.0
85	44.0
86	62.0
87	52.5
88	43.0
89	26.0
90	9.0
91	5.0
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1187056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.68677044994076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.82924905404195	23.478661754210943
2	12.117119798303474	7.921390267231564
3	4.75043805848286	4.658294350628746
4	2.4104164587460866	3.151549179031697
5	1.3965911504882094	2.282502717421339
6	0.8980273603007011	1.7612168510349155
7	0.6464191222081442	1.4790547423448872
8	0.512540126093088	1.3402625158390769
9	0.39738140324892285	1.1690203238165597
>10	4.421251898629166	33.843264120297064
>50	0.5342850754938232	11.002553967346646
>100	0.07954387882918602	4.64372580947949
>500	0.003368307567189497	0.6981129618598291
>1k	0.0031092069850979973	2.1367693834433448
>5k	2.591005820914998E-4	0.43362105601386935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	5120	0.43131916270167536	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3841	0.3235736140502217	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3841	0.3235736140502217	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	3655	0.30790459759269995	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3611	0.3041979485382324	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	1706	0.14371689288458167	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1705	0.14363265086061652	No Hit
TCCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	1446	0.12181396665363724	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6848404793034195E-4	0.0	0.0	0.46661657074308205	0.0
2	1.6848404793034195E-4	0.0	0.0	1.5314357536628431	0.0
3	1.6848404793034195E-4	0.0	0.0	2.168810906983327	0.0
4	1.6848404793034195E-4	0.0	0.0	2.8992734967853244	0.0
5	1.6848404793034195E-4	0.0	0.0	4.143949400870725	0.0
6	1.6848404793034195E-4	0.0	0.0	4.941131673653138	0.0
7	2.527260718955129E-4	0.0	0.0	5.63435928886253	0.0
8	2.527260718955129E-4	0.0	0.0	6.746606731274683	0.0
9	2.527260718955129E-4	0.0	0.0	7.250879486730196	0.0
10	2.527260718955129E-4	0.0	0.0	8.41316669137766	0.0
11	2.527260718955129E-4	0.0	0.0	10.5098664258468	0.0
12	2.527260718955129E-4	0.0	0.0	12.263869606825626	0.0
13	2.527260718955129E-4	0.0	0.0	12.875887910932592	0.0
14	2.527260718955129E-4	0.0	0.0	13.081606933455541	0.0
15	2.527260718955129E-4	0.0	0.0	13.424893181113612	0.0
16	2.527260718955129E-4	0.0	0.0	14.170603577253306	0.0
17	2.527260718955129E-4	0.0	0.0	15.290517043846288	0.0
18	2.527260718955129E-4	0.0	0.0	16.524157242792253	0.0
19	2.527260718955129E-4	0.0	0.0	17.384689517596474	0.0
20	3.369680958606839E-4	0.0	0.0	18.253814478845143	0.0
21	3.369680958606839E-4	0.0	0.0	19.539853216697445	0.0
22	3.369680958606839E-4	0.0	0.0	20.8528494022186	0.0
23	3.369680958606839E-4	0.0	0.0	22.25413122885525	0.0
24	3.369680958606839E-4	0.0	0.0	23.22510479707781	0.0
25	3.369680958606839E-4	0.0	0.0	24.09195522367942	0.0
26	3.369680958606839E-4	0.0	0.0	24.82502931622434	0.0
27	3.369680958606839E-4	0.0	0.0	25.553301613403242	0.0
28	3.369680958606839E-4	0.0	0.0	26.371544392176947	0.0
29	3.369680958606839E-4	0.0	0.0	27.17765631949967	0.0
30	3.369680958606839E-4	0.0	0.0	28.056469113504335	0.0
31	3.369680958606839E-4	0.0	0.0	28.916664420212694	0.0
32	3.369680958606839E-4	0.0	0.0	29.707444299173755	0.0
33	3.369680958606839E-4	0.0	0.0	30.525602835923493	0.0
34	3.369680958606839E-4	0.0	0.0	31.228602525912848	0.0
35	3.369680958606839E-4	0.0	0.0	31.997816446738824	0.0
36	3.369680958606839E-4	0.0	0.0	32.76652491542101	0.0
37	3.369680958606839E-4	0.0	0.0	33.55292420913588	0.0
38	3.369680958606839E-4	0.0	0.0	34.305373967192786	0.0
39	3.369680958606839E-4	0.0	0.0	35.037858365569946	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	55	1.8189894E-12	45.0	2
GCGATAT	20	7.03338E-4	45.0	9
TTAACGA	25	3.890883E-5	45.0	16
ACTCGAC	20	7.03338E-4	45.0	23
TCGTAGC	20	7.03338E-4	45.0	15
TTACGCC	20	7.03338E-4	45.0	31
CGCAAAT	20	7.03338E-4	45.0	40
TCGACCA	20	7.03338E-4	45.0	25
GTGCGAT	20	7.03338E-4	45.0	43
CGATAGG	20	7.03338E-4	45.0	10
CGTTTTT	2105	0.0	42.114014	1
GTTAGCG	50	1.0822987E-9	40.5	1
CCGTCGG	50	1.0822987E-9	40.5	2
TAGCGGG	220	0.0	39.886364	3
TTCGTAA	40	3.4583536E-7	39.375	16
TAACGGA	40	3.4583536E-7	39.375	38
TTTCGCG	370	0.0	38.918915	1
CGTTGAT	145	0.0	38.793102	25
CTTCGTA	35	6.2484614E-6	38.57143	15
CGAGACT	35	6.2484614E-6	38.57143	26
>>END_MODULE