##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2397513
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15284380105551	33.0	31.0	34.0	30.0	34.0
2	32.375900777180355	34.0	31.0	34.0	30.0	34.0
3	32.37742902749641	34.0	31.0	34.0	30.0	34.0
4	35.942324400326505	37.0	35.0	37.0	35.0	37.0
5	35.932500470278995	37.0	35.0	37.0	35.0	37.0
6	35.639379223386904	37.0	35.0	37.0	35.0	37.0
7	35.979560903319395	37.0	35.0	37.0	35.0	37.0
8	36.064543967019155	37.0	35.0	37.0	35.0	37.0
9	37.86762157285487	39.0	38.0	39.0	35.0	39.0
10	37.297874505789956	39.0	37.0	39.0	34.0	39.0
11	37.16075866950461	39.0	37.0	39.0	34.0	39.0
12	37.09089418910346	39.0	37.0	39.0	33.0	39.0
13	37.091618689867374	39.0	37.0	39.0	33.0	39.0
14	38.309396862498765	40.0	38.0	41.0	33.0	41.0
15	38.32499510951557	40.0	38.0	41.0	33.0	41.0
16	38.32646830277876	40.0	38.0	41.0	34.0	41.0
17	38.258789003438146	40.0	38.0	41.0	33.0	41.0
18	38.132807621898195	40.0	37.0	41.0	33.0	41.0
19	38.05944409894754	40.0	37.0	41.0	34.0	41.0
20	37.915988359604306	40.0	36.0	41.0	34.0	41.0
21	37.76279753227615	39.0	36.0	41.0	33.0	41.0
22	37.852565137290185	39.0	36.0	41.0	33.0	41.0
23	37.78438782188042	39.0	36.0	41.0	33.0	41.0
24	37.73121522177356	39.0	36.0	41.0	33.0	41.0
25	37.6489466376199	39.0	35.0	41.0	33.0	41.0
26	37.58694864219714	39.0	35.0	41.0	33.0	41.0
27	37.5121803302005	39.0	35.0	41.0	33.0	41.0
28	37.39220642390677	39.0	35.0	41.0	33.0	41.0
29	37.29697357219752	39.0	35.0	41.0	33.0	41.0
30	37.20870460347869	39.0	35.0	41.0	32.0	41.0
31	37.04327400935887	39.0	35.0	41.0	32.0	41.0
32	36.75271666931525	39.0	35.0	41.0	31.0	41.0
33	36.480318980543586	39.0	35.0	41.0	30.0	41.0
34	36.183789618659	39.0	35.0	41.0	29.0	41.0
35	36.00904395513184	39.0	35.0	41.0	28.0	41.0
36	35.80171619507381	39.0	35.0	41.0	27.0	41.0
37	35.77285545479837	39.0	35.0	41.0	27.0	41.0
38	35.688733700296936	39.0	35.0	41.0	26.0	41.0
39	35.5787096879141	39.0	35.0	40.0	25.0	41.0
40	35.46307736391836	39.0	35.0	40.0	25.0	41.0
41	35.39612423373721	39.0	35.0	40.0	25.0	41.0
42	35.33783466450443	39.0	35.0	40.0	24.0	41.0
43	35.28306958085316	38.0	35.0	40.0	24.0	41.0
44	35.13649727863832	38.0	35.0	40.0	24.0	41.0
45	35.173618245239965	38.0	35.0	40.0	24.0	41.0
46	35.05987788178834	38.0	34.0	40.0	23.0	41.0
47	34.87177879744552	38.0	34.0	40.0	23.0	41.0
48	34.7707707945692	38.0	34.0	40.0	23.0	41.0
49	34.73324941303759	38.0	34.0	40.0	23.0	41.0
50	34.634422420232966	38.0	34.0	40.0	23.0	41.0
51	33.71652958711798	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	25.0
11	22.0
12	18.0
13	24.0
14	25.0
15	59.0
16	103.0
17	256.0
18	587.0
19	1135.0
20	2219.0
21	3527.0
22	5995.0
23	9619.0
24	16318.0
25	28066.0
26	38940.0
27	40913.0
28	39006.0
29	38772.0
30	43148.0
31	50937.0
32	64579.0
33	86047.0
34	132968.0
35	167611.0
36	199879.0
37	299216.0
38	513523.0
39	613748.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.57060253687884	4.157266300537265	56.69345692807505	17.578674234508842
2	24.574381869879332	3.6657986838861767	51.351045854600166	20.40877359163433
