##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549727_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2680027
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.979605802478854	33.0	31.0	34.0	30.0	34.0
2	32.22923313832286	33.0	31.0	34.0	30.0	34.0
3	32.17670456305105	33.0	31.0	34.0	30.0	34.0
4	35.8273953956434	37.0	35.0	37.0	35.0	37.0
5	35.845100814282844	37.0	35.0	37.0	35.0	37.0
6	35.56717562920075	37.0	35.0	37.0	33.0	37.0
7	35.906290869457656	37.0	35.0	37.0	35.0	37.0
8	35.98652177757911	37.0	35.0	37.0	35.0	37.0
9	37.773263478315705	39.0	38.0	39.0	35.0	39.0
10	37.208110963061195	39.0	37.0	39.0	34.0	39.0
11	37.116126068879154	39.0	37.0	39.0	33.0	39.0
12	37.1063250482178	39.0	37.0	39.0	33.0	39.0
13	37.108626890699234	39.0	37.0	39.0	33.0	39.0
14	38.26835998294047	40.0	38.0	41.0	33.0	41.0
15	38.260755208809464	40.0	38.0	41.0	33.0	41.0
16	38.254839969895826	40.0	38.0	41.0	33.0	41.0
17	38.189487643221504	40.0	37.0	41.0	33.0	41.0
18	38.0022477385489	39.0	37.0	41.0	33.0	41.0
19	37.88113627213457	39.0	37.0	41.0	33.0	41.0
20	37.666961564193194	39.0	35.0	41.0	33.0	41.0
21	37.517379115956665	39.0	35.0	41.0	33.0	41.0
22	37.61180988101986	39.0	35.0	41.0	33.0	41.0
23	37.560747708885025	39.0	35.0	41.0	33.0	41.0
24	37.49427487111137	39.0	35.0	41.0	33.0	41.0
25	37.43226542120658	39.0	35.0	41.0	33.0	41.0
26	37.389604283837436	39.0	35.0	41.0	33.0	41.0
27	37.3316183008604	39.0	35.0	41.0	33.0	41.0
28	37.21094451660375	39.0	35.0	41.0	33.0	41.0
29	37.11060709463002	39.0	35.0	40.0	32.0	41.0
30	37.02586391853515	39.0	35.0	40.0	32.0	41.0
31	36.83476770942979	39.0	35.0	40.0	31.0	41.0
32	36.50114793619616	39.0	35.0	40.0	30.0	41.0
33	36.16217000798873	39.0	35.0	40.0	30.0	41.0
34	35.79916694869119	39.0	35.0	40.0	27.0	41.0
35	35.572898705871246	39.0	35.0	40.0	25.0	41.0
36	35.355813206359485	39.0	35.0	40.0	24.0	41.0
37	35.27655990032936	39.0	35.0	40.0	24.0	41.0
38	35.177513883255656	39.0	35.0	40.0	23.0	41.0
39	35.08624913107219	39.0	35.0	40.0	23.0	41.0
40	34.9988149373122	38.0	35.0	40.0	23.0	41.0
41	34.891264155174554	38.0	34.0	40.0	22.0	41.0
42	34.79808636256276	38.0	34.0	40.0	22.0	41.0
43	34.71713419305104	38.0	34.0	40.0	21.0	41.0
44	34.571682300215635	38.0	34.0	40.0	21.0	41.0
45	34.60884013481954	38.0	34.0	40.0	21.0	41.0
46	34.49195735714603	38.0	34.0	40.0	20.0	41.0
47	34.26610030421335	38.0	33.0	40.0	20.0	41.0
48	34.14991304192085	38.0	33.0	40.0	20.0	41.0
49	34.1476197814425	38.0	33.0	40.0	19.0	41.0
50	34.03740111573503	38.0	33.0	40.0	19.0	41.0
51	33.217011619659054	37.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	15.0
10	21.0
11	19.0
12	13.0
13	13.0
14	24.0
15	38.0
16	127.0
17	256.0
18	640.0
19	1430.0
20	2794.0
21	4698.0
22	7457.0
23	12343.0
24	21205.0
25	37451.0
26	53667.0
27	56029.0
28	51150.0
29	48677.0
30	52194.0
31	61283.0
32	76084.0
33	98650.0
34	146121.0
35	189088.0
36	236861.0
37	341374.0
38	571990.0
39	608186.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.121673027920988	3.9248112052602453	54.530719280066954	22.42279648675181
2	28.678890175360173	3.6814181349665507	48.03619515773535	19.603496531937925
3	16.058905376699563	3.633023100140409	59.33660369839557	20.971467824764453
4	15.147048891671613	3.4772410874965063	58.61138712408495	22.764322896746936
