FastQCFastQC Report
Sat 18 Jun 2016
SRR3549727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2680027
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490561.8304293203016238No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT147140.5490243195311092No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT134070.5002561541357605No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT128280.47865189417867804No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC127470.47562953656810175No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC109740.40947348664770916No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC102020.38066780670493244No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC96290.3592874250893741No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG78140.2915642267783123No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC70800.264176442998522No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC60840.22701263830550963No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT60540.22589324659788873No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGT58200.2171619912784461No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG46150.17219975768900836No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC45710.17055798318449777No Hit
TCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG45480.16969978287532178No Hit
TTCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT43240.16134165812508605No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCGA207.0349703E-445.012
CGCGGAA351.2127384E-745.04
GTACGAG253.8922022E-545.01
CGTTTTT312050.044.5096931
TACGGGT603.6379788E-1241.2500044
CGTTTTC15000.041.2499961
CGTTTCT19350.041.0465131
TTCTCCG4200.040.7142871
TACTTCG451.9299478E-840.01
AGGGCGA4000.039.3756
CGTTCTG14650.039.1638221
GTTTTTT351450.039.033292
TTACGGG6150.038.7804873
TACGGGA3900.038.6538434
TTGGGAC69200.038.497115
CTACGGG2350.038.297873
TTGCTTG22200.038.108111
TTTCGCG9350.038.021391
GCGCGAC1600.037.968759
TCTACGG1600.037.968752
Produced by FastQC (version 0.11.5)