Basic Statistics
Measure | Value |
---|---|
Filename | SRR3549725_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 792969 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC | 2820 | 0.35562550364516143 | TruSeq Adapter, Index 13 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT | 2620 | 0.33040383671997264 | TruSeq Adapter, Index 19 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC | 2126 | 0.26810631941475643 | TruSeq Adapter, Index 13 (95% over 21bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2005 | 0.2528472109250173 | No Hit |
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC | 1862 | 0.2348137190735073 | TruSeq Adapter, Index 13 (95% over 21bp) |
TCCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG | 980 | 0.12358616793342488 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCTATCG | 30 | 2.1646465E-6 | 45.000004 | 1 |
CGGTAGT | 30 | 2.1646465E-6 | 45.000004 | 12 |
TCGCAAC | 20 | 7.031963E-4 | 45.0 | 16 |
CATATCG | 20 | 7.031963E-4 | 45.0 | 27 |
GATCGAC | 20 | 7.031963E-4 | 45.0 | 15 |
CAACGAA | 20 | 7.031963E-4 | 45.0 | 17 |
GACGTCC | 20 | 7.031963E-4 | 45.0 | 28 |
CGTTGAT | 25 | 3.8897077E-5 | 45.0 | 25 |
TCGGATT | 20 | 7.031963E-4 | 45.0 | 31 |
CGAATTG | 20 | 7.031963E-4 | 45.0 | 27 |
CGAATAC | 20 | 7.031963E-4 | 45.0 | 28 |
AGACACG | 35 | 1.2112832E-7 | 45.0 | 10 |
CGTCGGT | 25 | 3.8897077E-5 | 45.0 | 43 |
GACGCAT | 25 | 3.8897077E-5 | 45.0 | 38 |
TGTCGCG | 35 | 1.2112832E-7 | 45.0 | 1 |
ATCGCTA | 25 | 3.8897077E-5 | 45.0 | 14 |
TCGGTAC | 25 | 3.8897077E-5 | 45.0 | 45 |
CCGCACG | 20 | 7.031963E-4 | 45.0 | 22 |
TCGCGCG | 20 | 7.031963E-4 | 45.0 | 1 |
TCGCGAG | 25 | 3.8897077E-5 | 45.0 | 1 |