3	18.399316291507073	3.8855263767078636	56.92273618537209	20.79242114641297
4	16.11736828955672	4.0931582018533375	56.59185163959486	23.197621868995082
5	16.314572642567526	5.1437468743652275	56.40728538281127	22.134395100255976
6	20.015032243829335	5.428875672415541	57.595433267723685	16.960658816031447
7	80.57945879751226	1.398240593481662	14.13514754664521	3.8871530623608717
8	83.23554449965444	1.7434316310276525	11.814159089022667	3.2068647802952475
9	78.62597616780388	4.209028272213748	13.28293110402321	3.882064455959154
10	35.127442478935464	32.5483949409242	20.185917657172244	12.138244922968093
11	22.23266359765307	25.838692011263337	33.417045079630434	18.51159931145316
12	20.779449371077447	22.476541315938643	36.71483741693997	20.029171896043945
13	21.172731910108517	22.570763954147484	37.577189362476865	18.67931477326713
14	19.23914489723309	25.118195396646442	35.69945189035471	19.943207815765753
15	17.278321327141917	25.80615829820318	37.08834946880371	19.827170905851187
16	19.721770017513983	25.9830916453842	35.07372014249766	19.22141819460416
17	20.083269621478593	25.554522540649415	33.83389370568585	20.528314132186146
18	20.864704383250476	24.745392412887853	34.66896738411846	19.72093581974321
19	21.094984677872443	26.86458842976034	33.214585280663755	18.82584161170346
20	22.836330814473165	26.81770651504288	32.7627420581244	17.583220612359558
21	22.209556319402648	26.490325599902896	33.502550351134694	17.797567729559756
22	21.88050700872112	23.574512421830455	33.740755524579015	20.804225044869412
23	20.205813274005187	26.165322148409626	32.68069870736884	20.948165870216346
24	19.938119209364036	25.55114403967778	33.960274667958004	20.550462083000177
25	19.991591286470605	27.27442979454126	31.523040750978197	21.210938168009932
26	19.588632053298564	28.096531697638344	31.466648981673927	20.848187267389164
27	19.1601880782294	27.45565926024176	32.863346309279656	20.520806352249185
28	17.671311896953217	26.98483803841731	33.61633492706817	21.727515137561298
29	18.445655977673532	26.095583214772976	33.89591630994285	21.56284449761065
30	19.976283757376915	25.7873054285837	32.983846177267864	21.25256463677152
31	20.00543896946544	28.4401794693084	30.254392781186173	21.299988780039982
32	19.93273863374255	28.940364452664074	29.922632327749632	21.204264585843745
33	20.355510063970456	27.566774403308763	29.877335388796638	22.200380143924143
34	19.14704946333972	27.855365122107784	30.266905747747767	22.730679666804726
35	19.309634608863433	27.901663098385704	29.524344602093922	23.264357690656944
36	19.252825740673774	29.770850043357427	28.613609185852173	22.362715030116625
37	19.305255070566876	29.764301590856856	28.765891988906837	22.164551349669427
38	20.40860675208018	29.53085134470595	29.021198216652007	21.039343686561867
39	21.47316823725252	26.132287916687	30.287760692017102	22.106783154043377
40	20.86745723589403	26.595142549800567	30.032329334606317	22.505070879699087
41	18.92778057929196	28.200055640991312	28.905077886960363	23.96708589275637
