FastQCFastQC Report
Sat 18 Jun 2016
SRR3549725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792969
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC28200.35562550364516143TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT26200.33040383671997264TruSeq Adapter, Index 19 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC21260.26810631941475643TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.2528472109250173No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC18620.2348137190735073TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTG9800.12358616793342488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG302.1646465E-645.0000041
CGGTAGT302.1646465E-645.00000412
TCGCAAC207.031963E-445.016
CATATCG207.031963E-445.027
GATCGAC207.031963E-445.015
CAACGAA207.031963E-445.017
GACGTCC207.031963E-445.028
CGTTGAT253.8897077E-545.025
TCGGATT207.031963E-445.031
CGAATTG207.031963E-445.027
CGAATAC207.031963E-445.028
AGACACG351.2112832E-745.010
CGTCGGT253.8897077E-545.043
GACGCAT253.8897077E-545.038
TGTCGCG351.2112832E-745.01
ATCGCTA253.8897077E-545.014
TCGGTAC253.8897077E-545.045
CCGCACG207.031963E-445.022
TCGCGCG207.031963E-445.01
TCGCGAG253.8897077E-545.01