5	15.34003202206545	4.317866946862849	58.179861620797105	22.162239410274598
6	17.796499811382496	5.078269733849696	59.82585250073973	17.299377954028074
7	73.16885240335266	1.1552122422647235	20.214348586786627	5.461586767595998
8	75.07409440278026	1.3409566396159442	18.196122651003144	5.3888263066006425
9	69.9989589657119	3.717275982667339	20.265318222540294	6.018446829080453
10	30.65390759122949	27.786660358272506	27.105809008640584	14.453623041857414
11	18.829175974719657	23.15808758643103	37.79405953745989	20.218676901389426
12	17.53090547222099	20.13020764343046	41.34913566169296	20.989751222655592
13	18.758094601285734	20.634344355485972	42.767740772760874	17.839820270467424
14	16.97934386481927	23.319168053157675	40.94298303711119	18.75850504491186
15	14.696866859923427	25.194932737617943	41.38029206422174	18.72790833823689
16	16.30942524086511	25.297506331092933	39.072666058961346	19.32040236908061
17	16.349387524827176	25.95705192522314	36.70033921300047	20.993221336949215
18	16.50035615312831	25.404781369739933	38.19267492454367	19.902187552588092
19	16.84871085253992	27.772444083585725	36.36127546476211	19.017569599112246
20	17.69620231437967	27.96542721397956	36.090755802087074	18.247614669553702
21	18.133959098173264	27.919308275625582	36.56231821545081	17.38441441075034
22	17.017515122049144	25.177283661694457	36.0538531887925	21.7513480274639
23	16.277970333880965	25.88600786484614	35.320838185585444	22.515183615687455
24	17.33116867852451	25.421012549500432	35.69490158121542	21.552917190759647
25	17.09464121070422	26.776334716030846	34.00473204187868	22.12429203138625
26	16.479722032651164	27.096704622751933	35.65247663549658	20.77109670910032
27	17.509375838377746	25.367356373648477	36.81765146395913	20.305616324014647
28	16.62490713712959	24.14994326549695	37.51603995034378	21.709109647029674
29	16.540952759058026	22.248992267615215	38.68110283963557	22.52895213369119
30	18.568096515445554	22.83536695712394	37.86644686788603	20.730089659544472
31	17.674747306650268	25.02963589545926	35.69818513022444	21.597431667666033
32	17.14348400221341	24.786205512108648	35.54800007611863	22.522310409559307
33	17.479040323101223	23.404465701278383	35.59128322214664	23.52521075347375
34	16.595840265788368	22.87309045767076	34.444354478518314	26.08671479802256
35	14.851380228632024	22.747793212531068	35.78922898911093	26.611597569725976
36	16.193605512183275	23.542598637998797	36.03885333991038	24.22494250990755
37	17.128782657786658	24.332180235497628	35.655797497562524	22.88323960915319
38	16.54975864049131	24.58710304037982	34.90826771521331	23.954870603915555
39	16.812890317896052	23.203646828931202	34.132230757376696	25.85123209579605
40	17.403667948121416	23.10588661979898	32.88750449155923	26.602940940520377
41	15.994764231852887	24.274755440896676	32.68907365485497	27.041406672395464
42	17.161506208706108	24.839227365992954	34.43338443978362	23.565881985517308
43	18.17183185094777	23.798118451791716	36.13866576717324	21.891383930087272
44	18.673692466531122	23.63722455034968	32.75814012321518	24.93094285990402
45	19.15193391708367	22.220783596583168	32.02378184995898	26.603500636374182