42	20.869751279763655	28.810646699308823	27.588505255237404	22.73109676569011
43	21.093107732888207	27.354596200312574	29.023242001190404	22.52905406560882
44	20.474258116640033	26.93929084013309	28.455486998402097	24.130964044824783
45	20.926893827061626	26.02392562626355	27.919806899900024	25.129373646774805
46	20.37565594013463	26.93516156116776	28.416738511949674	24.272443986747934
47	18.777583270664223	28.464621463992064	29.656940337758336	23.100854927585377
48	19.713970268357254	27.19059291857854	29.606930181400475	23.48850663166373
49	20.921262992108904	25.8062834278688	30.33314105074717	22.939312529275128
50	20.106502029394626	25.440821384493013	29.627117767453193	24.825558818659168
51	18.790930434996596	25.320446646170424	28.60314000382897	27.285482915004007
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	403.0
1	1139.5
2	1876.0
3	22025.5
4	42175.0
5	28810.5
6	15446.0
7	15556.5
8	15667.0
9	16326.0
10	16985.0
11	17277.0
12	17569.0
13	17563.0
14	17557.0
15	16828.0
16	16099.0
17	15528.5
18	14958.0
19	14637.0
20	14316.0
21	14347.5
22	14379.0
23	15167.0
24	15955.0
25	17688.5
26	22520.0
27	25618.0
28	30318.0
29	35018.0
30	40553.0
31	46088.0
32	53002.0
33	59916.0
34	68061.0
35	76206.0
36	82397.0
37	88588.0
38	97264.0
39	105940.0
40	118135.5
41	130331.0
42	140681.5
43	151032.0
44	165972.0
45	180912.0
46	201781.5
47	222651.0
48	236516.5
49	250382.0
50	235505.0
51	220628.0
52	190915.0
53	161202.0
54	137854.5
55	114507.0
56	100142.0
57	85777.0
58	77202.5
59	68628.0
60	61737.5
61	54847.0
62	45920.5
63	36994.0
64	30482.0
65	23970.0
66	18932.0
67	13894.0
68	10719.5
69	7545.0
70	6306.0
71	5067.0
72	4255.5
73	3444.0
74	2948.0
75	2086.5
76	1721.0
77	1218.0
78	715.0
79	532.5
80	350.0
81	241.5
82	133.0
83	119.0
84	105.0
85	64.5
86	24.0
87	19.5
88	15.0
89	9.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2397513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.04451415278561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27431421655183	24.21314113164507
2	11.470800840162939	7.580940814130986
3	4.451787725040777	4.413214874559217
4	2.225531472517926	2.9416642496435363
5	1.326700691133186	2.192008988233051
6	0.8592426226800824	1.7035953003497166
7	0.6245734798328902	1.444710903565475
8	0.48436111410740124	1.280438215214482
9	0.38945528915714256	1.1582424732987369
>10	4.240353819440102	32.29474335666993
>50	0.5631292671937025	11.868730111565503
>100	0.08414111943019931	4.962579958972142
>500	0.002804171375967954	0.6194486836132627
>1k	0.0024217843701541423	1.642863663856348
>5k	2.549246705425413E-4	0.5741283066792944
>10k+	1.2746233527127066E-4	1.1095489680032757
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26343	1.0987635937740483	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	7330	0.3057334829884134	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	6301	0.26281400768212726	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	4420	0.18435770734089868	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	4388	0.1830229909076614	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	4021	0.1677154618139714	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	2898	0.12087525698505075	No Hit
TCCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	2466	0.10285658513634753	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6683955415465942E-4	0.0	0.0	0.32917444034714305	0.0
2	1.6683955415465942E-4	0.0	0.0	1.01530210680818	0.0
3	1.6683955415465942E-4	0.0	0.0	1.5450176912492237	0.0
4	1.6683955415465942E-4	0.0	0.0	2.1500196245025576	0.0
5	1.6683955415465942E-4	0.0	0.0	3.170577177266609	0.0
6	1.6683955415465942E-4	0.0	0.0	4.052699609970833	0.0
7	1.6683955415465942E-4	0.0	0.0	4.786209709811793	0.0
8	1.6683955415465942E-4	0.0	0.0	5.780865421793333	0.0
9	2.0854944269332428E-4	0.0	0.0	6.29894394733209	0.0
10	2.0854944269332428E-4	0.0	0.0	7.164382424620847	0.0
11	2.0854944269332428E-4	0.0	0.0	8.96462292383816	0.0
12	2.0854944269332428E-4	0.0	0.0	10.456919315974512	0.0
13	2.0854944269332428E-4	0.0	0.0	11.009283369892051	0.0
14	2.502593312319892E-4	0.0	0.0	11.206529432791397	0.0
15	2.502593312319892E-4	0.0	0.0	11.447404039102187	0.0
16	2.502593312319892E-4	0.0	0.0	12.02742174912086	0.0
17	2.91969219770654E-4	0.0	0.0	12.936029961047135	0.0
18	2.91969219770654E-4	0.0	0.0	13.90874627165734	0.0
19	2.91969219770654E-4	0.0	0.0	14.648179175670789	0.0
20	2.91969219770654E-4	0.0	0.0	15.382481763393983	0.0
21	2.91969219770654E-4	0.0	0.0	16.44283054982392	0.0
22	2.91969219770654E-4	0.0	0.0	17.620175573604815	0.0
23	2.91969219770654E-4	0.0	0.0	18.801024228022957	0.0
24	2.91969219770654E-4	0.0	0.0	19.63618132623264	0.0
25	2.91969219770654E-4	0.0	0.0	20.378700761997955	0.0
26	2.91969219770654E-4	0.0	0.0	21.030000671529205	0.0
27	2.91969219770654E-4	0.0	0.0	21.690935565312888	0.0
28	2.91969219770654E-4	0.0	0.0	22.399878540804576	0.0
29	2.91969219770654E-4	0.0	0.0	23.14352414356043	0.0
30	2.91969219770654E-4	0.0	0.0	23.945938979267265	0.0
31	2.91969219770654E-4	0.0	0.0	24.697050652071542	0.0
32	2.91969219770654E-4	0.0	0.0	25.413251148168957	0.0
33	2.91969219770654E-4	0.0	0.0	26.101756278276696	0.0
34	2.91969219770654E-4	0.0	0.0	26.79922903442025	0.0
35	2.91969219770654E-4	0.0	0.0	27.55497050485232	0.0
36	2.91969219770654E-4	0.0	0.0	28.280972824756322	0.0
37	2.91969219770654E-4	0.0	0.0	28.992835492445714	0.0
38	4.1709888538664857E-4	0.0	0.0	29.698858775739694	0.0
39	4.5880877392531343E-4	0.0	0.0	30.45188910341675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.0348225E-4	45.0	10
TATTCGC	25	3.8920778E-5	45.0	25
CGTAAGG	30	2.1664928E-6	44.999996	2
CGTTTTT	18420	0.0	44.254887	1
GTTAGCG	95	0.0	42.63158	1
TAGCGCG	75	0.0	42.0	1
ACTTGCG	65	0.0	41.53846	1
AGTTTCG	35	6.25113E-6	38.571426	1
AATTCGG	35	6.25113E-6	38.571426	23
CCGTTCG	35	6.25113E-6	38.571426	1
TGGGCGA	3155	0.0	38.153725	6
TTTCGCG	655	0.0	38.129772	1
TTGGGAC	6580	0.0	37.5114	5
TCGTTAG	90	0.0	37.500004	1
TACGGGA	390	0.0	37.5	4
TCGCGCG	120	0.0	37.499996	1
TTTGGGA	17690	0.0	37.457603	4
GTTTTTT	22190	0.0	37.364803	2
TTGGGCG	2560	0.0	37.353516	5
GGCACCG	1010	0.0	37.202972	8
>>END_MODULE