46	19.04992001946249	22.729547127696847	32.95787691691166	25.262655935929
47	16.689719917000836	23.82722263618986	34.80931348825963	24.67374395854967
48	16.414274930812265	22.77581531827851	35.7047895412994	25.105120209609826
49	18.452202160649875	21.197771514988467	35.54348519623123	24.806541128130423
50	17.042179052673724	20.71647039376842	34.22249104206786	28.018859511489996
51	15.76357253117226	20.323451965222738	32.62676084979741	31.286214653807594
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	327.0
1	1001.0
2	1675.0
3	35807.0
4	69939.0
5	46834.5
6	23730.0
7	23880.5
8	24031.0
9	24856.0
10	25681.0
11	27067.5
12	28454.0
13	28651.0
14	28848.0
15	28079.0
16	27310.0
17	26650.5
18	25991.0
19	25342.5
20	24694.0
21	24161.0
22	23628.0
23	23477.0
24	23326.0
25	24641.0
26	28410.5
27	30865.0
28	34563.0
29	38261.0
30	42159.0
31	46057.0
32	51911.0
33	57765.0
34	64324.5
35	70884.0
36	77600.5
37	84317.0
38	94131.0
39	103945.0
40	116400.5
41	128856.0
42	145022.0
43	161188.0
44	185965.5
45	210743.0
46	262362.5
47	313982.0
48	331528.0
49	349074.0
50	313233.0
51	277392.0
52	221217.5
53	165043.0
54	131914.0
55	98785.0
56	80698.5
57	62612.0
58	52915.0
59	43218.0
60	37257.5
61	31297.0
62	26548.0
63	21799.0
64	17208.5
65	12618.0
66	9984.5
67	7351.0
68	5815.5
69	4280.0
70	3472.5
71	2665.0
72	2083.0
73	1501.0
74	1246.0
75	720.0
76	449.0
77	352.0
78	255.0
79	178.5
80	102.0
81	107.0
82	112.0
83	67.5
84	23.0
85	12.5
86	2.0
87	2.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2680027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.566081220461214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96139681140073	28.52401236607241
2	11.707920396719619	9.030572178851665
3	4.639073268262236	5.3673262935441555
4	2.307546281221038	3.559720692061751
5	1.3860989569031552	2.6728202475761824
6	0.9255846069798312	2.1417702677515704
7	0.6505622644665291	1.756274599126833
8	0.5137546469188342	1.5850802752368904
9	0.39783821363800115	1.3808754773789718
>10	3.2701440068721874	24.592782212844167
>50	0.15473696297621248	3.9785537974741847
>100	0.07486642497189346	5.361883465371928
>500	0.006266711727719193	1.6513712991131728
>1k	0.0029375211223681367	2.112871611174835
>5k	5.875042244736273E-4	1.604205446092441
>10k+	6.854215952192318E-4	4.679879770328947
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49056	1.8304293203016238	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	14714	0.5490243195311092	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	13407	0.5002561541357605	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	12828	0.47865189417867804	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	12747	0.47562953656810175	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	10974	0.40947348664770916	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCC	10202	0.38066780670493244	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	9629	0.3592874250893741	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	7814	0.2915642267783123	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC	7080	0.264176442998522	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	6084	0.22701263830550963	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	6054	0.22589324659788873	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGT	5820	0.2171619912784461	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG	4615	0.17219975768900836	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC	4571	0.17055798318449777	No Hit
TCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	4548	0.16969978287532178	No Hit
TTCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	4324	0.16134165812508605	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.985044553655616E-4	0.0	0.0	0.5302185388430788	0.0
2	2.985044553655616E-4	0.0	0.0	1.668565279379648	0.0
3	2.985044553655616E-4	0.0	0.0	2.604339433893763	0.0
4	2.985044553655616E-4	0.0	0.0	3.8290659012017416	0.0
5	2.985044553655616E-4	0.0	0.0	6.058110608587152	0.0
6	2.985044553655616E-4	0.0	0.0	8.358796385260298	0.0
7	2.985044553655616E-4	0.0	0.0	10.28094119947299	0.0
8	2.985044553655616E-4	0.0	0.0	12.545731815388427	0.0
9	2.985044553655616E-4	0.0	0.0	13.85874097537077	0.0
10	2.985044553655616E-4	0.0	0.0	15.718871488981268	0.0
11	3.358175122862568E-4	0.0	0.0	18.87279493825995	0.0
12	3.358175122862568E-4	0.0	0.0	21.299188403698917	0.0
13	3.73130569206952E-4	0.0	0.0	22.24451470078473	0.0
14	3.73130569206952E-4	0.0	0.0	22.595220122782344	0.0
15	3.73130569206952E-4	0.0	0.0	22.980477435488524	0.0
16	3.73130569206952E-4	0.0	0.0	23.77629031349311	0.0
17	3.73130569206952E-4	0.0	0.0	25.0358298629081	0.0
18	3.73130569206952E-4	0.0	0.0	26.40294295542545	0.0
19	3.73130569206952E-4	0.0	0.0	27.38099280343071	0.0
20	3.73130569206952E-4	0.0	0.0	28.351803918393358	0.0
21	3.73130569206952E-4	0.0	0.0	29.716864792780072	0.0
22	3.73130569206952E-4	0.0	0.0	31.197334952222498	0.0
23	3.73130569206952E-4	0.0	0.0	32.702133224777214	0.0
24	3.73130569206952E-4	0.0	0.0	33.73454819671593	0.0
25	3.73130569206952E-4	0.0	0.0	34.64438977667016	0.0
26	3.73130569206952E-4	0.0	0.0	35.43490420059201	0.0
27	3.73130569206952E-4	0.0	0.0	36.233926001491774	0.0
28	3.73130569206952E-4	0.0	0.0	37.076939896501045	0.0
29	3.73130569206952E-4	0.0	0.0	37.942677443175015	0.0
30	3.73130569206952E-4	0.0	0.0	38.860466704253355	0.0
31	3.73130569206952E-4	0.0	0.0	39.72079385767382	0.0
32	3.73130569206952E-4	0.0	0.0	40.52959167948681	0.0
33	4.4775668304834244E-4	0.0	0.0	41.329882124321884	0.0
34	4.850697399690376E-4	0.0	0.0	42.08894164125959	0.0
35	5.223827968897329E-4	0.0	0.0	42.895575305771175	0.0
36	5.59695853810428E-4	0.0	0.0	43.65463482270888	0.0
37	5.59695853810428E-4	0.0	0.0	44.393209471397114	0.0
38	5.59695853810428E-4	0.0	0.0	45.14118701042937	0.0
39	5.59695853810428E-4	0.0	0.0	45.921738848153396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGA	20	7.0349703E-4	45.0	12
CGCGGAA	35	1.2127384E-7	45.0	4
GTACGAG	25	3.8922022E-5	45.0	1
CGTTTTT	31205	0.0	44.509693	1
TACGGGT	60	3.6379788E-12	41.250004	4
CGTTTTC	1500	0.0	41.249996	1
CGTTTCT	1935	0.0	41.046513	1
TTCTCCG	420	0.0	40.714287	1
TACTTCG	45	1.9299478E-8	40.0	1
AGGGCGA	400	0.0	39.375	6
CGTTCTG	1465	0.0	39.163822	1
GTTTTTT	35145	0.0	39.03329	2
TTACGGG	615	0.0	38.780487	3
TACGGGA	390	0.0	38.653843	4
TTGGGAC	6920	0.0	38.49711	5
CTACGGG	235	0.0	38.29787	3
TTGCTTG	2220	0.0	38.10811	1
TTTCGCG	935	0.0	38.02139	1
GCGCGAC	160	0.0	37.96875	9
TCTACGG	160	0.0	37.96875	2
>>END_MODULE